The application discloses a 3D bioprinting
bacteria-
algae symbiotic
engineering living material and a preparation method and application thereof. The method takes
chlorella as a biological
oxygen supply source, takes alkali-producing
bacteria as
phenol-degrading
bacteria, takes
sodium alginate and
carboxymethyl cellulose as carriers, and respectively prepares bacterial bio-ink and algal bio-ink added with
silicon dioxide, so as to build bacteria-
algae symbiotic
engineering living materials with stripe and layer configurations through a 3D bioprinting mechanism. The bacteria-
algae symbiotic
engineering living material realizes three-dimensional accurate arrangement and functional partition of bacteria and algae, and exhibits excellent degradation performance and photosynthetic performance under different
phenol concentrations, and has application prospects in the biological degradation treatment of organic pollutants.