Nano-ultrasonic microbubble, as well as preparation method and application thereof

A technology of ultrasonic microbubbles and nanometers, which is applied in echo/ultrasonic imaging agents, pharmaceutical formulations, preparation of X-ray contrast agents, etc. It can solve the problems of ultrasonic microbubble imaging and poor targeting, and achieve enhanced therapeutic effects and inhibition Oncogene expression and effect enhancement effect

Inactive Publication Date: 2020-06-19
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, because the microbubbles are at the nanometer level and carry the gene therapy drug CD44 antibody on the surface, their targeting for tumor treatment is greatly enhanced, which can effectively solve the problem of poor imaging and targeting of ultrasonic microbubbles in sonodynamic therapy.

Method used

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  • Nano-ultrasonic microbubble, as well as preparation method and application thereof
  • Nano-ultrasonic microbubble, as well as preparation method and application thereof
  • Nano-ultrasonic microbubble, as well as preparation method and application thereof

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Embodiment 1

[0040] Step 1. Mix distearoylphosphatidylethanolamine-polyethylene glycol (mPEG2000-DSPE) and phosphatidylethanolamine-polyethylene glycol 2000-maleimide (Mal-PEG2000-DSPE) in a molar ratio of 4:1 Dissolve in chloroform, vacuum rotary evaporation at 42°C and 120 rpm to form a film until the chloroform is completely removed, add phosphate buffer saline (PBS), 60°C, rotary hydration at 60 rpm for 1 hour to obtain a micellar solution;

[0041] Step 2. Mix the nano-CD44 antibody with phosphatidylethanolamine-polyethylene glycol 2000-maleimide in the micellar solution obtained in step 1 at a molar ratio of 1:50 in phosphate buffer, and incubate overnight at 4°C to obtain CD44-PEG2000-DSPE;

[0042] Step 3. Dipalmitoylphosphatidylcholine (DPPC) and CD44-PEG2000-DSPE, cholesterol and curcumin are dissolved in a mixed solution of 3mL chloroform and 6mL methanol with a molar ratio of 9:1:0.45:1.6;

[0043] Step 4. Use a rotary evaporator to remove the organic solvent by vacuum rotary ...

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Abstract

The invention discloses a nano-ultrasonic microbubble, as well as a preparation method and application thereof. The nano-ultrasonic microbubble has a shell-kernel structure, wherein the inner kernel is liquid fluorocarbon nano-particles and curcumin, and the shell is a lipid film; and the surface of the lipid film is coupled with CD44 antibody. The nano-ultrasonic microbubble has good imaging, canbe used as an ultrasonic developing contrast agent, a CT contrast agent and an MRI contrast medium, is a multifunctional contrast medium and shows specific superiority in the aspect of targeting development. The nano-ultrasonic microbubble can achieve transformation from nano-grade to micron-grade after accepting ultrasonic excitation, and can release coated drugs to improve the curative effect of sonodynamic treatment. Due to the common effects of nano-grade particle size, sound-sensitive agent curcumin and CD44 antibody, the nano-ultrasonic microbubble achieves excellent target property andtreatment effect in tumor treatment and can be used for treating ovarian cancer, cervical cancer, lung cancer, colorectal cancer, breast cancer and esophagus cancer.

Description

technical field [0001] The invention belongs to the technical field of medical preparations, and in particular relates to a nano-ultrasonic microbubble and its preparation method and application. Background technique [0002] Ovarian cancer is one of the three major malignant tumors of the female reproductive system, and its mortality rate ranks first among gynecological malignant tumors. Ovarian cancer patients have no obvious symptoms and signs in the early stage, so 75% of the patients are already in the advanced stage when they are diagnosed. Currently, the main treatments for ovarian cancer include cytoreductive surgery and cisplatin-based combined chemotherapy. However, the complete clinical remission rate of patients with stage III and stage IV ovarian cancer with chemotherapy after tumor debulking is only 50% to 80%. Still relapse. This phenomenon has been well explained after the theory of tumor stem cells was put forward. Since the current conventional chemother...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22A61K49/04A61K49/18A61K41/00A61K31/12A61P35/00
CPCA61K49/0002A61K49/223A61K49/04A61K49/1812A61K41/0033A61K31/12A61P35/00
Inventor 王曦辉袁春燕唐秋莎王小兰
Owner SOUTHEAST UNIV
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