A DNA-wrapped near-infrared zone two nanoparticle complex and its preparation method and application

A nanoparticle and near-infrared technology, applied in the field of biomedicine, can solve the problems of damage to the normal function of the body, increased liver toxicity, and excessive intake of nanoparticles, and achieve long blood half-life, high biocompatibility, and fast liver metabolism. Effect

Active Publication Date: 2021-08-06
SHANGHAI JIAO TONG UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a DNA-wrapped near-infrared zone II nanoparticle complex and its preparation method and application, so as to solve the problem that the nanoparticle in the prior art has a large amount of uptake in the liver and spleen, and it is difficult to reach the target site to play a role. Increased toxicity, problems that impair the normal function of the body

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A DNA-wrapped near-infrared zone two nanoparticle complex and its preparation method and application
  • A DNA-wrapped near-infrared zone two nanoparticle complex and its preparation method and application
  • A DNA-wrapped near-infrared zone two nanoparticle complex and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Synthesis of surface carboxyl-modified Ag 2 S quantum dots, comprising the following steps:

[0041] 0.1mmol of silver diethyldithiocarbamate and 10g of dodecanethiol were mixed in a flask and placed in a N 2 In the atmosphere, the temperature was raised to 200 degrees Celsius and kept for 1 hour. Finally, after the solution was naturally cooled to room temperature, 50ml of absolute ethanol was added, centrifuged, washed, dispersed in cyclohexane, and then 0.15g of lipoic acid was added to the above system; Add an equal volume of absolute ethanol, stir for 48 hours, then centrifuge, wash with deionized water, and disperse with deionized water to obtain carboxyl-modified water-soluble Ag with a particle size of about 5 nm. 2 S quantum dots.

Embodiment 2

[0043] DNA-coated Ag 2 The preparation of S quantum dots comprises the following steps:

[0044] All DNA was dissolved in Q water and quantified to 100 μM by UV spectrophotometer. Synthesis of DNA double strands: short DNA-L and short DNA-R were mixed in PBS buffer with a final concentration of 50 μM, and reacted at 37°C for 30 minutes. Synthesis of DNA tetrahedron: TH32-1, TH32-2, TH32-3 and TH32-4 were mixed in PBS buffer at a final concentration of 1 μM, reacted at 95°C for 10min, and then rapidly cooled to 4°C.

[0045] 1) Weigh EDC (5.34mg) and NHS (18.45mg), dissolve EDC and NHS in the same buffer as the above DNA, and prepare a 2mmol / L solution. 2) Take 15 μL Ag 2 S-COOH (10μmol / L) quantum dots, add 50 times the amount of DNA (short DNA-L or long DNA) or 50 times the amount of DNA double strands (short DNA-L and short DNA-R) or The DNA tetrahedron of the amount of 4 times substance, then add 200 times excessive EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydr...

Embodiment 3

[0048] DNA-coated Ag 2 The application of S quantum dots in vivo imaging comprises the following steps:

[0049] The mice were anesthetized by intraperitoneal injection of chloral hydrate solution, and Ag 2 S quantum dots, Ag2S quantum dots wrapped in short-chain DNA, Ag2S quantum dots wrapped in long-chain DNA, Ag2S quantum dots wrapped in double-stranded DNA, Ag2S quantum dots wrapped in tetrahedron 2 S quantum dots were injected into nude mice through the tail vein at an input amount of 5 mg / kg, and placed in a near-infrared second-zone in vivo imager for observation at fixed time points (0.5h, 1h, 3h, 6h, 12h, 24h) to track Ag2S Distribution of quantum dots in the body, imaging conditions: excitation wavelength 880nm, laser intensity 3mW cm -2 , exposure time 50ms.

[0050] Result: The results show that ( Figure 5 ), adding the Ag that wraps the DNA 2 S quantum dots can significantly increase the metabolic rate of the liver, and the higher the DNA coverage, the faste...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention provides a DNA-wrapped near-infrared zone II nanoparticle complex and its preparation method and application. The preparation method comprises the following steps: S1: combining a solution containing functionalized DNA with a surface functional group Mixing the near-infrared region II nanoparticles, so that the functionalized DNA is covalently coupled to the functional groups on the surface of the near-infrared region II nanoparticles, to obtain a DNA-wrapped near-infrared region II nanoparticle complex; and S2: Inject the DNA-wrapped nanoparticle complex in the second near-infrared region into the experimental animal body through the tail vein, and observe the metabolism of the compound in the living body in the second-infrared near-infrared imaging system. The nanoparticle complex in the second near-infrared region prepared according to the present invention has the advantages of fast liver metabolism, long blood half-life, no obvious toxicity in cells and animals, high biocompatibility, etc., and has advantages in in vivo imaging and drug distribution. Significant advantage.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a DNA-wrapped near-infrared second region nanoparticle complex and a preparation method and application thereof. Background technique [0002] The current development of the biomedical field has changed from the traditional symptom-based treatment model to the information-based precision medical model, so the development of medical imaging technology will reflect and lead the progress of clinical medicine. There are many known imaging systems, such as carbon nanotubes, metal compounds, and nanoclusters, but there are very few imaging systems that are actually used in clinical practice. Limitations on the clinical application of imaging reagents mainly include two aspects. On the one hand, imaging reagents are difficult to quantify accurately in application, body tissues will produce light absorption and light diffusion, and fluorescent dyes will also have problems...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00
CPCA61K49/0019A61K49/0052
Inventor 樊春海李茜葛志磊赵彦
Owner SHANGHAI JIAO TONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap