109 results about "Algae organisms" patented technology

A method of fractionating biomass, by permeability conditioning biomass suspended in a pH adjusted solution of at least one water-based polar solvent to form a conditioned biomass, intimately contacting the pH adjusted solution with at least one non-polar solvent, partitioning to obtain an non-polar solvent solution and a polar biomass solution, and recovering cell and cell derived products from the non-polar solvent solution and polar biomass solution. Products recovered from the above method. A method of operating a renewable and sustainable plant for growing and processing algae.

A novel low cost separation process for separating lipid oil from an algal biomass for biofuel production is described herein. The process of the present invention comprises of two steps: (i) breaking the algae cells and (ii) separation of the lipid oils from the broken cells. The separated lipids are extracted by conventional techniques followed by conversion to a biofuel.

Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.

Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.

Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.

A method of isolating nutraceuticals products from algae is provided. A method of isolating carotenoids and omega-3 rich oil from algae includes dewatering substantially intact algal cells to make an algal biomass and adding a first ethanol fraction to the algal biomass. The method also includes separating a first substantially solid biomass fraction from a first substantially liquid fraction comprising proteins and combining the first substantially solid biomass fraction with a second ethanol fraction. The method further includes separating a second substantially solid biomass fraction from a second substantially liquid fraction comprising polar lipids and combining the second substantially solid biomass fraction with a third ethanol solvent fraction. The method also includes separating a third substantially solid biomass fraction from a third substantially liquid fraction comprising neutral lipids, wherein the third substantially solid biomass fraction comprises carbohydrates and separating the neutral lipids into carotenoids and omega-3 rich oil.

A controlled eutrophication system and process are disclosed. The system includes the combination of a partitioned aquaculture system in conjunction with an anaerobic digester. Wastewater containing pollutants, such as nitrogen and phosphorus, are fed to the partitioned aquaculture system. Algae within the system converts the pollutants into algal biomass. Fish populations, in turn, control the algal populations. The fish populations may then be periodically harvested for human or animal consumption. A polishing chamber is contained in the system in which aquatic organisms remove substantial amounts of the algae from batch fed additions of water. The water is then discharged to an external water source containing virtually no pollutants. In one embodiment, the biomass excreted by the aquatic organisms in the system are collected and fed to a digester. In the digester, the biomass is converted to a hydrocarbon gas and collected for its fuel value, while the liquid fraction is collected for its fertilizer value.

The invention provides a device for separating a first entity and a second entity by flowing them downwardly in an inclined settling chamber. Each entity has its own outlet located at approximately the lowest end of the inclined settling chamber. The device may be used in industrial fields such as pharmaceutics, biologics, and biofuels, for the purposes of large-scale growth and separation of algae biomass, bacteria and yeast cultures; algae metabolite production; and cell separation, among others. The invention exhibits technical merits such as effective particle separation or concentration capacity, robust structure, easy operation, cost-effective manufacturability, disposability, and high productivity in e.g. perfusion photobioreactor systems.

The present invention provides a tissue culture method for cultivation of marine algae, said method comprising the steps of (i) establishing axenic viable algal material by sequential treatment thereof in sterile sea water supplemented with domestic liquid detergent, incubating the treated material, (ii) culturing the axenic explants on agarified medium for induction of callus; (iii) excising and subculturing the calli from the axenic explants on fresh agar plates to obtain differentiated densely pigmented oval or spherical shaped micro-propagules (iv) subculturing the pigmented calli in agarified medium to achieve enhanced somatic embryogenesis and micro-propagule formation in pigmented filamentous callus, (v) transferring the filamentous calli with somatic embryos for morphogenesis and development of young plantlets; and (vi) cultivating algal biomass on a large scale by growing the young plantlets in enclosed perforated polythene bags.

