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Stem cell separation device

A separation device and stem cell technology, applied in the field of stem cell separation, can solve the problems of sample contamination, cell sieves affecting separation efficiency, etc., and achieve the effect of reducing clogging

Active Publication Date: 2020-08-07
广东康盾高新技术产业集团股份公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The stem cell separation device can use a cell screen for separation. Pressurizing the sample during the separation process can improve the separation efficiency. In the prior art, the air bag is used to pressurize to improve the separation efficiency, but the air bag pressurization will introduce external gas, even if Setting up a sterilizing filter for bacterial separation still has the risk of introducing bacteria to cause sample contamination. At the same time, the gas pressurization cannot form a reverse liquid flow to promote cell dispersion, and the blockage of the cell screen will affect the separation efficiency.

Method used

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Embodiment Construction

[0019] In order to better understand the technical content of the present invention, specific embodiments are provided below, and the present invention is further described in conjunction with the accompanying drawings.

[0020] see Figure 1~2 , a stem cell separation device, comprising a tank body 1, a sleeve 2, a cell mesh 3 and a water pushing piston 4, the sleeve 2 is provided with openings at both ends and is fixed in the tank body 1 by a bracket 5, the The water pushing piston 4 moves at the lower part of the sleeve 2, the cell screen 3 is arranged on the upper part of the sleeve 2, and a first filter is arranged between the sleeve 2 and the tank body 1 6. The sleeve 2 below the cell screen 3 is provided with a second filter 7, and the second filter 7 is provided with a first through hole 8, and the first through hole 8 is provided with a downward The first one-way baffle 9 that is opened, the side wall of the sleeve 2 is provided with a second through hole 10, and the...

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Abstract

The invention discloses a stem cell separation device. The device comprises a tank body, a sleeve, a cell screen and a water pushing piston, openings are formed in the two ends of the sleeve, the sleeve is fixed in the tank body through a support, the water pushing piston moves at the lower part of the sleeve, the cell screen is arranged at the upper part of the sleeve, a first filter screen is arranged between the sleeve and the tank body, a second filter screen is arranged in the sleeve below the cell screen, a first through hole is formed in the second filter screen, the first through holeis provided with a first one-way baffle which is opened downwards. A second through hole is formed in the side wall of the sleeve, a second one-way baffle which is opened outwards is arranged in the second through hole, a metal block is arranged in the water pushing piston, a magnet is arranged outside the tank body, the magnet and the metal block attract each other, and the magnet drives the metal block to move up and down along the tank body. According to the stem cell separation device, the cell separation efficiency is improved and cleaning and disinfection are facilitated.

Description

technical field [0001] The invention relates to the technical field of stem cell separation, in particular to a stem cell separation device. Background technique [0002] Stem cells are of great significance in the medical field. With the research of stem cells, the separation technology of stem cells has attracted more and more attention. The stem cell separation device can use a cell screen for separation. Pressurizing the sample during the separation process can improve the separation efficiency. In the prior art, the air bag is used to pressurize to improve the separation efficiency, but the air bag pressurization will introduce external gas, even if Setting up a sterilizing filter for bacterial separation still has the risk of introducing bacteria to cause sample contamination. At the same time, gas pressurization cannot form a reverse liquid flow to promote cell dispersion, and the blockage of the cell screen will affect the separation efficiency. Contents of the inv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/12C12M1/00
CPCC12M23/02C12M33/14C12M37/00C12M47/04
Inventor 湛振键齐国光
Owner 广东康盾高新技术产业集团股份公司