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Method of diagnosis, staging and monitoring of melanoma using microrna gene expression

A melanoma, expression level technology, applied in the field of diagnosis of melanoma

Inactive Publication Date: 2020-05-19
GENESEQ PTY LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Therefore, there is an urgent unmet need for objective methods to detect the presence of viable, malignant melanoma cells using non-invasive, low-cost, high-throughput methods

Method used

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  • Method of diagnosis, staging and monitoring of melanoma using microrna gene expression
  • Method of diagnosis, staging and monitoring of melanoma using microrna gene expression
  • Method of diagnosis, staging and monitoring of melanoma using microrna gene expression

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Embodiment Construction

[0210] General techniques and selected definitions

[0211] Unless expressly defined otherwise, all technical and scientific terms used herein should be regarded as having terms commonly used in the art (e.g., molecular biology, cell culture, immunology, immunohistochemistry, protein chemistry, and biochemistry). The same meaning as commonly understood by a skilled person.

[0212]Unless otherwise indicated, the techniques employed in the present invention are standard procedures well known to those skilled in the art. Such techniques are described and illustrated throughout in the following literature resources, for example J. Perbal, A Practical Guide to Molecular Cloning, John Wiley and Sons (1984); J. Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press (1989); T.A. Brown (editor), Essential Molecular Biology: A Practical Approach, Volumes 1 and 2, IRL Press (1991); D.M. Glover and B.D. Homes (editors), DNA Cloning: A Practical Appr...

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Abstract

The present invention relates to methods of diagnosing melanoma in a subject, the method comprising detecting microRNA expression levels. The present invention also relates to methods of assessing thedisease stage of melanoma and / or monitoring said melanoma stage. Also, the present invention relates to selecting a treatment or modifying a treatment based on the diagnosis or stage of melanoma. Further the present invention relates to a system for detecting and diagnosing melanoma in a patient and for determining the disease stage of melanoma.

Description

technical field [0001] The present invention relates to a method of diagnosing melanoma in a subject, said method comprising detecting microRNA (microRNA) expression levels. The invention also relates to methods of assessing the disease stage of melanoma and / or monitoring said melanoma stage. Additionally, the invention relates to selecting or adjusting treatment based on the diagnosis or stage of melanoma. The invention also relates to a system for detecting and diagnosing melanoma in a patient and for determining the disease stage of melanoma. Background technique [0002] Globally, more than 130,000 people are diagnosed with melanoma each year. Although melanoma is the least common form of skin cancer, it is also the deadliest. Over the past 10 years, incidence has increased proportionally to depletion of atmospheric ozone levels and solar UV radiation. Risk factors for developing melanoma include fair skin, freckles, and light hair; recreational and occupational sun ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574G16B25/10
CPCC12Q1/6886C12Q2600/112C12Q2600/178G01N2800/7028C12Q2600/158
Inventor 瑞安·万拉尔
Owner GENESEQ PTY LTD
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