Preparation method and application of sweet potato leaf natural polypeptide
A natural technology of sweet potato leaves, applied in the field of natural polypeptides of sweet potato leaves and its preparation, can solve the problems of complex natural activity retention of polypeptide extraction methods, and achieve the effects of enhancing the body's immunity, enhancing metabolism, and lowering cholesterol
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Embodiment 1
[0018] Embodiment 1 A kind of preparation method of sweet potato leaf natural active polypeptide extract, the steps are as follows:
[0019] (1) Take sweet potato leaves, cut into cubes, homogenate in an ice bath at 4°C, then add 0.05mol / L pH7.0 phosphate buffer solution twice the weight of pre-cooled sweet potato leaves to soak, and place in a refrigerator at 4°C for 12 hours Afterwards, refrigerate and centrifuge at 4°C, and the supernatant is the crude extract containing natural polypeptides from sweet potato leaves;
[0020] (2) Slowly add the eluent to the crude extract containing the sweet potato leaf polypeptide obtained in step (1) to be diluted to a solution containing 50 mg / mL of the polypeptide, and use Sephadex G-50 column, inner diameter 1cm , the column length is 60cm, the eluent is 0.05mol / L, phosphate buffer solution of pH7.0, containing 0.15mol / L sodium chloride solution, the flow rate is 2mL / min, the injection volume is 1mL, and the detection wavelength is 28...
Embodiment 2
[0022] Embodiment 2 A kind of preparation method of sweet potato leaf natural active polypeptide extract, the steps are as follows:
[0023] (1) Take sweet potato leaves, cut into cubes, homogenate in an ice bath at 4°C, then add 0.2mol / L pH6.0 phosphate buffer solution 3 times the weight of pre-cooled sweet potato leaves to soak, and place in a refrigerator at 2-8°C After 12 hours, refrigerate and centrifuge at 2-8°C, and the supernatant is the crude extract containing natural polypeptides from sweet potato leaves;
[0024] (2) Slowly add the eluent to the crude extract containing the sweet potato leaf polypeptide obtained in step (1) to be diluted to a solution containing 100 mg / mL of the polypeptide, and use Sephadex G-50 column, inner diameter 1cm , the column length is 60cm, the eluent is 0.05mol / L, phosphate buffer solution of pH7.0, containing 0.15mol / L sodium chloride solution, the flow rate is 1mL / min, the injection volume is 1mL, and the detection wavelength is 280nm...
Embodiment 3
[0026] Embodiment 3 A kind of preparation method of sweet potato leaf natural active polypeptide extract, the steps are as follows:
[0027] (1) Take sweet potato leaves, cut into cubes, homogenate in an ice bath at 2-8°C, then add 0.02 mol / L pH7.2 phosphate buffer solution 1 times the weight of pre-cooled sweet potato leaves to soak, place in 2- Refrigerate at 8°C for 12 hours, refrigerate and centrifuge at 2-8°C, and the supernatant is the crude extract containing natural polypeptides from sweet potato leaves;
[0028] (2) Slowly add the eluent to the crude extract containing the sweet potato leaf polypeptide obtained in step (1) to be diluted to a solution containing 30 mg / mL of the polypeptide, and use Sephadex G-50 column, inner diameter 1cm , the column length is 60cm, the eluent is 0.05mol / L, phosphate buffer solution of pH7.0, containing 0.15mol / L sodium chloride solution, the flow rate is 0.5mL / min, the injection volume is 1mL, and the detection wavelength is 280nm) f...
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