Creation method and application of powdery mildew resistant novel wheat-rye 6BL-6RL small fragment translocation line

A 6BS.6BL-6RL, powdery mildew resistance technology, applied in the fields of molecular biology and cell biology, can solve problems such as linkage burden, genetic imbalance, etc., to increase the 1000-grain weight of seeds, enrich the genetic basis, and improve wheat yield traits. Effect

Pending Publication Date: 2020-09-18
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The powdery mildew resistance gene carried on the 6R chromosome of rye has broad-spectrum resistance, but due to linkage burden and genetic imbalance, the powdery mildew resistance gene carried on the 6R chromosome has not been successfully used in wheat so far. breed improvement

Method used

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  • Creation method and application of powdery mildew resistant novel wheat-rye 6BL-6RL small fragment translocation line
  • Creation method and application of powdery mildew resistant novel wheat-rye 6BL-6RL small fragment translocation line
  • Creation method and application of powdery mildew resistant novel wheat-rye 6BL-6RL small fragment translocation line

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Example 1 Creation of a novel wheat-rye 6BS.6BL-6RL small fragment translocation line resistant to powdery mildew

[0033] 1. Hybridization of wheat and rye to obtain octoploid triticale

[0034]The wheat cultivar Mianyang 11, which is highly susceptible to powdery mildew, was used as the female parent, and the diploid rye Kustro resistant to powdery mildew was used as the male parent. When crossing, the female parent Mianyang 11 needs to be emasculated in advance, and the selection of emasculation period and pollination period is the key factor for fruiting. The emasculation period is: the ear neck of the wheat ear exposes 1-2cm of the sword leaf sheath, and the pistil stigma is not fully opened. Pollination period: Pollination on the day of emasculation, and another pollination on the next day, so that a large number of F1 seeds can be obtained. After the obtained F1 seeds germinate and take root, cut off the root tip and use ND-FISH technology to identify whether i...

Embodiment 2

[0043] Example 2 Investigation of the Important Agronomic Traits of the Homozygous Wheat-Rye 6BS.6BL-6RL Small Fragment Translocation Line

[0044] The homozygous wheat rye 6BS.6BL-6RL small segment translocation line and its non-translocation sister line were planted under the same conditions, and their powdery mildew resistance and important agronomic traits, including plant height, tiller number, ear formation The number, spike length, number of spikelets per spike, seed setting rate, grain length and thousand-grain weight and other important agronomic traits were recorded, and the agronomic traits of homozygous translocation lines and non-translocation sister lines were statistically analyzed.

[0045] Such as figure 1 As shown, compared with the non-translocation sister line, the homozygous wheat rye 6BS.6BL-6RL small fragment translocation line was immune to powdery mildew, while the non-translocation sister line was highly susceptible to powdery mildew. Such as figur...

Embodiment 3

[0046] Example 3 Determination of the 6BL translocation breakpoint in the 6BS.6BL-6RL small fragment translocation chromosome, and the genes contained in the deletion 6BL and translocation 6RL segments

[0047] The homozygous 6BS.6BL-6RL small fragment translocation line was resequenced, and then the published wheat genome sequence (Wheat_IWGSC_WGA_v1.0) (http: / / www.wheatgenome.org) and rye genome sequence (Secale_cereale_Lo7_v2) ( https: / / wheat.pw.usda.gov / cgi-bin / seqserve / blast_rye.cgi) were combined, and then using bioinformatics methods, the resequencing results were compared with the combined genome sequences of wheat and rye to obtain Determine the 6BL break site in the 6BS.6BL-6RL small fragment translocation chromosome, and then use bioinformatics methods to analyze the genes carried by the missing 6BL segment and the 6RL segment involved in the translocation.

[0048] The oligonucleotide probes Oligo-pSc119.2-1, Oligo-Ku, Oligo-pSc200 and Oligo-pSc250 were used to det...

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Abstract

The invention belongs to the technical field of molecular biology and cytobiology, and particularly relates to a creation method and application of a powdery mildew resistant novel wheat-rye 6BS. 6BL-6RL small fragment translocation line. According to the creation method, the homozygous wheat-rye 6BS. 6BL-6RL small fragment translocation line is obtained by creating an octoploid triticale and powdery mildew resistant wheat-rye 6RL single-ended addition line through hybridization of wheat and rye and transferring a 6RL arm to different wheat backgrounds. The small fragment translocation line has no negative effects on important agronomic traits such as plant height, tillering capability, ear forming capability, ear length, ear number per ear, maturing rate, thousand seed weight and the likebesides powdery mildew resistance, and has an effect of increasing the thousand seed weight of seeds.

Description

technical field [0001] The invention belongs to the technical fields of molecular biology and cell biology, and in particular relates to a creation method and application of a novel wheat rye 6BS.6BL-6RL small fragment translocation line resistant to powdery mildew. Background technique [0002] Wheat powdery mildew is a worldwide disease, which is distributed in all major wheat-producing countries. my country's Shandong coast, Sichuan, Guizhou, and Yunnan occur commonly, and the damage is also serious. In recent years, the disease has also become more and more serious in the Northeast, North China, and Northwest wheat regions. Studies have shown that wheat varieties with high susceptibility to powdery mildew can cause 11.4-19.9% ​​yield reduction, and wheat varieties with moderate susceptibility to powdery mildew can cause 7.6-10.5% yield reduction. When wheat powdery mildew is severe, it may even cause a 34% reduction in production. In addition to reducing wheat yields,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04C12Q1/6895C12Q1/6841
CPCA01H1/02A01H1/04C12Q1/6895C12Q1/6841C12Q2600/156C12Q2600/13
Inventor 符书兰唐宗祥罗杰朱文倩汤述尧杜海梅邹杨杨杰智谭飞泉
Owner SICHUAN AGRI UNIV
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