Microbial agent for treating aquaculture wastewater, method for preparing same and application

A microbial inoculum and aquaculture wastewater technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of not considering the total nitrogen index, increasing the burden on farmers, and less involving the removal of total nitrogen, etc. Ease of storage and transportation, increase the number of viable bacteria, and reduce costs

Pending Publication Date: 2019-06-14
碧沃丰生物科技(广东)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, nitrogen removal in the treatment of aquaculture wastewater still stays at the level of ammonia nitrogen removal. At present, the existing technologies rarely involve the removal of total nitrogen, including the implementation of the "Discharge Standards for Pollutants in Livestock and Poultry Breeding Industry" (GB18596-20012003-01-01) that is being implemented in my country. ) also does not limit the discharge standard of total nitrogen
After the ammonia nitrogen is converted into nitrite nitrogen and nitrate nitrogen, the traditional process requires an anaerobic environment to achieve denitrification and convert nitrate nitrogen into nitrogen gas. This requires the farmer to add an anaerobic pond, which increases the burden on the farmer. burden, so the existing technology often does not consider the total nitrogen index, and the discharge of sewage with high concentration of total nitrogen into the natural water body will undoubtedly increase the self-purification burden of the natural water body and make the water body eutrophic

Method used

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Examples

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preparation example Construction

[0051] Another object of the present invention is to provide a kind of preparation method for the microbial inoculum agent that is used to process aquaculture wastewater, comprises the following steps:

[0052] (11) Activate the different strains kept in the refrigerator on the nutrient broth solid medium respectively, pick single bacterium colonies and inoculate them in different containers containing nutrient broth liquid medium, and cultivate to obtain the seed liquid;

[0053] (12) each seed liquid of step (11) gained is inoculated in fermenter respectively, carries out aerobic fermentation, obtains fermented liquid;

[0054] (13) centrifuging each fermented liquid obtained in step (12) to obtain thallus, mixing different thallines and protective agents for vacuum freeze-drying to obtain solid microbial inoculum;

[0055] The bacterial species include bacillus, yeast, nitrifying bacteria and aerobic denitrifying bacteria, and the aerobic denitrifying bacteria include Pseud...

Embodiment 1

[0072] A method for preparing a microbial bacterial agent for treating aquaculture wastewater, comprising the following steps:

[0073] (11) Activate the different strains kept in the refrigerator on the nutrient broth solid medium respectively, pick single bacterium colonies and inoculate them in different containers containing nutrient broth liquid medium, and cultivate to obtain the seed liquid;

[0074] (12) Inoculate each seed liquid obtained in step (11) into a fermenter respectively, carry out aerobic fermentation, and obtain a fermented liquid, wherein, the number of effective viable bacteria in the fermented liquid is greater than 2×10 9 CFU / g;

[0075] (13) centrifuging each fermented liquid obtained in step (12) to obtain thallus, mixing different thallines and protective agents for vacuum freeze-drying to obtain solid microbial inoculum;

[0076] The strains include 5 parts of bacillus, 3 parts of saccharomyces, 4 parts of nitrifying bacteria and 3 parts of aerobi...

Embodiment 2

[0084] A method for preparing a microbial bacterial agent for treating aquaculture wastewater, comprising the following steps:

[0085] (11) Activate the different strains kept in the refrigerator on the nutrient broth solid medium respectively, pick single bacterium colonies and inoculate them in different containers containing nutrient broth liquid medium, and cultivate to obtain the seed liquid;

[0086] (12) Inoculate each seed liquid obtained in step (11) into a fermenter respectively, carry out aerobic fermentation, and obtain a fermented liquid, wherein, the number of effective viable bacteria in the fermented liquid is greater than 2×10 9 CFU / g;

[0087] (13) centrifuging each fermented liquid obtained in step (12) to obtain thallus, mixing different thallines and protective agents for vacuum freeze-drying to obtain solid microbial inoculum;

[0088] The strains include 2 parts of bacillus, 2 parts of saccharomyces, 1 part of nitrifying bacteria and 1 part of aerobic ...

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PUM

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Abstract

The invention discloses a microbial agent for treating aquaculture wastewater. The microbial agent comprises bacillus, yeast, nitrifying bacteria and aerobic denitrifying bacteria. The total effectiveviable cell count of the microbial agent is more than 2*10<8> CFU/g. The aerobic denitrifying bacteria include pseudomonas mendocina, pseudomonas stutzeri and alcaligenes faecalis. Accordingly, the invention also provides a method for preparing the microbial agent for treating aquaculture wastewater and application. According to the microbial agent, through mutual cooperation of the bacillus, theyeast, the nitrifying bacteria and the aerobic denitrifying bacteria, COD, BOD5, ammonia nitrogen and total nitrogen in aquaculture wastewater are effectively removed, so that treated outlet water reaches the national urban sewage discharge standard level A standard.

Description

technical field [0001] The invention relates to the technical field of wastewater treatment, in particular to a microbial agent for treating aquaculture wastewater, a preparation method and application thereof. Background technique [0002] With the improvement of people's quality of life, my country's aquaculture industry continues to develop. There are more and more farmers, and the scale of aquaculture is getting larger and larger. The aquaculture industry has always been a major polluter. Due to the poor absorption of protein in the feed by livestock and poultry, more than half of the nitrogen and phosphorus substances in the feed are excreted with feces, so the aquaculture wastewater contains a large amount of nitrogen and phosphorus. If the aquaculture wastewater is directly discharged into the natural water body without treatment, it will cause eutrophication of the water body, which will lead to a series of environmental problems such as cyanobacteria bloom and soil ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/04C02F3/34C12R1/07C12R1/38C12R1/05C12R1/125C12R1/10C12R1/72C12R1/01C02F103/20
CPCC12N1/20C12N1/16C12N1/04C02F3/341C02F3/347C12R2001/38C12R2001/05C12R2001/125C12R2001/07C12R2001/10C12R2001/72C12R2001/01C02F2103/20Y02W10/10
Inventor 胡亚冬黎伟杰夏雨万娟娟范德朋
Owner 碧沃丰生物科技(广东)股份有限公司
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