A kind of centipede algae extract and its application in the preparation of marine fouling biological control agent

A biological control agent, marine fouling technology, applied in the direction of biocides, plant growth regulators, chemicals for biological control, etc., can solve problems such as obvious seasonal changes

Active Publication Date: 2021-05-04
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Centipede algae (Grateloupia filicina) belongs to Rhodophycophyta (Rhodophycophyta) Cryptonemiaceae (Cryptonemiaceae). The change is obvious. It is a warm temperate algae in the world. It is distributed from south to north in the coastal waters of my country. It is an important economic seaweed with development prospects. And whether it can be applied to the field of marine fouling biological control, etc., there is no report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Preparation of centipede algae extract:

[0023] (1) Cut the centipede algae into small sections and put them in a jar, extract 3 times with 2 times the volume of absolute ethanol, combine the resulting ethanol extracts, and distill under reduced pressure to remove ethanol to obtain the crude extract extract;

[0024] (2) Chromatographic column for crude extract extract (Xinweier ) separation, using eluent ethyl acetate / petroleum ether to carry out gradient elution at a volume ratio of 0 to 10:10 to 0, to obtain 20 components;

[0025] (3) contrast by thin-layer chromatography spot plate (developing agent CH 2 Cl 2 / MeOH=10 / 3, volume ratio, RF value 0.45), combined components 15-18 (ethyl acetate / petroleum ether obtained by volume ratio 40:60, 50:50, 60:40, 70:30 elution Components), and then use Sephadex LH-20 chromatographic column with MeOH / CH 2 Cl 2 The elution is carried out according to the volume ratio of 1:1, part of the pigment is removed, the eluate is c...

Embodiment 2

[0027] Experimental group: the centipede algae extract (prepared in Example 1) was dissolved in methanol solvent to prepare a solution with a concentration of 282.6 μg / mL. Add 1 mL of this solution to a petri dish with a diameter of 6 cm, and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the dose of centipede algae extract coated on the bottom of the petri dish is 10 μg / cm 2 . Add 13 mL of sea water.

[0028] Control group: Add 1mL of methanol solvent to make it evenly distributed on the bottom of the petri dish, and then add 13mL of seawater after the volatilization is complete.

[0029] Blank group: add 13mL seawater.

[0030] Four parallel samples were set up in each of the experimental group, the blank group and the control group. Add 30 netted barnacle Venus larvae to each sample. Place in a constant temperature incubator at a temperature of about 30°C and cultivate in a dark environment. Statistical analysis was perf...

Embodiment 3

[0035] Experimental group: the centipede algae extract (prepared in Example 1) was dissolved in methanol solvent to prepare a solution with a concentration of 282.6 μg / mL. Add 1 mL of the solution to a petri dish with a diameter of 6 cm and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the dose of centipede algae extract coated on the bottom of the petri dish is 10 μg / cm 2 . Add 13 mL of sea water.

[0036] Control group: add 1mL of methanol solvent, so that the solvent is evenly distributed on the bottom of the petri dish, and then add 13mL of seawater after the solvent is completely evaporated.

[0037] Blank group: add 13mL seawater.

[0038] Four parallel samples were set up in the experimental group, the blank group and the control group, and about 30 vegetal disc larvae were added to each sample. Cultivate in a dark environment in an incubator at a temperature of about 26°C. Statistical analysis was performed on the a...

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PUM

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Abstract

The invention discloses a centipede algae extract and its application in preparing marine fouling biological control agent. When the centipede algae extract of the present invention is coated on a solid surface at a low dose, it has the effect of obviously inhibiting the attachment of barnacles and mussel larvae, so it can be used to prepare marine fouling biological control agents. And the centipede algae extract is extracted from centipede algae, which is a naturally occurring organic compound, which will not pollute the water environment and cause its enrichment in organisms through the food chain. It is environmentally friendly and safe, and can effectively inhibit While marine organisms are attached, it does not contain heavy metal elements such as copper and tin. It has good social benefits from the perspective of environmental protection, and its sources are extensive, and the separation and preparation methods are simple. It is suitable for large-scale production and has great promotion potential. It has a good application prospect in pest control.

Description

technical field [0001] The invention relates to the technical field of natural products, in particular to a centipede algae extract and its application in the preparation of marine fouling biological control agents. Background technique [0002] Marine fouling organisms will have extremely adverse effects on various sea-related activities. Not only will the ship's navigation resistance increase, the speed will decrease, and the fuel consumption will increase. Loading effect, endangering aquaculture facilities and objects, reducing yield and affecting quality. Therefore, it is an extremely important task to effectively prevent the harm of marine fouling organisms, and it is of great significance in production practice. [0003] The most harmful and difficult to remove members of marine fouling organisms are mainly sessile cirripedes (barnacles) with calcareous shells and bivalve molluscs (mussels and oysters). In tropical and subtropical coastal waters, reticulated barnacle...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/04A01P9/00A01P7/00
CPCA01N65/04
Inventor 严涛曹文浩佘志刚陈岩谢恩义林明晴
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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