Molecular marker related with cashmere fineness trait and detection primer and application of molecular marker related with cashmere fineness trait

A technology for molecular labeling and detection of primers, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problem of undiscovered cashmere fineness

Active Publication Date: 2020-07-07
SHENYANG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Although studies have shown that the NFKBIA gene may regulate the growth

Method used

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  • Molecular marker related with cashmere fineness trait and detection primer and application of molecular marker related with cashmere fineness trait
  • Molecular marker related with cashmere fineness trait and detection primer and application of molecular marker related with cashmere fineness trait
  • Molecular marker related with cashmere fineness trait and detection primer and application of molecular marker related with cashmere fineness trait

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Embodiment 1

[0023] This embodiment provides a molecular marker related to cashmere fineness traits, which includes the G1355A site of the cashmere goat NFK BIA gene; the nucleotide sequence of the G1355A site is shown in the sequence table SEQ ID NO:1, Specifically: ACRCCACTCCACTTGGCTG; wherein, R in the nucleotide sequence of the G1355A site is A or G; the AG genotype at the G1355A site can be used for the breeding of fine cashmere goats.

[0024] In addition, this embodiment also provides a detection primer for detecting the above-mentioned molecular markers, specifically, the detection primers include five sets of primer pairs, specifically, the detection primers are used to detect the Molecular marker; the detection primers include at least one of NFKBIA-1 primer pair, NFKBIA-2 primer pair, NFKBIA-3 primer pair, NFKBIA-4 / 5 primer pair and NFKBIA-6 primer pair; the NFKBIA- 1 The nucleotide sequence of the primer pair is shown in the sequence table SEQ ID NO: 2~3; the nucleotide sequenc...

Embodiment 2

[0029] This embodiment provides a method for detecting the mutation site in the cashmere goat NFKBIA gene using the above-mentioned detection primers, comprising the following steps:

[0030] S1. Obtain the DNA of the cashmere goat to be detected to obtain a DNA template;

[0031] S2. Perform PCR amplification on the detection primer and the DNA template to obtain a PCR amplification product;

[0032] S3. Sequencing the PCR amplification product to obtain a sequenced gene sequence;

[0033] S4. Comparing the sequenced gene sequence with the cashmere goat NFKBIA standard gene sequence to obtain the mutation site in the cashmere goat NFKBIA gene to be detected.

[0034] Concretely, in actual application, the above-mentioned method can be used in the experiment of finding the marker genotype related to the fineness economic traits of Liaoning cashmere goat cashmere, and its experimental method can include the following steps:

[0035](1) At the cashmere goat breeding farm in Li...

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Abstract

The invention is suitable for the field of a biological technology, and provides a molecular marker related with cashmere fineness trait and a detection primer and application of the molecular markerrelated with cashmere fineness trait. The molecular marker comprises a G1355A site of a down-producing goat NFKBIA gene, wherein the nucleotide sequence of the G1355A site is shown as a sequence tableSEQID NO:1, and R in the nucleotide sequence of the G1355A site is A or G. The advantageous genes of the G1355A site for cashmere fineness, cashmere yield, cashmere length and net cashmere rate are AG genetype. Therefore, the AG genetype of the G1355A site can be used for breeding of fine-cashmere down-producing goats to improve the condition that the quality of the cashmere of the down-producinggoats of Liaoning province is poor.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular marker related to cashmere fineness properties, detection primers and applications thereof. Background technique [0002] There are many kinds of cashmere goats, but they can basically be divided into two categories: one is cashmere goats in Russia, which mainly produce cashmere, which is characterized by short and long wool, and less wool than cashmere; the other is mainly produced in my country. The representative cashmere goat has long wool and short wool, and more wool than cashmere. Cashmere goats are crucial to the daily life of human society and are famous for their slender cashmere. Since cashmere is a high-grade and expensive textile raw material, the diameter of cashmere becomes one of the important factors determining the value of the fiber. Cashmere is produced in the secondary hair follicles of cashmere goat skin. The fiber diameter is 13-18 μm. T...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 王泽英白文林岳畅豆兴堂郭素平郑圆媛惠太宇王延茹李高谦岳奎华郭素玲王占红魏春梅武师良白智贤孙家明张鑫江蔡伟东
Owner SHENYANG AGRI UNIV
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