Preparation of acellular amniotic membrane carrier combined autologous endometrial stem cells

An endometrial and decellularization technology, applied in the field of biomedicine, can solve problems such as affecting the curative effect, and achieve the effects of preventing and controlling re-adhesion, prolonging the action time, and increasing the concentration.

Pending Publication Date: 2020-07-03
谭小军 +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recent studies have confirmed that hormone replacement therapy after intrauterine injection of endometrial stem cells can increase the thickness of the endometrium, but it is difficult to localize the injury after local injection, which affects the curative effect

Method used

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  • Preparation of acellular amniotic membrane carrier combined autologous endometrial stem cells
  • Preparation of acellular amniotic membrane carrier combined autologous endometrial stem cells
  • Preparation of acellular amniotic membrane carrier combined autologous endometrial stem cells

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Embodiment 1

[0022] 1. A preparation method of decellularized amniotic membrane carrier compounded with autologous endometrial stem cells.

[0023] 1. Equipment, materials and reagents used: ultra-clean bench, CO 2 Incubator, inverted microscope, centrifuge, vacuum freeze dryer, constant temperature water bath, pipette gun, cell culture dish, centrifuge tube, pipette, DMEM / F12 medium, fetal bovine serum, penicillin, streptomycin, 0.25 % trypsin-EDTA, normal saline.

[0024] 2. Primary isolation, culture, and passage of autologous endometrial stem cells: scrape and collect about 0.1ml of endometrial tissue, and immediately wash it with PBS buffer containing 100 IU / ml penicillin and streptomycin in a sterile environment. Wash 5 times, remove blood stains, cut into pieces to a diameter of 2 cultured in an incubator. Change the medium after 24 hours, and change the medium every 2 days. Observe the cell morphology and cell growth with an inverted microscope every day, and take pictures. It ca...

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Abstract

The invention relates to the preparation of acellular amniotic membrane carrier combined autologous endometrial stem cells. The isolating culture of autologous endometrial stem cells, the preparationand preservation of acellular amniotic membrane carriers and the preparation of acellular amniotic membrane carrier combined autologous endometrial stem cells are specifically included. The disadvantages of the occurrence of mechanical separation of intrauterine adhesions of hysteroscopic surgery in patients with moderate to severe intrauterine adhesions, the placing of intrauterine devices or anti-adhesion materials after operations, the endometrial functional repair which cannot be realized by giving estrogen to promote endometrial growth after the operations and the easy occurrence of re-adhesion can be overcome. The acellular amniotic membrane carriers have good anti-adhesion abilities, biocompatibility, biodegradability and safety; and as the autologous endometrial stem cells are taken as the active ingredients which can treat severe endometrial injury, growth factors can be secreted to improve local microenvironment and immunoregulation, so that the repair of damaged endometriumcan be promoted, and endometrial thickness and local vascular density can be increased.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the preparation of decellularized amniotic membrane scaffold compounded with autologous endometrial stem cells. Background technique [0002] The endometrium is divided into a functional layer and a basal layer from the inside to the outside. Under the action of sex hormones, periodic proliferation and exfoliation occur. At the same time, under the action of estrogen, the epithelial cells at the end of the basal glands proliferate to form a new functional layer. Cover the uterine body cavity to repair the wound surface. Therefore, even if there is necrotic exfoliation of the endometrium in each menstrual cycle, the front and rear walls of the uterus are close to each other and no adhesions will be formed. In patients with severe intrauterine adhesions, the basal layer is damaged and the glands disappear, resulting in the formation of epithelial cells and new blood vessels ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073A61L31/00A61L31/16
CPCA61L31/005A61L31/16A61L2300/412A61L2430/22C12N5/0682C12N2509/00C12N2533/92
Inventor 谭小军卢永娟黄洁琼
Owner 谭小军
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