Trans-interfacial magnetic separation
A magnet and magnetic field technology, applied in the field of cross-interface magnetic separation, can solve the problem of inability to utilize purification and/or separation of target analyte diagnostic and environmental testing solutions
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0127] Exemplary device fabrication and assembly
[0128] Combinations of 3D printed plastics (Protolabs, Maple Plain, MN), laser cut plastic parts, transfer adhesives (e.g., 3M 9472LE), and hydrophobic coatings (e.g., Aculon NanoProof 5.0, San Diego, CA) have been used to Fabricate an exemplary device. A four-chamber box has been 3D printed from plastic resin. The chamber is sealed at one end, allowing buffer addition through the open end ( Figure 4 ).
[0129] Heat press the threaded inserts into the perimeter holes to facilitate assembly. A polycarbonate film (McMaster-Carr 85585K103) was adhesively bonded one side to 3M 9472LE Adhesive Transfer Tape and laser cut into circular atolls. Atolls were concentrically aligned with each of the four chambers and bonded to the cassette surface.
[0130] The plastic frame was laser cut and placed on top of the box surface ( Figure 6 ). Apply Aculon's NanoProof 5.0 to the second polycarbonate film on one side using a cotton s...
Embodiment 2
[0134] Exemplary Device Operation and Testing
[0135] The functionality of the exemplary TIMS cartridge described in Example 1 was tested in the first three chambers with the following three liquids: Lysis Buffer, Wash 1 (mixture of detergent and alcohol) and Wash 2 (alcohol and aqueous) . These fluids are highly wettable due to the very low contact angles that exist on most surfaces. Add the resuspended PMP suspension to chamber 1. The PMPs in chamber 1 were collected onto a hydrophobically treated polycarbonate membrane using a small cylindrical neodymium magnet (K&J Magnetics, Pipersville, PA). Slowly transfer the PMP pellet across the G h and transferred to chamber 2. After a while, the PMPs were recollected again and transferred to chamber 3 ( Figure 10 ). Tests showed that the liquids remained fixed at the outer edges of their respective atolls. Additionally, there is no liquid bridging during PMP movement. Liquid carryover (contamination) is minimized between ...
Embodiment 3
[0137] other design elements
[0138] Experiments with the exemplary device described in Example 1 have revealed alternative designs that can be incorporated into embodiments within the scope of this document as well as improvements that can be incorporated into the next prototype. For example, increased atoll height (e.g., taller frame) prevents liquid pools from rupturing past the atoll; rounded ID edges on the atoll facilitate PMP transfer; removal of 90 °Corner angle reduces PMP loss during collection; et al. When transferring PMPs from one liquid chamber to another, if the droplet is drawn into the gas barrier, then A h should be > the achieved liquid pool height on the hydrophobic transfer surface for a liquid with a contact angle of 180°, i.e. LP h (as worst case). This permanently prevents the liquid from contacting the surface of the cartridge in the event of the aforementioned situation.
[0139]
[0140] in,
[0141] LP h = height of liquid pool on hydropho...
PUM
| Property | Measurement | Unit |
|---|---|---|
| diameter | aaaaa | aaaaa |
| height | aaaaa | aaaaa |
| height | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More