Novel coronavirus multiplex PCR rapid detection kit

Abstract

The invention discloses a novel coronavirus multiplex PCR rapid detection kit. The novel coronavirus multiplex PCR rapid detection kit comprises PCR reaction liquid freeze-dried powder, primer pool freeze-dried powder and a buffer solution, wherein the primer pool freeze-dried powder comprises a primer pair and a fluorescent probe which are listed in a sequence table. Primer system optimization isperformed for detecting SARS-CoV-2 through multiplex PCR, brand-new primers and a brand-new probe pool are designed, and early rapid detection on the new coronavirus can be sensitively and specifically achieved clinically.

Description

technical field [0001] The invention belongs to the technical field of viral nucleic acid detection, in particular to a novel coronavirus multiplex PCR rapid detection kit. Background technique [0002] The clinical manifestations of pneumonia patients infected by the new coronavirus are: fever, fatigue, and dry cough as the main manifestations. Upper respiratory symptoms such as nasal congestion and runny nose are rare. About half of the patients developed dyspnea more than a week later, and severe cases rapidly progressed to acute respiratory distress syndrome, septic shock, metabolic acidosis that was difficult to correct, and coagulation dysfunction. It is worth noting that severe and critically ill patients may have moderate to low fever or even no obvious fever during the course of the disease. Some patients have mild onset symptoms, may have no fever, and mostly recover after 1 week. Most patients have a good prognosis, while a few patients are in critical conditio...

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the technical problem that a single primer pair and probe in the prior art are not clinically sensitive and cannot detect the new coronavirus early and quickly, and the purpose is to provide a novel coronavirus multiplex PCR rapid detection kit

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