Method for extracting stevioside by employing simulated moving bed chromatography

Abstract

A method for extracting stevioside by employing the simulated moving bed chromatography comprises the following steps of (1) performing leaf treatment; (2) distilling and concentrating crude extract;(3) separating the stevioside from the concentrated crude extract of the stevioside by using the simulated moving bed chromatography; and (4) concentrating extract liquid and raffinate with a film rotary evaporator until no alcohol smell is left, and performing washout with ethanol, and then, performing drying in a vacuum drying oven until weight is constant. According to the method for extractingthe stevioside by employing the simulated moving bed chromatography, the content of total glycosides is higher than 97%, the content of the stevioside A is higher than 70%, and the purification effect is obvious.

Description

technical field [0001] The invention relates to a method for purifying effective components of natural plants, in particular to a method for extracting steviol glycosides by using simulated moving bed chromatography technology. Background technique [0002] Stevia rebaudiana (stevia rebaudiana), also known as stevia ﹑ sugar grass, belongs to Compositae (Comppositae), Stevia genus, and its origin is in subtropical South America. Since 1976, scientific research units such as Nanjing Zhongshan Botanical Garden and Chinese Academy of Agricultural Sciences have successively introduced stevia from Japan for trial planting. Stevia contains 14 kinds of trace elements and 32 kinds of nutrients. It is not metabolized, not accumulated, not carcinogenic, and non-toxic in the body. Its safety has been recognized by international organizations such as FAO and WHO, and the United States. Sugar source, but also a good source of nutrition. [0003] Steviol glycosides, referred to as stevio...

AI Technical Summary

Problems solved by technology

In these processes, the water consumption is large during water soaking and extraction, which requires distilled water concentration and high energy consumption; when the flocculant is used to remove impurities, a large amount of precipitation will be generated after adding flocculant to the leachate, although most impurities can be removed, but it will also be adsorbed. A part of steviol glycosides reduces the product yield and produces a large amount of salt at the same time; column chromatography purification is a key step of the process, in addition to removing a small amount of pigment, tannin and other sugars remaining in the stevioside extract, it can also desalt and other impurities , but the amount of resin and mobile phase is large, the utilization rate of resin is low, and the resin needs to be regenerated. The regeneration cycle of desalination and decolorization resin is generally very long, and a large amount of waste water is generated