The present invention relates to a
fluorescence labeled composite amplification detection kit for simultaneously analyzing 17
gene loci of canine
genomic DNA. With the
system provided by the kit, genetic polymorphisms of the 17
gene loci of the canine
genomic DNA can be simultaneously analyzed. The 17
gene loci of the canine
genomic DNA comprise DAmel, PEZ1, PEZ2, PEZ3, PEZ5, PEZ6, PEZ8, PEZ12, PEZ15, PEZ20, PEZ21, FH2010, FH2054, FH2079, FH2132, FH2611 and VWFX, is divided into four groups, and relates to the
fluorescence labels comprising five colors. The
fluorescence labeled composite amplification detection kit provided by the present invention is provided for composite amplification of a mixture comprising
oligonucleotide primers of the 17 gene loci. In the
system, the gene locus foridentifying the gender of the
DNA samples is the DAmel gene locus. The detection results of the
system has characteristics of high polymorphism, good balance, high sensitivity, strong specificity, accurate
typing result, strong species-specificity, and can fully meet the requirements of dog individual identification, paternity identification, and
DNA database construction.