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Method for preparing coptis chinensis franch radix fermented enzymatic composition, as well as preparation and application of coptis chinensis franch radix fermented enzymatic composition

A technology of fermented enzymes and complex agents, applied in the fields of application, medical preparations containing active ingredients, anti-inflammatory agents, etc., can solve the problem that the efficacy of the medicine cannot be maximized, so as to improve animal immunity and reduce fermentation costs , the effect of shortening the fermentation cycle

Inactive Publication Date: 2020-07-03
BEIJING GINTEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The use of A. villiensis is only limited to compatibility with other medicinal materials or directly as a veterinary drug or feed additive. The preparation method is simple, and the drug effect cannot be maximized. The further development of A. viridis has significant social and economic benefits

Method used

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  • Method for preparing coptis chinensis franch radix fermented enzymatic composition, as well as preparation and application of coptis chinensis franch radix fermented enzymatic composition

Examples

Experimental program
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Effect test

Embodiment 1

[0056] A preparation method of the fermented enzymatic hydrolysis compound of Weilianxu includes the following steps:

[0057] S1: Inoculate lactic acid bacteria strains into 50mLMRS liquid culture medium for expansion culture, air-bath shaker 200rpm, temperature 35℃, fermentation for 16h, and then insert it into the same liquid medium with 3% inoculum. Expand culture under the same conditions to obtain lactic acid bacteria liquid;

[0058] Inoculate the bacillus strains into 50mL LB culture medium for expansion culture, incubate at 200rpm in an air bath at 35°C for 24h; then insert it into the same liquid culture medium with 3% of the inoculum, and expand under the same conditions Cultivate to obtain bacillus liquid;

[0059] The Aspergillus niger was inoculated into 50 mL of liquid medium prepared by adding 8% by mass of tempeh extract and 5% by mass of defatted soybean powder into the aqueous solution, and continuously fermented at 28°C for 70 hours, and then inoculated with 3% ...

Embodiment 2

[0065] A preparation method of the fermented enzymatic hydrolysis compound of Weilianxu includes the following steps:

[0066] S1: Inoculate lactic acid bacteria strains into 50mLMRS liquid culture medium for expansion culture, air-bath shaker 200rpm, temperature 37℃, fermentation for 20h, and then insert them into the same liquid medium with 3% inoculum. Expand culture under the same conditions to obtain lactic acid bacteria liquid;

[0067] Inoculate the bacillus strains into 50mL LB culture medium for expansion culture, incubate at 200rpm, 37℃, 36h in air bath; then insert it into the same liquid culture medium with 3% inoculum, and expand under the same conditions Cultivate to obtain bacillus liquid;

[0068] The Aspergillus niger was inoculated into 50 mL of liquid culture medium prepared by adding 8% tempeh extract and 5% defatted soybean powder into the aqueous solution, and continuously fermented for 72 hours at 28°C, and then inoculated it with 3% The amount is connected t...

Embodiment 3

[0074] A preparation method of the fermented enzymatic hydrolysis compound of Weilianxu includes the following steps:

[0075] S1: Inoculate lactic acid bacteria strains into 50mLMRS liquid culture medium for expansion culture, air-bath shaker 200rpm, temperature 40℃, fermentation for 24h, and then insert it into the same liquid medium with 3% inoculum. Expand culture under the same conditions to obtain lactic acid bacteria liquid;

[0076] Inoculate the bacillus strains into 50mL LB broth for expansion culture, incubate at 200rpm in an air bath at 40℃ for 48h; then insert it into the same liquid culture medium at 3% of the inoculum, and expand under the same conditions Cultivate to obtain bacillus liquid;

[0077] The Aspergillus niger was inoculated into 50 mL of liquid medium prepared by adding 8% by mass of fermented soybean extract and 5% by mass of defatted soybean powder into the aqueous solution, and continuously fermented at 28°C for 75h, and then inoculated it with 3% The...

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Abstract

The invention discloses a method for preparing a of coptis chinensis franch radix fermented enzymatic composition, as well as a preparation and application of the of coptis chinensis franch radix fermented enzymatic composition. The preparation method comprises the following steps: preparing a compound bacterial preparation consisting of lactobacillus, bacillus and aspergillus niger; and fermenting traditional Chinese medicinal herbs, adding a proper enzymatic preparation, performing fermentation and enzymolysis, and performing supercritical extraction. The invention further provides a powdered preparation preparaed from the composition which is prepared by adopting the preparation method and application of the powdered preparation in preparation of medicines for improving anti-inflammatory reaction and enhancing animal immunity. The powdered preparation can be used for promoting growth of animals, preventing intestinal diseases and eliminating antibiotic residues instead of antibiotics with the advantages of environmental friendliness, safety and high efficiency, can ensure animal product safety and human body health, promotes industrial upgrading of feed industry and breeding industry and has an important meaning for promoting the international competitiveness of livestock products in China.

Description

Technical field [0001] The present invention relates to the field of biotechnology, in particular to a preparation method of a mirinia fermented enzymatic hydrolysis compound and its preparation and application. Background technique [0002] Mirian, one of the varieties of Chinese traditional Chinese herbal medicine Coptis, contains a variety of biologically active substances, which can be anti-bacterial, anti-inflammatory, enhance immunity, improve the ability of animals to resist stress and disease, and improve animal production performance; it also has It is not easy to produce bacterial drug resistance, low toxicity, no residue or low residue. [0003] At present, the medicinal materials of Weilian are becoming more and more tense, and the search for alternatives to traditional Chinese medicine is getting more and more attention. If the roots of Weilian can be fully utilized, not only can the existing resources be effectively used, but also production costs can be reduced and e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/30A23K10/12A23K10/14A23K20/24A23K20/20A61K36/718A61K9/14A61K47/02A61P29/00A61P37/04
CPCA23K10/12A23K10/14A23K10/30A23K20/24A23K20/30A61K9/143A61K36/488A61K36/634A61K36/718A61K2236/19A61K2236/37A61K2236/51A61P29/00A61P37/04A61K2300/00
Inventor 张世新李学刚陈波万宗敏贾付从
Owner BEIJING GINTEN BIOTECH
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