Microbial agent for treating aquaculture wastewater, method for preparing same and application
A microbial inoculum and aquaculture wastewater technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of not considering the total nitrogen index, increasing the burden on farmers, and less involving the removal of total nitrogen, etc. Ease of storage and transportation, increase the number of viable bacteria, and reduce costs
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preparation example Construction
[0051] Another object of the present invention is to provide a kind of preparation method for the microbial inoculum agent that is used to process aquaculture wastewater, comprises the following steps:
[0052] (11) Activate the different strains kept in the refrigerator on the nutrient broth solid medium respectively, pick single bacterium colonies and inoculate them in different containers containing nutrient broth liquid medium, and cultivate to obtain the seed liquid;
[0053] (12) each seed liquid of step (11) gained is inoculated in fermenter respectively, carries out aerobic fermentation, obtains fermented liquid;
[0054] (13) centrifuging each fermented liquid obtained in step (12) to obtain thallus, mixing different thallines and protective agents for vacuum freeze-drying to obtain solid microbial inoculum;
[0055] The bacterial species include bacillus, yeast, nitrifying bacteria and aerobic denitrifying bacteria, and the aerobic denitrifying bacteria include Pseud...
Embodiment 1
[0072] A method for preparing a microbial bacterial agent for treating aquaculture wastewater, comprising the following steps:
[0073] (11) Activate the different strains kept in the refrigerator on the nutrient broth solid medium respectively, pick single bacterium colonies and inoculate them in different containers containing nutrient broth liquid medium, and cultivate to obtain the seed liquid;
[0074] (12) Inoculate each seed liquid obtained in step (11) into a fermenter respectively, carry out aerobic fermentation, and obtain a fermented liquid, wherein, the number of effective viable bacteria in the fermented liquid is greater than 2×10 9 CFU / g;
[0075] (13) centrifuging each fermented liquid obtained in step (12) to obtain thallus, mixing different thallines and protective agents for vacuum freeze-drying to obtain solid microbial inoculum;
[0076] The strains include 5 parts of bacillus, 3 parts of saccharomyces, 4 parts of nitrifying bacteria and 3 parts of aerobi...
Embodiment 2
[0084] A method for preparing a microbial bacterial agent for treating aquaculture wastewater, comprising the following steps:
[0085] (11) Activate the different strains kept in the refrigerator on the nutrient broth solid medium respectively, pick single bacterium colonies and inoculate them in different containers containing nutrient broth liquid medium, and cultivate to obtain the seed liquid;
[0086] (12) Inoculate each seed liquid obtained in step (11) into a fermenter respectively, carry out aerobic fermentation, and obtain a fermented liquid, wherein, the number of effective viable bacteria in the fermented liquid is greater than 2×10 9 CFU / g;
[0087] (13) centrifuging each fermented liquid obtained in step (12) to obtain thallus, mixing different thallines and protective agents for vacuum freeze-drying to obtain solid microbial inoculum;
[0088] The strains include 2 parts of bacillus, 2 parts of saccharomyces, 1 part of nitrifying bacteria and 1 part of aerobic ...
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