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Hermetia illucens composite microbial preparation, preparation method therefor and application of hermetia illucens composite microbial preparation in conversion of bean dregs

A technology of compound microorganisms and black soldier flies, which is applied in the fields of environmental protection and resource utilization, can solve the problems of low utilization rate of bean dregs resources, and achieve the effects of good development value, short processing cycle and high biotransformation rate.

Active Publication Date: 2020-05-19
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Purpose of the invention: the technical problem to be solved by this invention is to provide a black soldier fly compound microbial preparation for the deficiencies of the prior art and the low utilization rate of bean dregs as a resource

Method used

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  • Hermetia illucens composite microbial preparation, preparation method therefor and application of hermetia illucens composite microbial preparation in conversion of bean dregs
  • Hermetia illucens composite microbial preparation, preparation method therefor and application of hermetia illucens composite microbial preparation in conversion of bean dregs
  • Hermetia illucens composite microbial preparation, preparation method therefor and application of hermetia illucens composite microbial preparation in conversion of bean dregs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: a kind of method of joint black soldier fly larva transforms bean dregs, comprises the following steps:

[0043] (1) Three species of bacteria isolated from the gut of the black soldier fly: Enterococcus faecalis CCTCC NO: M 2019612; Proteus mirabilis CCTCC NO: M 2019613; Citrobacter freundii CCTCC NO: M 2019614 were cultured in nutrient broth Base (beef extract 3g / L, peptone 10g / L, sodium chloride 5g / L; pH of the nutrient broth medium is 7.0-7.4) shaker expansion culture, 150r / min, 37 ℃ for 24h;

[0044] (2) Sterilize the concentrated feed in an autoclave at 121° C. for 15 minutes.

[0045] (3) Mix the 3 kinds of bacterial solutions according to the volume ratio of 1:1:1, inoculate 50% (mL / g) of the mixed bacterial solution in the concentrate feed, and mix the 3 kinds of bacterial solutions with the sterilized concentrate feed evenly Then put it in the feed box, put the unhatched eggs on the gauze above the feed box (the ratio of eggs to mixed material i...

Embodiment 2

[0049] Embodiment 2: a kind of method of joint black soldier fly larva transforms bean dregs, comprises the following steps:

[0050] (1) Enterococcus faecalis NO: CCTCC M 2019612 isolated from the intestinal tract of the black soldier fly was expanded and cultured on a nutrient broth medium shaker, 150r / min, 37°C for 24h;

[0051] (2) Sterilize the concentrated feed in an autoclave at 121° C. for 15 minutes.

[0052] (3) Inoculate 50% (mL / g) mixed bacterial solution in the concentrated feed, mix the bacterial solution and the sterilized concentrated feed evenly and place it in the feed box, and put the unhatched eggs (worm eggs and the mixture) Ratio of feed 1g / kg) placed on the gauze above the feed box, hatched at 25-30°C for 4-5 days, after the eggs hatched, fell into the concentrated feed, at 28°C, relative humidity 70%, mixed feed The mass percentage of water in the medium was continued to be raised for 5 days under the condition of 70%, so that the mixed bacterial strai...

Embodiment 3

[0056] Embodiment 3: a kind of method of joint black soldier fly larva transforms bean dregs, comprises the following steps:

[0057] Same as Example 2, only the Enterococcus faecalis NO: CCTCC M 2019612 is changed to Proteus mirabilis CCTCC NO: M2019613.

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Abstract

The invention discloses a hermetia illucens composite microbial preparation, a preparation method therefor and application of the hermetia illucens composite microbial preparation in conversion of bean dregs. The preparation method comprises the steps: inoculating a nutrient broth culture medium with strains separated from intestinal tracts of hermetia illucens for culture, so as to obtain a bacterial suspension, i.e., the composite microbial preparation. The strains separated from the intestinal tracts of the hermetia illucens comprise any one or a combination of enterococcus faecalis, proteus mirabilis and Citrobacter freundii. Compared with the prior art, the hermetia illucens composite microbial preparation has the following advantages that the strains employed are all autochthony screened out from intestinal tracts of hermetia illucens larvae, the strains can be better enriched and implanted into the intestinal tracts of the hermetia illucens again due to the fact that the strainsare autochthony, florae of the intestinal tracts of the hermetia illucens larvae are changed from a source and can better carry out conversion, and the biological conversion ratio reaches up to 17.33%.

Description

technical field [0001] The invention relates to the fields of environmental protection and resource utilization, in particular to a black soldier fly composite microbial preparation, a preparation method thereof and an application in transforming bean dregs. Background technique [0002] Okara is a by-product obtained after soybeans are soaked, peeled, refined, and separated from slag during tofu processing. Since the invention of tofu, tofu has always played an irreplaceable role on people's tables. Each ton of soybean processing produces about 1.2 tons of wet bean dregs. According to incomplete statistics, the average annual production of wet bean dregs in my country is higher than 20 million tons. The huge annual output has caused serious problems for its treatment. According to statistics, there are currently 170 billion tons of biomass resources in the world, and China is carrying nearly one-third of the world's low- and medium-grade biomass emissions with less than 7%...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F3/00C12R1/01C12R1/37
CPCC12N1/20C05F3/00Y02A40/20
Inventor 熊强周燊张靖
Owner NANJING UNIV OF TECH
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