Method for producing allicin by using allium sativum callus

A technology of callus and allicin, applied in the field of agricultural bioengineering, can solve the problems of many impurities in raw materials, can not fully meet market demand, low product purity, etc., and achieves strong controllability, no pesticide residue problem, and strong uniformity. Effect

Active Publication Date: 2020-08-18
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical synthesis has many problems such as raw material impurities and low product purity.
With the continuous deepening of the research on the biological activity of allicin, its application range is more and more extensive, but the traditional production of allicin is restricted by many factors, and only relying on garlic extraction or chemical synthesis will not be able to fully meet the market demand

Method used

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  • Method for producing allicin by using allium sativum callus
  • Method for producing allicin by using allium sativum callus
  • Method for producing allicin by using allium sativum callus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Callus induction and subculture using garlic bulb slices as explants:

[0042] Pick garlic bulbs without lesions and peel off their skins, rinse them with tap water for 5 minutes, and then use 0.1% (w / w) HgCl in an ultra-clean workbench after ultraviolet sterilization. 2 Soak for 20 min, repeatedly wash with sterile distilled water for 2 min, then soak with 75% (w / w) ethanol for 10 min, then repeatedly wash with sterile distilled water for 2 min for later use; cut garlic bulbs into 0.5 cm thick garlic slices, inoculate into Callus induction medium: MS + 30g sucrose + 1.5mg / L 2,4-D + 0.5mg / L KT + 7.8g / L agar pH=5.8, until a sufficient amount of yellow or light yellow granular callus is induced After the organization, subculture was carried out for 3 times for later use;

[0043] (2) Culture of garlic callus high-yielding allicin cell line:

[0044] After the garlic callus was subcultured for 3 times, the vigorously growing garlic callus was selected and transferred...

Embodiment 2

[0048] (1) Callus induction and subculture using garlic bulb slices as explants:

[0049] Pick garlic bulbs without lesions and peel off their skins, rinse them with tap water for 5 minutes, and then use 0.1% (w / w) HgCl in an ultra-clean workbench after ultraviolet sterilization. 2 Soak for 20 min, repeatedly wash with sterile distilled water for 2 min, then soak with 75% (w / w) ethanol for 10 min, then repeatedly wash with sterile distilled water for 2 min for later use; cut garlic bulbs into 0.5 cm thick garlic slices, inoculate into Callus induction medium: MS + 30g sucrose + 1.5mg / L 2,4-D + 0.5mg / L KT + 7.8g / L agar pH=5.8, until a sufficient amount of yellow or light yellow granular callus is induced After the organization, subculture was carried out for 3 times for later use;

[0050] (2) Culture and metabolic regulation of high-yield allicin cell line from garlic callus:

[0051] After the garlic callus was subcultured for 3 times, the vigorously growing garlic callus w...

Embodiment 3

[0056] Comparative Test

[0057] Utilize the method of the present invention, without adding inducer SO 2 (NH 2 ) 2 The 1 / 2 MS culture medium was used as a control group, and a concentration of 300mg / L inducer SO was added 2 (NH 2 ) 2 The 1 / 2 MS medium of the control group was used as the control group, and the content of allicin was detected after the calli were cultured for 5, 10, 20, and 30 days respectively. 2 (NH 2 ) 2 After regulation, the allicin content was up to 89.43 μg / g (Table 1).

[0058] Table 1

[0059]

[0060] Conclusion: Compared with the control group, adding a concentration of 300mg / L inducer SO 2 (NH 2 ) 2 The content of allicin in the post-regulation group increased significantly, the highest was 89.43 μg / g, and the production capacity of allicin was significantly enhanced.

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Abstract

The invention discloses a method for efficiently producing allicin by using allium sativum callus. Baodi allium sativum bulbs are mainly used as an explant induction callus, SO2(NH2)2 is used as an inducer to regulate the allium sativum callus to efficiently produce allicin, the concentration of SO2(NH2)2 is 300 mg/L, culture is conducted for 30 days, the content of the allicin is up to 89.43 [mu]g/g and the increase rate of allium is up to 126% through detection. A production culture medium of the allicin comprises 1/2 MS, 30 g of sucrose, 1.5 mg/L of 2,4-D and 7.8 g/L of agar, and the pH is5.8. The method provides a new method and basis for stable and efficient production of allicin.

Description

[0001] The invention was funded by the transformation and popularization project of scientific and technological achievements of Tianjin Agricultural Commission (201302030); the application development fund project of Tianjin Normal University (52XK1505, 043135202-XK1707). technical field [0002] The invention belongs to the technical field of agricultural bioengineering, and relates to a method for culturing garlic callus and producing allicin, more specifically, constructing a method for efficiently producing allicin by using garlic callus. Background technique [0003] garlic( Allium sativum L. ) is an annual herb of the Liliaceae Allium genus, which is used as both food and medicine. my country is the main producer of garlic. Garlic is not only edible but also has health care functions, can treat many diseases, and can play a role in reducing the risk of certain diseases. Studies have reported that a variety of bioactive functions of garlic are mainly related to the al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 王振英尚云涛范宝莉刘晓颖刘兰党光兰桂霞张双力
Owner TIANJIN NORMAL UNIVERSITY
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