The invention relates to systems and methods for regulating algal biomass in offshore waters near an oil and gas production platform. The systems of the invention encompasses a plurality of modules for managing nutrients, algae, and aquaculture, including enclosures for containing aquatic organisms, and various operating subsystems that are operably associated with surface and underwater structures of the platform. In one embodiment of the invention, aquatic organisms are cultured in eutrophic water to feed on algae, thereby reducing the algal biomass. In other embodiments, the diversity of algae in an algal bloom is modified and the productivity of oligotrophic water is increased.

The invention relates to an algae organism culture technology, in particular to a method for quickly growing chlorella and accumulating a large amount of biological grease through mixotrophic culture. In the method, two-stage culture is adopted, namely at the first stage, high biomass is obtained through the autotrophic aerated culture of the chlorella, and at the second stage, an aerated heterotrophic culture mode of adding organic matters is adopted, the single nutrition mode of the chlorella is changed, and the grease is quickly accumulated on the basis of a large amount of biomass; meanwhile, a novel air sterilization method is adopted to effectively solve the problem of bacterial contamination of culture solution, and culture cost is reduced because a filter membrane is not needed; and algae powder grease is extracted by an alkali-catalyzed anhydrous diethyl ether-petroleum ether extraction method. The method has the advantages that: the whole process is easy to operate, experiment cost and production cost are low, the culture period is short, the grease accumulation concentration of microalgae can be quickly improved, and the extracted grease has high content and purity and is not required to be decolorized; and the method is suitable for preparing microalgae grease in a laboratory or in the industry, and has a good application prospect.

The invention belongs to the technical field of environmental protection, and particularly relates to a preparation method of an algae biofilm and application thereof in sewage treatment. The preparation method specifically comprises the steps of carrier pretreating, algae culturing, algae domesticating and algae biofilm preparing. The preparation method of the algae biofilm has the advantages that the preparation cost is low, the raw materials are easy to obtain, and the algae biomass is easy to recycle; the prepared algae biofilm can achieve the efficient nitrogen and phosphorus removal effect in sewage treatment, achieves the purpose of general sewage treatment and not only can overcome the defects that in a suspended algae system, the algae density is low, the hydraulic retention time is long, algae and water are difficult to separate, too more suspended solids (SS) exist, the resource recycling rate is low but also has the advantages of reducing immobilization reagent cost, weakening the influences of immobilization reagents on the algae activity, reusing immobilization carriers and the like.

The invention relates to a method for preparing algae organism organic feed, in particular to the technical field of the comprehensive utilization and the comprehensive treatment of waste residues which are produced after the production of an algae chemical enterprise. The method comprises the following steps: processing auxiliary materials, selecting a fermenting place, fermenting, sieving and pelletizing. The creative method has the advantages that the steps are simple, the operation is easy, the cost is low, and large-scale production and management are facilitated. A product produced by the creative method is rich in algae extract which is easily absorbed by plants and quickly takes effect, so that the quality and the yield of crops are improved. The product is rich in organic substances which are used for promoting the forming of soil granular structure and improving soil quality. The product comprises a large quantity of biological effective bacterial floras which can fertilize soil, take roots, resist diseases, kill bacteria, and improve the utilization rate of the feed.

A closed-loop system for growing algae in a bioreactor is disclosed, for example, to produce biofuel. An aqueous bioreactor solution is formulated such that the principal source of carbon for algae growth is supplied by sodium bicarbonate. During algae growth in the aqueous solution, the concentration of sodium bicarbonate in the solution is reduced while the concentration of sodium carbonate increases. A regenerator is provided to regenerate sodium bicarbonate from the sodium carbonate. Specifically, after sufficient growth, the algae are concentrated and an algae depleted media is produced. Carbon dioxide is then introduced into the algae depleted media to regenerate sodium bicarbonate from the sodium carbonate. The regenerated sodium bicarbonate is then recycled into the bioreactor to supply carbon for further algae growth.

Disclosed are cross-flow membrane filtration methods for the removal or separation of algal cells from an aqueous environment. The methods of the invention may be used for the simultaneous algal harvesting / dewatering and water / wastewater purification and recycling.

