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Application of pilose antler protein extract in preparation of Parkinson's disease (PD) treating drug

A protein extract and deer antler technology, applied in the field of protein extraction, can solve problems such as toxicity system research

Pending Publication Date: 2020-06-09
EXPERIMENTAL RES CENT CHINA ACAD OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, while the existing research has affirmed the effect of deer antler in repairing nerve damage, it has not systematically studied its toxicity.

Method used

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  • Application of pilose antler protein extract in preparation of Parkinson's disease (PD) treating drug
  • Application of pilose antler protein extract in preparation of Parkinson's disease (PD) treating drug
  • Application of pilose antler protein extract in preparation of Parkinson's disease (PD) treating drug

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of working solution:

[0031] ①Configuration of buffer solution:

[0032] Buffer A (Tris-HCl, 20mmol / L): Take Tris (trishydroxymethylaminomethane) 2.422g in ddH 2 In O, use 1mol / l HCl to adjust pH=8.5 and make the volume in a 1L volumetric flask.

[0033] BufferB (Tris-HCl-NaCl, 1mol / L): Take the prepared Tris-HCl buffer solution 500ml and add 29.22g NaCl.

[0034] ②ddH 2 O: Used to replace the buffer in the pipeline to prevent salt precipitation in the buffer and block the column.

[0035] ③20% ethanol: When eluting with water for 2-3 column volumes, it can be used to fill the column to prevent bacterial growth.

[0036] ④Ultrafilter the above working solution through a 0.22μm filter membrane, and sonicate for about 10 minutes to remove air bubbles.

[0037] 1) The preparation method of pilose antler protein extract, concrete steps are as follows:

[0038] (1) Fresh velvet antler is directly quick-frozen with liquid nitrogen, placed in a superfine pul...

Embodiment 2

[0053] 1) Nematode culture

[0054] Spread OP50 Escherichia coli evenly on NGM agar plates and culture overnight at 37°C. Transfer Caenorhabditis elegans A30P to a new NGM agar plate containing bacteria every 2 days, and culture at a constant temperature in a 20°C incubator.

[0055] 2) Nematode synchronization

[0056] Gently wash the A30P nematodes at the oviposition stage with M9 from the NGM plate, collect them in a 1.5mL centrifuge tube; after standing still, centrifuge at 4000g for 1min, discard the supernatant; add an appropriate amount of M9, wash repeatedly 3 times; add 1mL nematode lysate , shake and mix for 1 min, centrifuge to remove the supernatant; add M9 to wash repeatedly for 3-5 times, then add to a blank NGM plate and incubate overnight to obtain synchronized L1 stage A30P nematodes.

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Abstract

The invention discloses application of a pilose antler protein extract in preparation of a Parkinson's disease (PD) treating drug and belongs to the technical field of protein extraction. According tothe application of the pilose antler protein extract in preparation of the PD treating drug, a Parkinson model nematode is used as a research object, the pilose antler protein extract is determined to recover behaviors of A30P nematode and inhibit dendrite degeneration deficiency and effectively treat the PD of the A30P nematode in a concentration range of 1-4 mg / mL, and a research basis is provided for treatment of the PD by the pilose antler protein extract later.

Description

technical field [0001] The invention relates to the technical field of protein extraction, in particular to the application of velvet antler protein extract in the preparation of medicines for treating Parkinson's disease. Background technique [0002] Parkinson's Disease (PD) is the second largest neurodegenerative disease after Alzheimer's disease. Epidemiological studies have shown that PD patients account for 1-2% of people over 65 years old worldwide. %, accounting for 4 to 5% of people over the age of 85. PD not only seriously affects the physical and mental health of patients, but also increases the economic burden on families and society. Modern studies believe that the loss of dopamine neurons in the substantia nigra of the brain at the onset of PD leads to a decrease in the secretion of dopamine in the body, followed by a series of motor dysfunction such as tremor, slowness of movement, and muscle stiffness. At present, most of the drugs recommended by clinical g...

Claims

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Application Information

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IPC IPC(8): A61K38/01A61K35/32A61P25/16A61P33/10C07K1/14
CPCA61K38/012A61K35/32A61P25/16A61P33/10C07K1/145
Inventor 王毅孙娅楠赵鑫
Owner EXPERIMENTAL RES CENT CHINA ACAD OF CHINESE MEDICAL SCI
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