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Culture method for improving microalgae carbon fixing and oil producing capacity

A cultivation method and technology of microalgae, applied in the cultivation field of improving microalgae's carbon fixation and oil production capacity, can solve the problems of inhibiting microalgae growth, achieve the effect of improving carbon fixation and oil production capacity, and increasing tolerance

Active Publication Date: 2014-02-05
博露(厦门)生物股份有限公司
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Problems solved by technology

However, many studies have shown that the continuous supply of high-concentration carbon dioxide inhibits the growth of microalgae

Method used

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  • Culture method for improving microalgae carbon fixing and oil producing capacity
  • Culture method for improving microalgae carbon fixing and oil producing capacity

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Embodiment 1

[0009] Embodiment 1. The specific steps of the cultivation method for improving the carbon fixation and oil production capacity of Nannochloropsis (N.salina) in this embodiment are as follows:

[0010] 1. Microalgae cultivation: Inoculate Nannochloropsis N.salina into three Erlenmeyer flasks containing f / 2 medium, the initial pH is 8, the temperature is 23-27°C, and the light is 65-95 μmol m -2 the s -1 . Nutrient-rich f / 2 medium, containing per liter: 33.6g artificial sea salt, 0.075g NaNO 3 , 0.00565g NaH 2 PO 4 2H 2 O, 1ml trace elements (T) and 1ml vitamins. Among them, every liter of trace element T contains: 4.16g Na 2 EDTA, 3.15g FeCl 3 ·6H 2 O, 0.18g MnCl 2 4H 2 O, 10mg CoCl 2 ·6H 2 O, 10 mg CuSO 4 ·5H 2 O, 22mg ZnSO 4 ·7H 2 O, 6 mg Na 2 MoO 4 2H 2 O. Each liter of vitamins contains: 100mg vitamin B1, 0.5mg vitamin B12 and 0.5mg vitamin H.

[0011] 2. Supply of carbon dioxide: Into the three cultures of Nannochloropsis N.salina with air containing ...

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Abstract

The invention discloses a culture method for improving microalgae carbon fixing and oil producing capacity, and relates to the technical field of algae organism culture. According to different demands of microalgae on inorganic carbon sources in different stages (lag phase, the incipient stage of logarithmic phase and the middle and later periods of the logarithmic phase) of a growth cycle, the culture method gradually increases the supply of carbon dioxide, not only greatly improves the growth rate of the microalgae and the tolerance of carbon dioxide concentration, but also greatly improves the microalgae carbon fixing and oil producing capacity. The carbon fixing amount in of the microalgae cultured in a progressive-increase manner is respectively increased by 15 times and 32% compared with that of the microalgae cultured in continuous high-concentration carbon dioxide of 18-22% and continuous air. The oil contents of the microalgae cultured in the continuous high-concentration carbon dioxide of 18-22%, in the continuous air and in the progressive-increase manner are respectively 29%, 24% and 47%. The oil producing amount of the microalgae cultured in the progressive-increase manner is respectively increased by 7.7 times and 1.6 times compared with that of the microalgae cultured in the continuous high-concentration carbon dioxide of 18-22% and the continuous air.

Description

technical field [0001] The invention relates to the cultivation technology of algae organisms, in particular to a cultivation method for improving the carbon fixation and oil production capacity of microalgae. Background technique [0002] Greenhouse gases, including carbon dioxide, contribute to global warming and climate change. The control of greenhouse gas emissions, especially carbon dioxide emissions, has become one of the global common issues. While China's economy is developing rapidly, the emission of carbon dioxide is also rising rapidly, and the current total emission has reached the world's first. As one of the signatories of the "Kyoto Protocol", although China is not obliged to reduce emissions as a developing country, the control of carbon dioxide emissions is an inescapable responsibility. The vast majority of carbon dioxide emissions come from the combustion of traditional fossil energy, which accounts for more than three-quarters of the total human carbon...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 陈意民
Owner 博露(厦门)生物股份有限公司
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