A self-contained system which promotes the multiplication of algae from either salt water or fresh water sources as a feedstock. This process inspires a direct output of lipids for bio-diesel fuel as well as a significant raw algae yield which can be further processed into additional bio-fuel as well as other valuable resources. In addition to inspiring a direct output of algal lipids for bio-diesel, the system generates massive raw algae biomass output that can be directly utilized as an agent for efficiently cleaning up oil spills.

The invention belongs to the technical field of environmental engineering, and in particular, relates to an application of filamentous oil producing alga tribonema in removal and degradation of industrial phenol pollutants in an environment, and specifically, relates to the application of the filamentous oil producing alga in treatment of industrial phenol pollution in the environment. Compared with unicellular algae, the used filamentous alga has the advantages of easy recovery, strong stress resistance and the like, can directly achieve efficient recovery through filter cloth filtration, low-speed centrifugation and other methods without addition of a flocculant, and prevents water body secondary pollution; at the same time, the tribonema has excellent abilities to absorb and degrade phenol and derivatives thereof and can continuously reduce the phenol concentration to 1-2 mg / L from phenol wastewater with the concentration of 200-800 mg / L, the removal rate of the phenol pollutants can reach 99%, and the oil content of obtained algal biomass can reach 40-50%, and the need of extraction of biological oil can be fully met. The method provides an efficient and low-cost way for resource utilization of industrial phenol-containing wastewater.

The invention discloses a bacteria and algae composite biological membrane sewage treatment device, and relates to the technical field of water treatment. The device comprises a treatment box, an airpump and a water pump, the air pump is communicated with an external air source, the water pump is communicated with an external sewage source, a sealing cover plate is hermetically connected at the top of the treatment box, an aeration ring pipe is fixedly mounted in a net box, a connecting air nozzle extends out of the net box and is communicated at the bottom of the aeration ring pipe, and a plurality of fluorescent lamps are electrically connected with a solar panel and uniformly arranged on one side, close to the treatment box, of a lamp frame. An aeration component in the device and thetreatment box can be rapidly disassembled and assembled, cleaning and maintaining convenience is greatly improved, light emitted by a fluorescent lamp provides illumination for the respiratory actionof bacterium and algae organisms, rapid growth and metabolism of the bacterium and algae organisms is facilitated, sewage purification is accelerated, air flow generated by aeration is dispersed and buffered by the net box, high air flow impact is avoided, sewage is sufficiently purified by bacteria and algae on a biological membrane, and sewage treatment efficiency is effectively improved.

A method of producing mineral-rich algae by growing algae with an algae biofilm growing apparatus, wherein the algae is fed a mineral-rich feed stock. Furthermore, the mineral-rich algae is harvested and used as a foodstuff for human and animal consumption.

The invention relates to a mountainous area unpowered algae biological rotating disc sewage treatment system. The system comprises a grating adjusting tank, an algae biological rotating disc and an inclined plate sedimentation tank; the system is constructed in a stepped mode from high to low according to mountainous terrain, the algae biological rotating disc comprises a waterwheel and biologicalrotating discs, and the waterwheel is arranged in the middle of the algae biological rotating disc, the biological rotating discs are distributed on the two sides of the waterwheel, and the waterwheel is connected with the biological rotating discs through bearings; a water outlet pipe is arranged above the waterwheel, the waterwheel is impacted by water flow, and the waterwheel drives the biological rotating discs to rotate through the bearings; green algae are inoculated in the biological rotating discs, and a bacteria-algae symbiotic system is formed on the biological rotating discs. The sewage treatment system has the beneficial effects of simple structure, investment saving, good treatment effect, small occupied area, no need of electric power, no power, no operation cost, no need ofspecially-assigned person management and the like, and is quite suitable for meeting the use requirements of domestic sewage treatment in rural areas and villages and towns in remote mountainous areas which cannot ensure electric power supply and have relatively backward economic conditions.

