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Preparation method and application of sweet potato leaf natural polypeptide

A natural technology of sweet potato leaves, applied in the field of natural polypeptides of sweet potato leaves and its preparation, can solve the problems of complex natural activity retention of polypeptide extraction methods, and achieve the effects of enhancing the body's immunity, enhancing metabolism, and lowering cholesterol

Inactive Publication Date: 2020-07-28
北京中科卓明生物医学研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention provides a simple polypeptide preparation method that directly extracts natural polypeptides from biological tissues and can retain the natural activity of polypeptides, so as to at least solve the problems of complex techniques and natural activity retention of polypeptide extraction methods in the prior art

Method used

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  • Preparation method and application of sweet potato leaf natural polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 A kind of preparation method of sweet potato leaf natural active polypeptide extract, the steps are as follows:

[0019] (1) Take sweet potato leaves, cut into cubes, homogenate in an ice bath at 4°C, then add 0.05mol / L pH7.0 phosphate buffer solution twice the weight of pre-cooled sweet potato leaves to soak, and place in a refrigerator at 4°C for 12 hours Afterwards, refrigerate and centrifuge at 4°C, and the supernatant is the crude extract containing natural polypeptides from sweet potato leaves;

[0020] (2) Slowly add the eluent to the crude extract containing the sweet potato leaf polypeptide obtained in step (1) to be diluted to a solution containing 50 mg / mL of the polypeptide, and use Sephadex G-50 column, inner diameter 1cm , the column length is 60cm, the eluent is 0.05mol / L, phosphate buffer solution of pH7.0, containing 0.15mol / L sodium chloride solution, the flow rate is 2mL / min, the injection volume is 1mL, and the detection wavelength is 28...

Embodiment 2

[0022] Embodiment 2 A kind of preparation method of sweet potato leaf natural active polypeptide extract, the steps are as follows:

[0023] (1) Take sweet potato leaves, cut into cubes, homogenate in an ice bath at 4°C, then add 0.2mol / L pH6.0 phosphate buffer solution 3 times the weight of pre-cooled sweet potato leaves to soak, and place in a refrigerator at 2-8°C After 12 hours, refrigerate and centrifuge at 2-8°C, and the supernatant is the crude extract containing natural polypeptides from sweet potato leaves;

[0024] (2) Slowly add the eluent to the crude extract containing the sweet potato leaf polypeptide obtained in step (1) to be diluted to a solution containing 100 mg / mL of the polypeptide, and use Sephadex G-50 column, inner diameter 1cm , the column length is 60cm, the eluent is 0.05mol / L, phosphate buffer solution of pH7.0, containing 0.15mol / L sodium chloride solution, the flow rate is 1mL / min, the injection volume is 1mL, and the detection wavelength is 280nm...

Embodiment 3

[0026] Embodiment 3 A kind of preparation method of sweet potato leaf natural active polypeptide extract, the steps are as follows:

[0027] (1) Take sweet potato leaves, cut into cubes, homogenate in an ice bath at 2-8°C, then add 0.02 mol / L pH7.2 phosphate buffer solution 1 times the weight of pre-cooled sweet potato leaves to soak, place in 2- Refrigerate at 8°C for 12 hours, refrigerate and centrifuge at 2-8°C, and the supernatant is the crude extract containing natural polypeptides from sweet potato leaves;

[0028] (2) Slowly add the eluent to the crude extract containing the sweet potato leaf polypeptide obtained in step (1) to be diluted to a solution containing 30 mg / mL of the polypeptide, and use Sephadex G-50 column, inner diameter 1cm , the column length is 60cm, the eluent is 0.05mol / L, phosphate buffer solution of pH7.0, containing 0.15mol / L sodium chloride solution, the flow rate is 0.5mL / min, the injection volume is 1mL, and the detection wavelength is 280nm) f...

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Abstract

The present invention provides a preparation method of sweet potato leaf natural polypeptide. The method comprises the following steps of washing and dicing sweet potato leaves, performing homogenizing at a low temperature, performing standing for 12 h at a low temperature, carrying out gel chromatography separation and purification on a supernatant extracting solution after centrifugation, collecting a part with the molecular weight of 500-12,000 Daltons, namely the sweet potato leaf natural polypeptide, and carrying out vacuum freeze drying on natural polypeptide collection liquid to obtainsweet potato leaf natural polypeptide powder. The sweet potato leaf natural polypeptide prepared by the preparation method provided by the invention is relatively low in molecular weight, retains relatively high natural activity and can be used for preparing health-care foods and foods.

Description

technical field [0001] The invention belongs to the field of natural product extraction, and in particular relates to a sweet potato leaf natural polypeptide and its preparation method and application. Background technique [0002] Sweet potato, also known as sweet potato, sweet potato, sweet potato, sweet potato, sweet potato, thread sweet potato, white potato, golden potato, sweet potato, red potato, pillow potato, etc., is an annual vine herb. Sweet potatoes have the effects of "replenishing deficiency, benefiting energy, invigorating the spleen and stomach, and strengthening kidney yin", making people "longevity and less disease". Sweet potato leaves are also a highly nutritious vegetable in daily life. Tests have shown that fresh sweet potato stems and leaves contain a large number of functional components: flavonoids, dietary fiber, active polysaccharides, carotenoids, protein complexes, etc. According to the latest research, sweet potato stems and leaves have medical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/16A23L33/18
CPCA23L33/18A23V2002/00C07K1/145C07K1/16A23V2250/55A23V2200/30A23V2200/302A23V2200/324A23V2200/326A23V2200/3262
Inventor 菅晓勇
Owner 北京中科卓明生物医学研究所有限公司
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