A disposable paper-based digital microfluidic detection chip and its detection method

Abstract

The invention provides a disposable paper-based digital microfluidic BNP marker detection chip, the chip includes: a lower plate and an upper plate, and the lower plate includes a substrate and an electrode layer in sequence from bottom to top A four-layer structure of a dielectric layer and a hydrophobic layer, the upper plate includes a transparent base and a transparent electrode. The detection chip can simplify the requirements for personnel operation and automatic sample processing, and can realize the operation of a variety of micro-droplet samples. It also has the characteristics of low cost, fast, ready-to-use, easy recycling and transportation, and has short detection time. , low detection limit and high sensitivity. In addition, the present invention also provides a method for rapid and automatic detection of BNP-B brain natriuretic peptide using the detection chip.

Description

technical field [0001] The invention belongs to the field of medical detection, and in particular relates to a disposable paper-based digital microfluidic detection chip and a method for detecting myocardial marker brain natriuretic peptide (BNP) using the chip. Background technique [0002] BNP-B brain natriuretic peptide, mainly from the ventricle. Its content is related to the pressure of the ventricle, the condition of the hormone regulation system during the process of dyspnea. The increase of ventricular volume and pressure can lead to the increase of BNP in plasma, and the degree of increase is proportional to ventricular dilation and pressure overload, which can sensitively and specifically reflect the changes of left ventricular function. Clinical detection of BNP concentration can assist in the diagnosis of heart failure, judgment of the severity of the disease and prognosis of recovery. The critical value of the judgment is 100pg / mL, and the risk of heart failure...

AI Technical Summary

Problems solved by technology

[0007] (1). For detection kit products, it generally configures the reagents required for the detection process, and provides experimental related equipment, such as centrifuge tubes, pipette tips, etc., and distributes them in a form similar to first aid kits after packaging , which saves the process of the operator configuring the relevant reagents, but at the same time, the kit still requires the operator to have certain medical knowledge and biochemical operation skills, and the actual operation process is still completed by people, and it is difficult to avoid the influence of human factors

[0008] (2). For test paper products, paper has been widely used as the base material of the product. People have realized various detection schemes on paper through various printing processes, such as chemical color development, colloidal gold immunochromatography, etc. It greatly simplifies the operation process. People without relevant medical knowledge only need to operate according to the instructions when using a specific test strip product. However, the disadvantage is that the detection items on the test strip platform are relatively limited, and it is difficult to realize complex processes and high-precision detection methods. , such as magnetic bead ELISA detection methods that require multiple elutions and sample additions, etc.

[0009] (3). For continuous flow microfluidic chip products, the common chips on the market are mostly pneumatically driven, micropump driven or centrifugally driven biochemical detection discs, detection chips, etc., and their materials are mostly hard resins such as PMMA, which can realize Rapid and automatic detection of trace samples in complex processes, but due to the small size of the product microchannel, there must be dead volume and cleaning problems after sample injection, and the degree of cleaning after multiple uses cannot be evaluated accordingly, which interferes with the accuracy of the test results. It is difficult to reuse for a long time, which also brings problems of environmental protection and recycling

For repeated detection, there are also problems of cleaning and discarding. At the same time, its production is more complicated than continuous flow microfluidic chips, and the recycling cost is higher.

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