Methods of treating phelan mcdermid syndrome using farnesyl dibenzodiazepinones
A technology of farnesyl dibenzodiazepine and syndrome, which is applied in the field of using farnesyl dibenzodiazepine to treat Phelan McDermid syndrome, can solve the problems of no approval and few clinical studies, etc.
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Embodiment 1
[0114] mouse
[0115] Shank3b with exon 13-16 deletion originally obtained from the Jackson lab - / - Homozygous mice, and wild-type (WT) littermates were generated on the C57BL / 6J background and repeatedly backcrossed to the C57BL / 6J background over eight generations. The resulting Shank3 knockout (KO) mice were placed in a temperature- and humidity-controlled room in groups of the same genotype, and subjected to a 12-hour light-dark cycle (lights on from 7 am to 7 pm). Room temperature and humidity were recorded continuously in the storage room. Tested during the light phase. Food and water were available ad libitum. Shank3 knockout mice and their wild-type littermates were tested, N=10 mice per treatment group, in behavioral experiments at two months of age. Mice were housed in commercial plastic cages and experiments were performed in accordance with the requirements of the UK Animals (Scientific Procedures) Act 1986 (UK Animals (Scientific Procedures) Act). All exper...
Embodiment 2
[0145] AMO-01 was administered to a 23-year-old male with genetically confirmed Phelan McDermid syndrome.
[0146] The drug under study was prepared from a sterile bulk formulation containing 32.8 mg / mL (3.15% w / w) of active drug substance dissolved in sterile 0.9% saline solution to make etc. Infiltrated final dosing formulation. Each vial contained 70 mg of AMO-01 drug substance in 2.134 mL of formulation (plus 2% excess). This corresponds to a total drug content of 71.4 mg of active drug substance per 2.176 mL of formulation. AMO-01 is administered as a single dose by intravenous infusion over 6 hours for a total administered dose of 120 mg / m 2 .
[0147] Seizure frequency and visual evoked potentials (VEPs) were assessed along with clinical testing. VEPs provide a non-invasive technique that assesses the functional integrity of the brain's visual pathways from the retina to the visual cortex via optic nerve / optic radiation. VEP was recorded from the surface of the hea...
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