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Long afterglow immunochromatographic test strip and detection method for detecting new coronavirus

A technology of immunochromatography test strips and long afterglow, which is applied in the field of immunochromatography detection, can solve the problems of weak luminescence of immunochromatography strips, and achieve the effects of accurate testing, easy promotion, and improved efficiency

Active Publication Date: 2022-02-22
FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a long afterglow immunochromatographic test strip and a detection method for detecting the new coronavirus, which solves the technical problems that the existing immunological test strips have weak luminescence and require professional equipment

Method used

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  • Long afterglow immunochromatographic test strip and detection method for detecting new coronavirus
  • Long afterglow immunochromatographic test strip and detection method for detecting new coronavirus
  • Long afterglow immunochromatographic test strip and detection method for detecting new coronavirus

Examples

Experimental program
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Effect test

Embodiment 1

[0075] The long afterglow material is composed of light absorbing agent and cache agent luminescent agent respectively; the light absorbing agent is The composition (the molar ratio of three light-absorbing agents is 2:1:5); the light-emitting agent is Caching agent is PdPc mainly absorbs ultraviolet, blue and near-infrared light bands; PtTPBP mainly absorbs ultraviolet, blue and red light bands; PdOEP mainly absorbs ultraviolet and green light bands. The luminescent wavelength of the luminescent agent is 615nm, which just falls in the range of visible light. The buffer transfer efficiency is also high enough to provide visible strength.

[0076] The luminescence mechanism of the long afterglow material composed of light absorbing agent and buffering agent luminescent agent is: after the light absorbing agent absorbs the excitation light, the energy level transitions to the excited state, and the unstable excited state transfers energy to oxygen to generate singlet oxygen,...

Embodiment 2

[0079] On the basis of Example 1, such as figure 1 As shown, the detection area is successively provided with IgM line (sprayed with anti-human IgG), IgG line (sprayed with anti-human IgG) and C line (sprayed with sheep anti-chicken IgY). The lgG line can intercept the new coronavirus S protein and the new coronavirus N protein; the lgM line can also intercept the new coronavirus S protein and the new coronavirus N protein, and the sheep anti-chicken lgY on the C line can interact with the chicken lgY that climbed up from the binding pad due to capillary action Specific binding, used to confirm whether the new coronavirus S protein and / or new coronavirus N protein on the binding pad is inactivated.

[0080] During the quality inspection of test strips, the sample to be tested (human anti-new crown standard, that is, the new crown positive sample) is added dropwise to the sample area of ​​the finished test strip, and the leading edge of the sample climbs up. The lines (quality...

Embodiment 3

[0083] Different from Example 2, the detection area in this example is only provided with IgM line (sprayed with anti-human IgM) and C line (sprayed with sheep anti-chicken IgY).

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Abstract

The long afterglow immunochromatographic test strip includes a bottom plate and a sample area, a detection area and an adsorption area which are sequentially connected on the bottom plate; the sample area contains long afterglow nanospheres coupled with the first biomarker; the long afterglow nanospheres Including polymer microspheres and long-lasting luminescent materials embedded in the polymer microspheres; the first biomarker can specifically bind to the target of the new coronavirus contained in the test sample; A detection line and a quality control line are set; the detection line is sprayed with a second biomarker that can specifically bind to the target to be detected; the quality control line is sprayed with an activity verification substance; the activity verification substance is used to indicate the effectiveness of the biomarker . The long afterglow immunochromatographic test strip has the advantages of convenient detection, high sensitivity, accurate test, low cost and easy promotion.

Description

technical field [0001] The invention belongs to the technical field of immunochromatography detection, in particular to a long-lasting immunochromatography test strip and a detection method for detecting novel coronavirus. Background technique [0002] Patients who are still asymptomatic after infection with the new coronavirus also have the risk of infecting others. The most effective way is to conduct professional immune tests on suspected patients to confirm whether they are dominant or recessive. Among them, the immune detection includes the detection of nucleic acid segments of the new coronavirus, specific proteins of the new coronavirus, and antibodies produced by the new coronavirus to be detected. [0003] The most commonly used in immunoassay is the immunological test strip. The fluorescent immunochromatographic test strip technology is based on the specific recognition between antigens and antibodies, and uses fluorescent microspheres as signal labels to realize t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/58G01N33/569G01N33/558
CPCG01N33/587G01N33/56983G01N33/558G01N2333/165G01N2469/20G01N2469/10
Inventor 李富友陈锐徐明葛霄鹏
Owner FUDAN UNIV
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