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Molecular markers related to cashmere fineness traits and their detection primers and applications

A technology of molecular markers and detection primers, which is applied in biochemical equipment and methods, microbe determination/inspection, DNA/RNA fragments, etc., can solve the problem of undiscovered cashmere fineness, and achieve the effect of improving the poor quality of cashmere

Active Publication Date: 2022-07-08
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although studies have shown that the NFKBIA gene may regulate the growth of cashmere, the effect on the fineness of cashmere has not yet been discovered.

Method used

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  • Molecular markers related to cashmere fineness traits and their detection primers and applications
  • Molecular markers related to cashmere fineness traits and their detection primers and applications
  • Molecular markers related to cashmere fineness traits and their detection primers and applications

Examples

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Embodiment 1

[0023] This embodiment provides a molecular marker related to the fineness of cashmere, which includes the G1355A site of the cashmere goat NFKBIA gene; the nucleotide sequence of the molecular marker is shown in SEQ ID NO: 1 in the sequence table, specifically: : ACRCCACTCCACTTGGCTG; wherein, the G1355A site is the R position in the nucleotide sequence, and its base is A or G; the AG genotype of the G1355A site can be used for the selection of fine cashmere goats.

[0024] In addition, this embodiment also provides a detection primer for detecting the above-mentioned molecular marker, specifically, the detection primer includes five sets of primer pairs, specifically, the detection primer is used to detect the detection primer according to claim 1 or 2. Molecular marker; the detection primers include at least one of NFKBIA-1 primer pair, NFKBIA-2 primer pair, NFKBIA-3 primer pair, NFKBIA-4 / 5 primer pair and NFKBIA-6 primer pair; the NFKBIA-1 The nucleotide sequences of the pr...

Embodiment 2

[0029] This embodiment provides a method for detecting mutation sites in cashmere goat NFKBIA gene using the above-mentioned detection primers, comprising the following steps:

[0030] S1. Obtain the DNA of the cashmere goat to be detected, and obtain a DNA template;

[0031] S2, performing PCR amplification on the detection primer and the DNA template to obtain a PCR amplification product;

[0032] S3, the PCR amplification product is sequenced to obtain a sequenced gene sequence;

[0033] S4. Compare the sequenced gene sequence with the cashmere goat NFKBIA standard gene sequence to obtain the mutation site in the cashmere goat NFKBIA gene to be detected.

[0034] Specifically, in practical applications, the above method can be used in the experiment of finding marker genotypes related to the economic traits of Liaoning cashmere cashmere fineness, and the experimental method can include the following steps:

[0035](1) In the cashmere goat breeding farm in Liaoyang City, L...

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Abstract

The invention is applicable to the field of biotechnology, and provides a molecular marker related to the fineness of cashmere and its detection primer and application. The molecular marker includes the G1355A site of cashmere goat NFKBIA gene; the nucleotide sequence of the molecular marker is As shown in SEQ ID NO: 1 of the sequence listing; the G1355A site is the R position in the nucleotide sequence, and its base is A or G. The dominant genotypes of the G1355A locus in cashmere fineness, cashmere yield, cashmere length and net cashmere rate are all AG genotypes. Therefore, the AG genotype of the G1355A locus can be used for the breeding of fine cashmere goats to improve the problems of poor cashmere quality of the existing Liaoning cashmere goats.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular marker related to the fineness character of cashmere, a detection primer and application thereof. Background technique [0002] There are many kinds of cashmere goats, but they can be basically divided into two categories: one is the cashmere goat in Russia, which mainly produces cashmere, which is characterized by long short hair and less hair than cashmere; the other is my country's cashmere goat. It represents the cashmere goat with long hair and short hair. Cashmere goat is very important to the daily life of human society and is famous for its slender cashmere. Because cashmere is a high-grade and expensive textile raw material, the diameter of cashmere has become one of the important factors in determining the value of the fiber. Cashmere is produced in the secondary hair follicles of the cashmere goat skin, with a fiber diameter of 13-18 μm. The total o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 王泽英白文林岳畅豆兴堂郭素平郑圆媛惠太宇王延茹李高谦岳奎华郭素玲王占红魏春梅武师良白智贤孙家明张鑫江蔡伟东
Owner SHENYANG AGRI UNIV
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