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A multi-organ chip based on microfluidic technology and its application
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A microfluidic technology and multi-organ technology, which is applied in the application field of multi-organ chip in organ co-culture and disease model construction, can solve the problems of organ co-culture and inability to be cultured alone, and achieve increased activity, increased concentration, and improved The effect of proportion
Active Publication Date: 2022-07-15
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology
[0004] Existing multi-organ chips can only achieve co-cultivation of organs and cannot be cultured alone
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Embodiment 1
[0039] Liver / pancreas / fat drug metabolism model
[0040] The prepared four-layer structure of the liquid pool layer 1, the micropit array layer 2, the porous elastic membrane layer 3 and the liquid channel layer 4 is irreversibly sealed by oxygen plasma.
[0041] The multi-organ chip was sterilized by high temperature and high pressure, and dried at 80°C for 2h. Hepg2 (liver cells), TC6 (islet cells) and primary adipocytes were digested into single cells and seeded in different pools. After 12h, the cells became spherical. The liquid path is air at this time, no medium is added, and the organs are cultured separately. Among them, streptozotocin was added to the medium of islets, which caused damage to the islets, and established a type 1 diabetes model.
[0042] At this time, the medium is added from the medium input hole 1a, the liquid channel is filled with liquid, the surface tension of the liquid at the microwell array is broken, and the material exchange between the li...
Embodiment 2
[0045] Liver / gut / heart drug metabolism model
[0046] The prepared four-layer structure of the liquid pool layer 1, the micropit array layer 2, the porous elastic membrane layer 3 and the liquid channel layer 4 is irreversibly sealed by oxygen plasma.
[0047]The multi-organ chip was sterilized by high temperature and high pressure, and dried at 80°C for 2h. Hepg2 (liver cells), caco2 (intestinal epithelial cells), and ips-cardiocytes (cardiomyocytes) were digested into single cells and inoculated in different liquid pools respectively, and the cells became spherical after 12 hours. The liquid path is air at this time, no culture is added, and the organs are cultured separately. Among them, the medium of liver and intestine was replaced normally, and the medium of heart was not replaced. After 3 days, the beating of the heart decreased significantly, and it became a pathological state.
[0048] At this time, the medium is added from the medium input hole 1a, the liquid chann...
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Abstract
The invention discloses a multi-organ chip based on microfluidic technology and its application. The organ chip consists of four layers including a liquid pool layer, a micropit array layer, a porous elastic membrane layer and a liquid channel layer from top to bottom, which are irreversibly sealed by oxygen plasma. The organ chip can realize both the separate culture of different organs and the co-culture of different organs. When multiple organs need to be cultured separately, the liquid channel layer is not injected with liquid, and the culture medium of each organ module will not communicate due to the effect of surface tension at the micropores. When two organs are required to interact, the surface tension can be broken by injecting fluid into the fluid path and multiple organs can be connected. The gas is then used to realize the function of an online peristaltic pump, so that the liquid flows directionally, and the perfusion culture and interaction of the two organs are realized. The invention overcomes the shortcomings of traditional cell culture, can independently culture three organs in the initial stage, and can also realize perfusion culture, which is beneficial to material exchange and maintenance of cell activity.
Description
technical field [0001] The invention relates to the application field of multi-organ chips in organ co-culture and disease model construction, in particular to a multi-organ chip based on microfluidic technology and its application. Background technique [0002] Billions of dollars are now wasted around the world every year on the screening of drugs that ultimately fail. Currently, the commonly used drug screening models include cell models and animal models. Animal models are species different from humans and cannot predict drug efficacy well. Cell models are often a single type of cell and are cultured in two dimensions, which cannot reproduce the interactions between organs. [0003] Interaction between cells (including organs) is a fundamental feature of multicellular organisms. Cell-to-cell interactions can occur through direct contact, such as between different cells in a tissue; cell-to-cell interactions can also occur indirectly, such as cell-to-cell interactions ...
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