A kind of method utilizing garlic callus to produce allicin
A callus and allicin technology, applied in the field of agricultural bioengineering, can solve the problems of not fully meeting the market demand, many impurities in raw materials, and low product purity, and achieve no pesticide residues, strong controllability and uniformity in the whole process strong effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0041] (1) Callus induction and subculture using garlic bulb slices as explants:
[0042] Pick garlic bulbs without lesions and peel off their skins, rinse them with tap water for 5 minutes, and then use 0.1% (w / w) HgCl in an ultra-clean workbench after ultraviolet sterilization. 2 Soak for 20 min, repeatedly wash with sterile distilled water for 2 min, then soak with 75% (w / w) ethanol for 10 min, then repeatedly wash with sterile distilled water for 2 min for later use; cut garlic bulbs into 0.5 cm thick garlic slices, inoculate into Callus induction medium: MS + 30g / L sucrose + 1.5mg / L 2,4-D + 0.5mg / L KT + 7.8g / L agar pH=5.8, until enough yellow or light yellow granular Carry out 3 subcultures after the callus for standby;
[0043] (2) Culture of garlic callus high-yielding allicin cell line:
[0044] After the garlic callus was subcultured for 3 times, the vigorously growing garlic callus was selected and transferred to the allicin production medium: 1 / 2 MS+30g / L sucrose+...
Embodiment 2
[0048] (1) Callus induction and subculture using garlic bulb slices as explants:
[0049] Pick garlic bulbs without lesions and peel off their skins, rinse them with tap water for 5 minutes, and then use 0.1% (w / w) HgCl in an ultra-clean workbench after ultraviolet sterilization. 2 Soak for 20 min, repeatedly wash with sterile distilled water for 2 min, then soak with 75% (w / w) ethanol for 10 min, then repeatedly wash with sterile distilled water for 2 min for later use; cut garlic bulbs into 0.5 cm thick garlic slices, inoculate into Callus induction medium: MS + 30g / L sucrose + 1.5mg / L 2,4-D + 0.5mg / L KT + 7.8g / L agar pH=5.8, until enough yellow or light yellow granular Carry out 3 subcultures after the callus for standby;
[0050] (2) Culture and metabolic regulation of high-yield allicin cell line from garlic callus:
[0051] After the garlic callus was subcultured for 3 times, the vigorously growing garlic callus was selected and transferred to the allicin production me...
Embodiment 3
[0056] Comparative Test
[0057] Utilize the method of the present invention, without adding inducer SO 2 (NH 2 ) 2 The 1 / 2 MS culture medium was used as a control group, and a concentration of 300mg / L inducer SO was added 2 (NH 2 ) 2 The 1 / 2 MS medium was the control group, and the calli were cultured for 5, 10, 20, and 30 days to detect the content of allicin. 2 (NH 2 ) 2 After regulation, the allicin content was up to 89.43 μg / g (Table 1).
[0058] Table 1
[0059]
[0060] Conclusion: Compared with the control group, adding a concentration of 300mg / L inducer SO 2 (NH 2 ) 2 The content of allicin in the post-regulation group increased significantly, the highest was 89.43 μg / g, and the production capacity of allicin was significantly enhanced.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com