The invention discloses preparation of an algae biological membrane and an application of the algae biological membrane in urban secondary effluent treatment. The preparation method comprises the following steps: (1) performing expanded culture of microalgae; (2) performing biological membrane culturing of the microalgae; and (3) domesticating the algae membrane. Compared with a suspended algae technology, a technology for treating the urban secondary effluent by utilizing the algae biological membrane prepared from autotrophic microalgae has the advantages of simplicity in operation, stability in operation, easiness in culture and harvesting, reusability of a fixed carrier and the like, does not need any immobilized medicament, hardly generates negative effects on the environment, and hasgood application prospects in the sewage treatment.

A multitrophic, inland aquaculture system allows for the cultivation of aquatic animals as a food source and the cultivation of algae for use as biofuel and biomass in a sustainable manner based on the symbiotic nature of the organisms grown. This unique process minimizes biological, energy, and water inputs to create a highly sustainable system by utilizing the cyclic conversion of oxygen to carbon dioxide and back to oxygen as well as using the biological waste from aquatic food organisms to provide nutrients for algae organisms for the benefit of both species. This enables the cultivation of food species in higher densities than previously possible, and eliminates the need to provide additional nutrients, water, and non-atmospheric carbon dioxide for algal growth.

The invention belongs to the technical field of biological energy and genetic engineering, in particular relates to algae grease and a method for producing biodiesel thereby. The novel method for expressing FAEES by recombinant algae and producing in-vivo bio diesel comprises a method for preparing the biodiesel by using grease in algae body and (external source) ethanol to perform ester exchange reaction, namely the algae organisms are soaked in a culture medium containing ethanol, FAEES gene is induced into a gene group in the algae body by using agrobacterium rhizogenes as medium for expressing, and the FAEES produced in the algae body catalyzes fatty acid of the algae body per se to react with externally added (external source) ethanol under certain conditions to obtain the biodiesel and the side product glycerol. The method has mild technical condition, low cost and easy control, and has excellent biological industrial application prospect by using in-vivo grease in the algae organisms and in-vitro ethanol.

The invention discloses a floating island for ecological restoration of lake water in a smart city. The floating island comprises a floating island body, wherein a plurality of first planting groovesare formed in the upper wall of the floating island body; a first cavity is formed in the floating island body; a through groove is formed in the upper wall of the floating island body; the through groove communicates with the inner top of the first cavity; the inner wall of the through groove is rotationally connected with a first rotating rod; one end of the first rotating rod extends into the first cavity; two wind shields are fixedly connected to the side wall of the other end of the first rotating rod; a rotatable propeller is arranged on the side wall of the floating island body; a driving mechanism for driving the propeller to rotate is arranged in the floating island body; and the driving mechanism comprises two connecting rods arranged in the first cavity. According to the floating island, a shielding area can be increased, the growth of algae in water can be effectively inhibited, and the purification area of the floating island body can be increased; and meanwhile, a power source for driving the propeller does not need to be arranged, the purpose of saving resources is achieved, and the cost of the device is reduced.

The invention discloses a culture method for improving microalgae carbon fixing and oil producing capacity, and relates to the technical field of algae organism culture. According to different demands of microalgae on inorganic carbon sources in different stages (lag phase, the incipient stage of logarithmic phase and the middle and later periods of the logarithmic phase) of a growth cycle, the culture method gradually increases the supply of carbon dioxide, not only greatly improves the growth rate of the microalgae and the tolerance of carbon dioxide concentration, but also greatly improves the microalgae carbon fixing and oil producing capacity. The carbon fixing amount in of the microalgae cultured in a progressive-increase manner is respectively increased by 15 times and 32% compared with that of the microalgae cultured in continuous high-concentration carbon dioxide of 18-22% and continuous air. The oil contents of the microalgae cultured in the continuous high-concentration carbon dioxide of 18-22%, in the continuous air and in the progressive-increase manner are respectively 29%, 24% and 47%. The oil producing amount of the microalgae cultured in the progressive-increase manner is respectively increased by 7.7 times and 1.6 times compared with that of the microalgae cultured in the continuous high-concentration carbon dioxide of 18-22% and the continuous air.

