Preparation method of algae biofilm

A technology of algal biofilm and biofilm carrier, which is applied in the field of preparation of algal biofilm, can solve the problems that the details are not clearly described, the research on algal biofilm is few, etc., and achieves good variability, low preparation cost, and good light transmittance. Effect

Inactive Publication Date: 2016-10-12
GUANGXI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Although the above two documents have some explanations on the preparation of algae biofilm and its treatment of sewage, they fail to describe the specific details clearly. At present, there are relatively few domestic researches on algae biofilm.

Method used

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  • Preparation method of algae biofilm
  • Preparation method of algae biofilm

Examples

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Embodiment 1

[0035] A kind of preparation method of algae biofilm, its preparation steps are as follows:

[0036] (1) Carrier pretreatment: Soak the biofilm carrier in 0.05mol / L NaHCO 3 In the solution, soaking time: 25 hours, then rinse with water 3 times, soak the obtained three-dimensional elastic carrier in hydrochloric acid with a concentration of 2.0 mg / L for 11 hours, rinse with deionized water and dry;

[0037] (2) Algae cultivation:

[0038] a. Preparation of BG11 medium mother liquor: Accurately weigh the medicines according to the basic component formula of BG11 medium, dissolve them in deionized water respectively, and measure the corresponding volume according to the dosage when using;

[0039] b. Preparation of medium: Measure the required volume of medium mother liquor, stir evenly, and use 1mol / L NaOH solution to adjust the pH to 7.1 to obtain the medium;

[0040] c. Sterilization of the medium: divide the medium into conical flasks, and the total volume of the medium add...

Embodiment 2

[0050] A kind of preparation method of algae biofilm, its preparation steps are as follows:

[0051] (1) Carrier pretreatment: Soak the biofilm carrier in 0.25mol / L NaHCO 3 In the solution, soaking time: 24.5 hours, then rinse with water 4 times, soak the obtained semi-soft carrier in hydrochloric acid with a concentration of 1.7mg / L for 11.5 hours, rinse with deionized water and dry;

[0052] (2) Algae cultivation:

[0053] a. Preparation of BG11 medium mother liquor: Accurately weigh the medicines according to the basic component formula of BG11 medium, dissolve them in deionized water respectively, and measure the corresponding volume according to the dosage when using;

[0054] b. Preparation of medium: Measure the required volume of medium mother liquor, stir evenly, and use 1mol / L HCl solution to adjust the pH to 6.9 to obtain the medium;

[0055] c. Sterilization of the medium: divide the medium into conical flasks, and the total volume of the medium added to the coni...

Embodiment 3

[0065] A kind of preparation method of algae biofilm, its preparation steps are as follows:

[0066] (1) Carrier pretreatment: Soak the biofilm carrier in 0.4mol / L NaHCO 3 In the solution, soaking time: 24 hours, then rinse with water 5 times, soak the obtained soft carrier in hydrochloric acid with a concentration of 1.4mg / L for 12 hours, rinse with deionized water and dry;

[0067] (2) Algae cultivation:

[0068] a. Preparation of BG11 medium mother liquor: Accurately weigh the medicines according to the basic component formula of BG11 medium, dissolve them in deionized water respectively, and measure the corresponding volume according to the dosage when using;

[0069] b. Preparation of medium: Measure the required volume of medium mother liquor, stir evenly, and use 1mol / L NaOH solution or HCl solution to adjust the pH to 7.1 ± 0.2 to obtain the medium;

[0070]c. Sterilization of the medium: divide the medium into conical flasks, and the total volume of the medium added...

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Abstract

The invention belongs to the technical field of environmental protection, and particularly relates to a preparation method of an algae biofilm and application thereof in sewage treatment. The preparation method specifically comprises the steps of carrier pretreating, algae culturing, algae domesticating and algae biofilm preparing. The preparation method of the algae biofilm has the advantages that the preparation cost is low, the raw materials are easy to obtain, and the algae biomass is easy to recycle; the prepared algae biofilm can achieve the efficient nitrogen and phosphorus removal effect in sewage treatment, achieves the purpose of general sewage treatment and not only can overcome the defects that in a suspended algae system, the algae density is low, the hydraulic retention time is long, algae and water are difficult to separate, too more suspended solids (SS) exist, the resource recycling rate is low but also has the advantages of reducing immobilization reagent cost, weakening the influences of immobilization reagents on the algae activity, reusing immobilization carriers and the like.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a preparation method of algae biofilm and its application in sewage treatment. Background technique [0002] People produce a large amount of domestic sewage in daily life, and these sewage contain a large amount of organic matter rich in nitrogen and phosphorus. Direct discharge into the river will increase the content of nitrogen and phosphorus elements in the river water, and the increase in the content of nitrogen and phosphorus pollutants in the water will cause excessive reproduction of algae and other plankton in the water, increase the content of organic matter in the water, and produce algal toxins , Affect the landscape effect and availability of water, cause the decrease of dissolved oxygen in the water body, the deterioration of water quality, and the phenomenon of eutrophication in the water body, which in turn affects the social and econ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/02C12N11/08C12N1/36C02F3/32C12R1/89
CPCC02F3/322C12N1/36C12N11/02C12N11/08
Inventor 魏群涂晓杰朱宇轩李晓伟陈延飞叶华金锂
Owner GUANGXI UNIV
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