Method for quickly accumulating grease through mixotrophic sterile culture of microalgae

Abstract

The invention relates to an algae organism culture technology, in particular to a method for quickly growing chlorella and accumulating a large amount of biological grease through mixotrophic culture. In the method, two-stage culture is adopted, namely at the first stage, high biomass is obtained through the autotrophic aerated culture of the chlorella, and at the second stage, an aerated heterotrophic culture mode of adding organic matters is adopted, the single nutrition mode of the chlorella is changed, and the grease is quickly accumulated on the basis of a large amount of biomass; meanwhile, a novel air sterilization method is adopted to effectively solve the problem of bacterial contamination of culture solution, and culture cost is reduced because a filter membrane is not needed; and algae powder grease is extracted by an alkali-catalyzed anhydrous diethyl ether-petroleum ether extraction method. The method has the advantages that: the whole process is easy to operate, experiment cost and production cost are low, the culture period is short, the grease accumulation concentration of microalgae can be quickly improved, and the extracted grease has high content and purity and is not required to be decolorized; and the method is suitable for preparing microalgae grease in a laboratory or in the industry, and has a good application prospect.

Description

technical field [0001] The invention relates to an algae biological culture technology, in particular to a method for rapidly growing and accumulating biological oil in a large amount by mixing nutrient culture of chlorella. Background technique [0002] In recent years, the global energy demand has continued to expand, and seeking renewable and clean energy that can replace petroleum is an important issue that needs to be solved urgently. Algae have the characteristics of high photosynthetic efficiency, strong environmental adaptability, short growth cycle, high biomass production, and rich oil content, so algae are good materials for preparing bio-oil fuels. But the main problem of producing microalgae biofuel at present is the high cost of cultivation. Only by solving the problem of breeding fast-growing microalgae with high oil content, optimizing culture conditions and processes, improving large-scale production conditions, and improving the efficient separation of tar...

AI Technical Summary

Problems solved by technology

However, the culture medium of heterotrophic growth of algae cells is very easy to be contaminated with bacteria. At present, the ventilation of the fermentation equipment is sterilized by a simple filter device through a filter membrane, which cannot completely remove other bacteria in the air, and the filter membrane needs to be replaced regularly, which is costly.

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