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Tile light sheet selective plane illumination microscope, its use method and microscope system

A selective and microscopic technology, which is applied in the direction of microscope, optics, optical components, etc., can solve the problems of reducing spatial resolution, reducing optical sectioning ability, and reducing the confinement ability of excitation beam, so as to reduce the amount of source data, speed up imaging, The effect of increasing the resolution

Active Publication Date: 2022-06-03
锘海生物科学仪器(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the size of the light sheet increases, especially when imaging large-volume samples, the confinement ability of the excitation beam of this "non-diffracting" light sheet decreases rapidly, which significantly reduces the optical sectioning ability and significantly reduces the spatial resolution.

Method used

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  • Tile light sheet selective plane illumination microscope, its use method and microscope system
  • Tile light sheet selective plane illumination microscope, its use method and microscope system
  • Tile light sheet selective plane illumination microscope, its use method and microscope system

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Embodiment Construction

[0045] In some embodiments, multiple tiling approaches may be employed. For example, in the propagation direction of the excitation light

[0047] FIG. 1 (d) shows a flowchart of a method for using TLS-SPIM according to an embodiment of the present disclosure. As shown in Figure 1(d),

[0049] In some embodiments, the pupil may be the entrance pupil of the excitation objective. Accordingly, the optical tuning of the SLM can be

[0054] In some embodiments, the pupil segments 2051, 2052, 2062, 2061 may adopt radially extending structures, and each group

[0055] In some embodiments, the area and addition of each pupil subsection 2051, 2052, 2062, 2061 can be adjusted.

[0056] In some embodiments, as shown in Figures 2(a) and 2(b), each coaxial excitation beam array 203 (or 204)

[0057] The combined phase map 208 can be created by loading the SLM together to include the first pair and the second pair of coaxial excitation lights

[0058] In some embodiments, the superposition of...

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Abstract

The present disclosure relates to a tiled light sheet selective plane illumination microscope, its use method and a microscope system. The usage method uses a spatial light modulator (SLM) to load corresponding phase maps to each group of pupil divisions of the pupil to phase-modulate the excitation beam, thereby creating at least two coaxial excitation beam arrays. The at least two coaxial excitation beam arrays created can be scanned to generate discontinuous light sheets accordingly. Furthermore, the generated at least one discontinuous light sheet can be tiled in the propagation direction of the excitation light to obtain a tiled light sheet for selective planar illumination of the sample. Utilizing the usage method of TLS-SPIM according to the present disclosure, TLS-SPIM and systems including the same, imaging speed can be significantly accelerated, resolution can be improved, and the amount of source data can be reduced.

Description

Technical field [0001] The present disclosure relates to a precision optical instrument and its use method and system, and more specifically, to a tiled light sheet selective plane illumination microscope, its use method and a tiled light sheet selective plane illumination microscope system. Background technique [0002] The 3D imaging capabilities of selective plane illumination microscopy (SPIM) (also known as light sheet microscopy) depend on the intensity distribution of the excitation light sheet used for 3D imaging. The thickness, light confinement capability and size of the light sheet determine the axial resolution, optical sectioning capability and field of view (FOV) of SPIM respectively. However, due to the diffraction of light, as the length of the light sheet increases, the constraint of the excitation light will decrease, making it impossible to take into account the thickness, light confinement capability, and size of the light sheet. This makes it difficult t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/06
CPCG02B21/06G02B21/0032G02B21/0076G02B21/006G02B21/367G02B27/58G02B21/362G02B21/0036
Inventor 高亮
Owner 锘海生物科学仪器(上海)有限公司