Method for indoor quick recognition of wheat resistance to wheat root rot Umbilical Verticillium chlamydosporium black embryo disease

A technology of wheat root rot and Helmintheroides flatus, applied in the direction of microorganism-based methods, seed and rhizome treatment, botanical equipment and methods, etc., can solve the problems of laborious results, instability, time-consuming, etc., and improve efficiency and time Short, the effect of improving accuracy and reliability

Active Publication Date: 2020-08-18
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved in the present invention is: according to the deficiencies such as time-consuming, laborious and unstable results in the current wheat root rot B. There is a significant correlation between the percentage of seedling leaf blight lesions caused by B. sorokiniana and the black embryo rate as an entry point, providing a method for indoor identification of wheat root rot B. sorokiniana leaf blight at seedling stage The method for calculating the black embryo rate by the lesion percentage; that is, the present invention provides a method for quickly identifying wheat in the room to the resistance of wheat root rot B.sorokiniana black embryo disease

Method used

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  • Method for indoor quick recognition of wheat resistance to wheat root rot Umbilical Verticillium chlamydosporium black embryo disease
  • Method for indoor quick recognition of wheat resistance to wheat root rot Umbilical Verticillium chlamydosporium black embryo disease
  • Method for indoor quick recognition of wheat resistance to wheat root rot Umbilical Verticillium chlamydosporium black embryo disease

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Effect test

Embodiment 1

[0057] In the winter of 2016, using the method of the present invention for quickly identifying the resistance of wheat to B.sorokiniana black germ disease, 54 wheat lines harvested in Xingyang were identified for their resistance to black germ disease. The detailed steps are as follows:

[0058] a, surface sterilization of wheat seeds: select 54 healthy and plump wheat seeds of 54 wheat lines and soak them in 70% (volume ratio) alcohol for 2 minutes, then rinse with sterilized distilled water for 3 times, and shake gently for 5 seconds after adding distilled water each time Bell, so that the alcohol remaining on the surface of the seeds is fully dissolved in water to prevent alcohol from inhibiting the germination of wheat seeds;

[0059]b. Cultivation of wheat seedlings: put the wheat seeds after surface sterilization in step a in a petri dish with a diameter of 9 cm, and add 2 layers of sterilized filter paper and 4 mL of sterilized distilled water in advance in each petri d...

Embodiment 2

[0073] Using the method for quickly identifying the resistance of wheat to B.sorokiniana black germ disease in the laboratory of the present invention, 10 disease-resistant wheat lines were identified for 3 consecutive years, and compared with the field identification results. The detailed steps are as follows:

[0074] a, surface sterilization of wheat seeds: choose healthy, plump wheat seeds and immerse in 70% (volume ratio) alcohol for 2 minutes, then rinse 3 times with sterilized distilled water, shake gently for 5 seconds after adding distilled water each time, let The alcohol remaining on the surface of the seeds is fully soluble in water to prevent the alcohol from inhibiting the germination of wheat seeds;

[0075] b. Cultivation of wheat seedlings: put the wheat seeds after surface sterilization in step a in a petri dish with a diameter of 9 cm, and add 2 layers of sterilized filter paper and 4 mL of sterilized distilled water in advance in each petri dish, each Place...

Embodiment 3

[0089] Using the indoor rapid identification method of the present invention for the resistance of wheat to B.sorokiniana black embryo disease, in March 2018, 2 disease-resistant strains were identified from 10 F4 and F5 high-generation lines, and field hybridization was carried out in April, compared with field identification , do hybridization one year in advance to improve breeding efficiency, the detailed steps are as follows:

[0090] a. Surface sterilization of wheat seeds: select 10 F 4 with F 5The healthy and plump seeds of the high-generation wheat line were soaked in 70% (volume ratio) alcohol for 2 minutes, then rinsed with sterilized distilled water for 3 times, and shook gently for 5 seconds after adding distilled water each time, so that the alcohol remaining on the surface of the seeds was fully Soluble in water to prevent alcohol from inhibiting the germination of wheat seeds;

[0091] b. Cultivation of wheat seedlings: put the wheat seeds after surface steri...

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Abstract

The invention discloses a method for indoor quick recognition of resistance of wheat to wheat root rot Ustilago esculenta black embryo disease. The method comprises the following steps: firstly, performing surface sterilization on wheat seeds, and performing seedling culture on the sterilized wheat seeds; preparing a wheat root rot boehmeria sorokiniana spore suspension, so as to obtain a spore suspension; shaking up the spore suspension liquid, spraying the spore suspension liquid onto wheat leaves with one leaf and one core for inoculation culture, and recording the percentage of the scab area of the first leaf of the wheat seedling in the total leaf area on the tenth day of inoculation culture, namely the scab area percentage; and calculating the wheat black embryo rate according to theequation, and evaluating the resistance of the wheat to the wheat root rot Umbilical Verticillium dahliae black embryo disease by utilizing the black embryo rate. Compared with an existing field recognition method, the recognition method has the advantages that the recognition time is shortened, the recognition link is simplified, the recognition efficiency is greatly improved, and the accuracy and reliability of the recognition result are improved.

Description

[0001] 1. Technical field: [0002] The invention belongs to the technical field of crop disease detection, and in particular relates to a method for quickly identifying the resistance of wheat to black germ disease of wheat root rot Bipolaris sorokiniana indoors. [0003] 2. Background technology: [0004] Black germ disease is a common disease of wheat, which means that there are black, brown and other disease spots on the surface of wheat grains in different shapes and degrees. Wheat black germ disease not only affects the appearance quality of wheat grains and leads to the reduction of wheat purchase grade, but also affects the emergence rate and seedling growth of wheat. What is more serious is that some black germ disease pathogens can produce toxins and seriously affect human health. The severity of wheat black germ disease varies with factors such as variety, location, year and control measures, and the incidence rate generally varies between 0.1 and 70.0% (see: 1, Li Q...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18A01G31/00C12R1/645
CPCC12Q1/18A01G31/00G01N33/0098A01C1/00
Inventor 李巧云李海泳姜玉梅牛吉山李梦钰王丝雨徐凯歌
Owner HENAN AGRICULTURAL UNIVERSITY
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