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Fungus culture method for high-yield biological polysaccharide

A medium and solid medium technology, applied in the biological field, can solve the problems of low production of β-glucan, mixed with cellulose impurities, etc.

Pending Publication Date: 2020-07-24
ZHEJIANG GLLION BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The premise of producing β-glucan by fermentation is to obtain a fermentation broth with high β-glucan content. The traditional fermentation method usually has a low yield of β-glucan, or uses cellulose or its derivatives in the medium, resulting in Cellulosic impurities that are difficult to separate are mixed in the final product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0172]1) Take some fresh potatoes, peel them and cut them into uniform small pieces; weigh 200g, add 500mL of pure water, and boil for 60min; use a mixer to crush at high speed until it becomes muddy, and continue to boil for 60min; use double-layer gauze to filter, take Add 20g of glucose and 15-20g of agar to the filtrate, use purified water to make up to 1000mL, then boil in a water-proof bath until the agar is completely dissolved; sterilize by autoclaving at 121°C for 15 minutes; after the culture medium is cooled to about 65°C, pour it on a plate to prepare Improved PDA plate medium, ready for use;

[0173] 2) Take out the cryopreservation tube containing the Schizophyllum strain (purchased from China General Microorganism Culture Collection and Management Center CGMCC, No. 5.120) from the ultra-low temperature refrigerator, and put it into an ultra-clean workbench; place the cryopreservation tube in the palm of your hand to thaw, Then take 200 μL and add dropwise on the...

Embodiment 2

[0184] 1) Take some fresh potatoes, peel them and cut them into uniform small pieces; weigh 200g, add 500mL of pure water, boil until the potato pieces can be smashed by a glass rod, filter with eight layers of gauze, take the filtrate, and add 20g of glucose 15-20g agar and 10g sieved 20-80 mesh coarse particle size corn flour, use pure water to make up to 1000mL, then boil in a water-proof bath until the agar is completely dissolved; sterilize by autoclaving at 121°C for 15min; wait for the medium to cool to After about 65°C, invert the plate to make an improved PDA plate medium;

[0185] 2) Take out the cryopreservation tube containing the Schizophyllum strain from the ultra-low temperature refrigerator and put it into the ultra-clean workbench; put the cryopreservation tube in the palm of your hand to thaw, and then take 200 μL dropwise and culture it on the improved PDA plate prepared in 1). After coating evenly on the base, seal it with a flat-plate sealing film; place i...

Embodiment 3

[0196] 1) Take some fresh potatoes, peel them and cut them into uniform small pieces; weigh 200g, add 500mL of pure water, boil until the potato pieces can be smashed by a glass rod, filter with eight layers of gauze, take the filtrate, and add 20g of glucose , 15-20g agar and 5g sieved 20-80 mesh coarse-grained soybean meal powder, use purified water to make up to 1000mL, then boil in a water-proof bath until the agar is completely dissolved; sterilize by autoclaving at 121°C for 15min; wait for the medium to cool to After about 65°C, invert the plate to make an improved PDA plate medium;

[0197] 2) Take out the cryopreservation tube containing the Schizophyllum strain from the ultra-low temperature refrigerator and put it into the ultra-clean workbench; put the cryopreservation tube in the palm of your hand to thaw, and then take 200 μL dropwise and culture it on the improved PDA plate prepared in 1). After coating evenly on the base, seal it with a flat-plate sealing film;...

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Abstract

The invention provides a fungus culture method for high-yield biological polysaccharide. Specifically, the method adopts an improved PDA solid medium to activate schizophyllum commune, and through theimproved formula and preparation method of the PDA medium, the growth state and activity of schizophyllum commune on an improved PDA plate are significantly changed, and therefore passage of the plate is facilitated, the passage time is shortened, and also the yield of beta-glucan by fermentation culture is significantly improved. The method provided by the invention has the advantages of simpleoperation, low cost, very high yield of beta-glucan, no cellulose impurities, simple later separation and purification, and high purity.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for cultivating fungi with high yields of biopolysaccharides. Background technique [0002] Polysaccharides refer to a class of natural polymer compounds composed of more than 10 monosaccharide molecules, which are widely distributed in animals, plants, microorganisms (bacteria and fungi) and algal lichens. As an important biologically active substance, polysaccharides have the functions of regulating immunity, anti-tumor, reducing glycolipids, and delaying aging. They have broad application prospects in the fields of medical care, food, and animal breeding. Beta-glucan is a natural polysaccharide. Among them, mushroom glucan with β-1,3-glucosidic bonds as the backbone has the functions of enhancing immunity, anti-tumor, antibacterial, anti-virus, etc. It also has functions in moisturizing, anti-wrinkle, anti-oxidation, anti-ultraviolet, and promoting wound heal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/08C12N1/14A61K8/73A61K31/716A61Q19/00A23L33/125C12R1/645
CPCC12P19/08C12N1/14A61K8/73A61K31/716A61Q19/00A23L33/125A23V2002/00A23V2250/5034
Inventor 郭宏亮林檬庄秀园王轩朱勤健叶榛
Owner ZHEJIANG GLLION BIOTECH CO LTD
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