Preparation of algae biological membrane and application of algae biological membrane in urban secondary effluent treatment

A technology of algae biofilm and secondary effluent, applied in the field of water treatment, can solve the problems of difficult harvesting of algae biomass, long hydraulic retention time, low treatment load, etc., and achieve good application prospects, easy cultivation and harvesting, and stable operation.

Pending Publication Date: 2020-11-03
自然资源部天津海水淡化与综合利用研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the suspended algae culture system treats sewage, there are defects such as low algae density, low treatment load, difficult harvest of algal biomass, high suspended solids, and long hydraulic retention time.

Method used

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  • Preparation of algae biological membrane and application of algae biological membrane in urban secondary effluent treatment
  • Preparation of algae biological membrane and application of algae biological membrane in urban secondary effluent treatment
  • Preparation of algae biological membrane and application of algae biological membrane in urban secondary effluent treatment

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preparation example Construction

[0021] A kind of preparation method of algae biofilm of the present invention, comprises the steps:

[0022] (1) Expanded cultivation of microalgae: adding microalgae into a microalgae membrane reactor filled with microalgae culture solution for expanded cultivation to increase the density of algae cells. Expanded culture conditions: under the conditions of light intensity of 3000Lux-4000Lux, light-dark ratio of 12h:12h, and temperature of 23±2°C, aerated culture for 5d-8d, so that the microalgae grow well and quickly reach the logarithmic growth phase; preferred The microalgae were inoculated into the culture solution at an inoculation amount of 5% by volume of the microalgae culture solution.

[0023] Preferably, the microalgae is any one of Spirulina, Scenedesmus, Synechococcus or Chlorella.

[0024] Preferably, when the microalgae is Scenedesmus, Synechococcus or Chlorella, the microalgae culture medium (medium) is BG-11, which is available in the market; when the microal...

Embodiment 1

[0033] (1) Expanded cultivation of microalgae: Prepare 20 L of culture solution and add it to the microalgae membrane reactor. The culture medium of this embodiment is BG-11 medium, NaNO 3 30g, K 2 HPO 4 0.8g, MgSO 4 .7H 2 O 1.5g, CaCl 2 ·7H 2 O 0.72g, Na 2 CO 3 0.4g, citric acid 0.12g, ferric citrate 0.12g, trace element A5 20mL. Transfer 1000mL of Chlorella algae to BG-11 culture medium, under the conditions of light intensity of 3500Lux, light-dark ratio of 12h:12h, and temperature of 23°C, aerated culture for 6 days, so that the growth of Chlorella reaches the logarithmic phase .

[0034](2) Preparation of algae film: fix the biological curtain of polyester fiber in the reactor, immerse in the chlorella culture solution, under the conditions of light intensity 3500Lux, light-dark ratio 12h:12h, temperature 23 ℃, static After culturing for 6 days, the surface of the biofilm carrier is covered with algae, and the film hanging is completed, and the chlorella algae...

Embodiment 2

[0038] (1) Expanded cultivation of microalgae: Prepare 20 L of culture solution and add it to the microalgae membrane reactor. The culture fluid of this embodiment is Zarrouk medium NaHCO 3 336g, NaNO 3 50g, K 2 HPO 4 10g, MgSO 4 ·7H 2 O 4.0g, NaCl 20g, K 2 SO 4 20g, FeSO 4 ·7H 2 O 0.2g, Na 2 EDTA 0.8g, trace element A5 20mL. Transfer 1000mL of Spirulina algae into Zarrouk culture medium, under the conditions of light intensity of 4000Lux, light-dark ratio of 12h:12h, and temperature of 25°C, aerated culture for 5 days, so that the growth of Spirulina reached the logarithmic phase.

[0039] (2) Preparation of algae film: Fix the semi-soft carrier made of polyethylene material in the reactor, immerse in the spirulina culture solution, under the conditions of light intensity 4000Lux, light-dark ratio 12h:12h, temperature 25°C , under static culture for 4 days, until the surface of the biofilm carrier is covered with algae, and the film hanging is completed, and the...

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Abstract

The invention discloses preparation of an algae biological membrane and an application of the algae biological membrane in urban secondary effluent treatment. The preparation method comprises the following steps: (1) performing expanded culture of microalgae; (2) performing biological membrane culturing of the microalgae; and (3) domesticating the algae membrane. Compared with a suspended algae technology, a technology for treating the urban secondary effluent by utilizing the algae biological membrane prepared from autotrophic microalgae has the advantages of simplicity in operation, stability in operation, easiness in culture and harvesting, reusability of a fixed carrier and the like, does not need any immobilized medicament, hardly generates negative effects on the environment, and hasgood application prospects in the sewage treatment.

Description

technical field [0001] The invention belongs to the technical field of water treatment, and in particular relates to a preparation method of algae biofilm and its application in urban secondary effluent treatment. Background technique [0002] At present, my country's urban sewage treatment technologies mainly include activated sludge method and biofilm method. These technologies can remove most of the organic pollutants in sewage, but only 20% to 40% of nutrients such as nitrogen and phosphorus can be removed. The secondary effluent of domestic sewage is easily affected by factors such as influent water quality, organic carbon source, temperature, etc., and it is difficult to achieve efficient nitrogen and phosphorus removal. The effluent contains high concentrations of phosphate, ammonia nitrogen and other pollutants, which may easily cause eutrophication of the water body. [0003] As a readily available autotrophic biological resource, microalgae have the advantages of a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/36C12N11/082C12N11/096C02F3/32C12R1/89
CPCC12N1/12C12N1/36C12N11/082C12N11/096C02F3/322
Inventor 张晓青成玉司晓光曹军瑞邱金泉任华峰
Owner 自然资源部天津海水淡化与综合利用研究所
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