The invention belongs to the field of algae biotechnology, in particular relates to a method of conservation and recovery of haematococcus pluvialis cells. After the cold acclimation of a concentrate solution of haematococcus pluvialis cells under 0-10 centigrade, the solution is subject to frosting and preserving in shading conditions and below the freezing point. By the method of conservation and recovery of haematococcus pluvialis cells, the seaweed concentrate solution after the short-time cold acclimation is subject to frosting and preserving, the middle-long term preservation method of high survival rate of the haematococcus pluvialis cells is achieved; at the same time the method of recovery of the preserved cells is achieved; the survival rate of cell recovery is high, after the preservation of 75 days, the survival rate of recovered cells is up to 95% or above, the growth after the recovery is fast; the propagation of bacteria and miscellaneous algae is effectively prevented, at the long-term preservation stage no obvious bacteria and miscellaneous algae are bred, the cells after the recovery are grown rapidly, and the proliferation of bacteria and miscellaneous algae is inhibited. The method is simple, does not require the assistance of specialized equipment; the method is tended to the preservation of a large number of algae species, and is easy to carry out in production and in practice.

The invention discloses integrated sewage treatment equipment. The equipment comprises a water storage tank, a coagulating sedimentation device, a pre-demulsification device, an algae biological membrane circulation assembly, a tubular membrane filtration assembly and a purified water tank, wherein a plate frame fiber membrane assembly is arranged in the pre-demulsification device and is used forintroducing ozone, the ozone and oil stains in the sewage are enriched on the surface of a fiber membrane under ultrasonic vibration, and the contact rate of the ozone and the oil stains in the sewageis increased, so that the demulsification efficiency is improved, and the operation burden of the subsequent algae biological membrane circulation assembly is reduced. The algae biological membrane circulation assembly is formed by connecting a plurality of algae biological membrane columns end to end in series, the algae biological membrane columns are filled with foaming polylactic acid carriers loaded with alkaline pectinase, algae propagation can be promoted, the disease resistance and adaptability of the algae can be improved, and the sewage treatment capacity of the algae biological membrane circulation assembly is improved. In a word, the integrated sewage treatment equipment disclosed by the invention is excellent in oil removal, nitrogen removal and phosphorus removal effects.

The invention relates to the technical field of capacitor electrode production, in particular to a preparation method of nitrogen-doped porous biochar and a method for preparing an electrode material, and the method comprises the following steps: (1) raw material pretreatment: mixing and pretreating algae biomass and terrestrial biomass; (2) adding an activating agent: mixing the pretreated raw materials with the activating agent; and (3) carbonizing and pickling: carbonizing the mixture obtained in the step (2) under the protection of inert gas, pickling the obtained biochar until the biochar is neutral after the reaction is finished, and drying to obtain the nitrogen-doped porous biochar.

The invention relates to the technical field of algal organism cultivation, in particular to an algal organism cultivation system and method.The algal organism cultivation system comprises a cultivation box, a base, a scraping mechanism and a feeding mechanism, in the scraping mechanism, an annular plate moves upwards, chlorella attached to the outer side wall of the cultivation box can be scraped through an annular scraping plate, and the feeding mechanism is used for feeding the chlorella; after the chlorella is scraped, an annular plate moves downwards, and secondary cleaning treatment is conducted on the outer wall of the breeding box through a spray head and a wiping sponge, so that the transparency of the breeding box is improved, and it is guaranteed that breeding personnel can clearly observe the growth condition of the chlorella in the breeding box; in the feeding mechanism, a rotating motor drives a rotating shaft to rotate, connecting frames evenly distributed on the rotating shaft in the circumferential direction can evenly pump air into the chlorella in the circumferential direction through mutual cooperation with a feeding round pipe, it is guaranteed that the air pumped into the breeding box from top to bottom is evenly distributed, and the growth condition of the chlorella is improved.