Light source device and holographic observation apparatus

Abstract

Provided are a light source device and a holographic observation apparatus. The invention provides a small light source device which can easily perform wave combination and has high light utilizationefficiency. The light source device is connected to an optical fiber and emit light from the optical fiber, and the light source device includes: a plurality of laser light sources to respectively emit light at different wavelengths; a current source to supply a drive current with a superimposed alternating-current component to each laser light source; a light source control section to selectivelyswitch the laser light sources by controlling the current sources; a plurality of optical systems disposed in optical paths of the respective laser light sources to reflect the light from the respective laser light sources to an incident end of the optical fiber and to reflect return light reflected on the incident end to the respective laser light sources; and a return light adjustment section to adjust an amount of the return light to continuously spread a spectrum of the light emitted from the optical fiber.

Description

technical field [0001] The present invention relates to a light source device used in a device for observing a target object using holography, and a holographic observation device including the light source device. Background technique [0002] Conventionally, a holographic observation device has been practically provided as a device for observing iPS cells or other cells. In such a holographic observation device, a beam (coherent beam) with the same phase is divided into two beams, one beam is irradiated on the target object to pass through the target object or reflected by the target object, and the other beam is kept as it is. A holographic image (hologram) is obtained by causing the two light beams to interfere at the imaging plane. Furthermore, since the obtained hologram contains information such as the three-dimensional shape and optical composition of the target object, such information is extracted by performing various calculations thereon. [0003] When it is de...

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Problems solved by technology

[0003] When it is desired to use such a holographic observation device to observe while keeping the cells alive, the cells are observed by floating in the culture medium in the container or mounted on a glass plate for observation. There are containers, glass plates, etc. other than the cells of the object, so the phase shift of the coherent light beam occurs not only by the object to be observed, but also by the container, glass plate, etc.

Also, when a phase shift occurs due to a container or a glass plate, etc., the interference due to the phase shift due to the container, glass plate, etc. affects the interference image due to the phase shift due to the observation target object (cell) (In other words, deteriorating the image quality of the hologram of the object to be observed (cell))

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