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A kind of cultivation method of Passiflora new variety

A cultivation method and technology of Passiflora, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of slow progress in induction of Passiflora polyploidy, and achieve the effect of a simple method

Active Publication Date: 2022-03-11
SOUTHWEST FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the defect that the above-mentioned prior art exists, provide a kind of method by endosperm induction, differentiation, directly form triploid regeneration plant, need not purify, solve the existence of Passiflora polyploid induction in the prior art slower progress issues

Method used

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  • A kind of cultivation method of Passiflora new variety
  • A kind of cultivation method of Passiflora new variety
  • A kind of cultivation method of Passiflora new variety

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Embodiment

[0042] Present embodiment provides a kind of breeding method of Passiflora new variety, and this method comprises the following steps:

[0043] 1. Seed treatment: screen out the plump Gypsophila seeds and soak them in 80% concentrated sulfuric acid for 3.5 hours, then wash the passionflower seeds soaked in concentrated sulfuric acid, and scrape off the softened seeds with concentrated sulfuric acid with a knife outer testa. Disinfect the seeds with 75% alcohol for 20 seconds, then disinfect them with 0.1% mercuric chloride for 8 minutes, and then put the seeds on the aseptic operating table. Shake them constantly during the process to fully disinfect the seeds and reduce the contamination of the seeds. degree. After the seeds are sterilized, they are rinsed 4 times with sterile distilled water prepared in advance, and then the seeds are peeled off and the endosperm is cut.

[0044] 2. Endosperm callus induction: the medium is MS medium (MS+30g / L sucrose+3.7g / L agar+0.5mg / lTD...

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Abstract

The invention provides a method for cultivating a new variety of passionflower, which uses the variety of passionflower Gypsophila to carry out endosperm culture to finally obtain triploid plants. The present invention successfully obtains a batch of triploid plants by culturing the endosperm of the Gypsophila variety of Passiflora gypsophila, and utilizes this breeding method to cultivate new seedless or seedless Passiflora suitable for processing and eating. Variety.

Description

technical field [0001] The invention relates to the technical field of polyploid breeding, in particular to a method for cultivating a new variety of passionflower. Background technique [0002] Sexual breeding has become one of the most widely used methods by breeders at present. It can be used as a carrier for the transfer of genetic material, and can achieve the purpose of no nucleus or less nucleus and overcome self-incompatibility. It is suitable for processing and eating. The excellent new varieties of passionflower with no seeds or few seeds play an important role in the development of passionflower. However, the current research on polyploidy of Passiflora can not meet the needs of production development. Traditional polyploid breeding mainly relies on colchicine, but colchicine induces polyploids to obtain less homozygous tetraploids and more chimeras, and it takes a long time to undergo continuous purification and other methods. Contents of the invention [000...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01H1/08
CPCA01H4/008A01H4/001A01H1/08
Inventor 蔡年辉唐军荣许玉兰孙琪赵李姗王亚楠张焕朱雅静
Owner SOUTHWEST FORESTRY UNIVERSITY
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