Early diagnosis method based on detection of specific antigen and coding gene thereof of hydatid

Abstract

The invention discloses an early diagnosis method based on detection of a specific antigen of a hydatid and a coding gene of the specific antigen. The invention relates to the technical field of echinococcosis diagnosis. The method comprises the steps: through the specific reaction after the antibody and the antigen are combined, combining an entomogenous antibody enzyme-labeled conjugate with thediluted excrement; after reaction for a period of time, utilizing microplate reader, and detecting the light absorption value of the reaction solution; comparing the light absorption value with the light absorption value of the reaction solution which is normally operated; obtaining results, i.e., obtaining whether the testee has the specific antigen of the hydatid or not; starting from the serumof a subject, wherein the free DNA molecules extracted from serum are more stable, and the accuracy of diagnosis data is improved; sequencing free DNA molecules by using a DNA next-generation sequencing technology, expanding a DNA sequence by using a PCR technology, and comparing with a reference sample sequence of a patient suffering from echinococcosis by using a bioinformatics analysis technology, thereby obtaining a conclusion whether the subject suffers from echinococcosis or not.

Description

technical field [0001] The invention relates to the technical field of echinococcosis diagnosis, in particular to an early diagnosis method based on the detection of echinococcosis specific antigen and its coding gene. Background technique [0002] Echinococcosis or echinococciosis is a chronic parasitic disease caused by human infection with the larvae (echinococcus) of Echinococcus. The clinical manifestations of this disease vary depending on the location, size and complications of the hydatid cyst. For a long time, hydatid disease has been considered as a zoonotic parasitic disease, and it is called an animal-derived disease. Recent epidemiological surveys show that it is called an endemic parasitic disease; it has the characteristics of occupational damage in endemic areas and is listed as an occupational disease of certain groups of people; globally speaking, echinococcosis is unique to ethnic minorities or religious tribes A common and frequently-occurring disease. ...

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Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the present invention provides an early diagnosis method based on the detection of echinococcosis-specific antigen and its coding gene, which solves the early diagnosis method for echinococcosis, usually through etiological diagnosis (imaging method, Including X-ray, ultrasonography and CT), indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA), etc., the above diagnostic methods have their own advantages, but the common defect is that echinococcosis can be diagnosed in the early stage due to small lesions. , it is difficult to detect, and some symptoms of cancer patients are similar to echinococcosis, and the requirements for professional and technical personnel are high, and the problems of missed and false detections are prone to occur, which is not only time-consuming and laborious, but also has a certain risk of harm to patients The problem

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