A liquid discrete microfluidic chip and its application method

A microfluidic chip and liquid technology, applied in the field of microfluidics, can solve problems such as cumbersome operation steps, difficulty in integration and automation, and influence on analysis and detection results

Active Publication Date: 2022-03-04
东莞市东阳光诊断产品有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the existing technology of filling liquid into microchambers / micropores, the usual technology has the following disadvantages: some liquid discrete methods have relatively cumbersome operation steps, and some liquid discrete methods require skilled operators to complete better liquid filling effects , which will make it difficult to integrate and automate this liquid discretization method, and will also affect the analysis and detection results

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  • A liquid discrete microfluidic chip and its application method
  • A liquid discrete microfluidic chip and its application method
  • A liquid discrete microfluidic chip and its application method

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Embodiment 1

[0068] In this embodiment, the microfluidic chip of the present invention is described in detail by taking the application in the field of digital PCR as an example. The microfluidic chip is a centrifugal microfluidic chip with four sets of discrete units symmetrically arranged (one of which is shown in the figure), and each discrete unit includes a distribution unit 300 , three reaction tanks 100 , and a liquid pumping unit 200 . The distribution unit 300 includes a first liquid tank 310 , a distribution channel 320 and a waste liquid tank 330 . Wherein, the distribution channel 320 is connected to three reaction tanks 100 , each of the three reaction tanks 100 is connected to the liquid pumping unit 200 through the capillary channel 120 . The reaction tank 100 has a micro-reaction well array 110 composed of 20,000 micro-reaction wells 111 . The shape of the micro-reaction pool 111 is circular, the draft angle is 6°, the volume capacity is 0.1 nanoliter, the aspect ratio is ...

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Abstract

A liquid discrete microfluidic chip and a method for using the same. The present invention provides a microfluidic chip, comprising: at least one reaction tank, a micro-reaction pool array is arranged in the reaction tank, and the micro-reaction pool array is used to fill the first liquid; at least one liquid pumping unit, and The reaction tank is connected to transmit the second liquid to the reaction tank and cover the first liquid; wherein, the first liquid and the second liquid are incompatible with each other. The microfluidic chip only needs two steps of sample addition (adding the first liquid and adding the second liquid), which can quickly and effectively realize the discretization of the liquid, avoiding too many manual operations in the process, and is simple and easy to use. Low personnel requirements. Moreover, the microfluidic chip has a simple structure, is easy to be integrated and automated, and is easy to realize industrial production.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a liquid discrete microfluidic chip and its use method. Background technique [0002] In digital analysis and detection, such as digital PCR, it is necessary to discretize the sample solution, so that the original sample solution is split into several independent reaction units. The discretization of the sample solution is an extremely critical one in digital analysis and detection. step. However, in the existing technologies for filling liquids dispersedly into microchambers / micropores, there are usually the following disadvantages: some liquid dispersion methods have relatively cumbersome operation steps, and some liquid dispersion methods require skilled operators to achieve better liquid filling effects , which will make it difficult to integrate and automate this liquid discretization method, and will also affect the analysis and detection results. [0003] Therefore...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00B01L7/00B01J19/00C12M1/00C12M1/34
CPCB01L3/5027B01L3/502753B01J19/0093B01L7/52B01L2200/10
Inventor 陈跃东周侗顾志鹏刘仁源
Owner 东莞市东阳光诊断产品有限公司
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