Method for increasing wheat yield by creating wheat TaOTUB1 gene function deletion homozygous mutants

Abstract

The invention relates to a method for increasing wheat yield by creating wheat TaOTUB1 gene function deletion homozygous mutants. The method comprises the steps: searching proper sgRNA loci in wheat TaOTUB1 gene sequences, constructing wheat genome editing vectors PBUE411-sgRNA (otub1) and leading the wheat genome editing vectors PBUE411-sgRNA (otub1) into agrobacterium strains EHA105; performing genetic transformation on wheat immature embryo callus by the aid of the agrobacterium strains EHA105 carrying the PBUE411-sgRNA (otub1) vectors; screening three parts of wheat regeneration plants with homologous gene mutation of wheat TaOTUB1 by the aid of detection kits and enzyme digestion; performing generation addition and continuous selfing on the regeneration plants with TaOTUB1 gene mutation in greenhouses, screening TaOTUB1 gene function deletion homozygous mutant plants by the aid of HI-TOM detection kits and enzyme digestion, and identifying and screening plants without the PBUE411-sgRNA(otub1) editing vectors by smearing glufosinate-ammonium on leaves; and acquiring TaOTUB1 gene function deletion homozygous mutant plants otub1 without exogenous vectors. The tiller number and the grain number per spike are remarkably increased, the seed size and the grain weight are remarkably increased, the yield of grains of a single plant is remarkably increased, other characters are not remarkably affected, and the method has the advantages of orientation, rapidness, effectiveness and good repeatability.

Description

technical field [0001] The present invention relates to a method for improving wheat yield by using genome editing technology, and in particular to a method for improving wheat yield by creating a homozygous mutant of wheat TaOTUB1 gene function loss. The method can increase the number of effective tillers and the number of grains per plant of wheat, Increase thousand-grain weight and increase wheat grain yield. Background technique [0002] Wheat is one of the three staple crops in my country and even in the world, providing more than 20% of the calories and protein in the human diet. Increasing wheat yields is critical to global food and nutritional security (FAO, http: / / faostat.fao.org). Wheat yield is composed of the number of ears per mu, the number of grains per ear, and the thousand-grain weight; among them, the grain weight has high heritability and stability, and has great potential for improvement (Brinton and Uauy2019; Li, Xu et al.2019). Among the three compone...

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Problems solved by technology

According to the literature search conducted by the applicant, so far, there is no literature report that Ren Ji used genome editing technology to create a homozygous mutant of wheat TaOTUB1 gene function loss for improving wheat grain yield

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