A molecular marker for identifying the ms6 genotype of the nuclear male sterile line and its identification method

Abstract

The invention discloses a molecular marker for identifying the ms6 genotype of the nuclear male sterile line and an identification method thereof, the molecular marker for identifying the genotype of the nuclear male sterile line ms6 (including T295H and its derivatives), including specific primer sequences, The sequence of a pair of specific primers is: an upstream primer of SEQ ID NO.1 and a downstream primer of SEQ ID NO.2; the identification method includes the following steps: using SEQ ID NO.1 and SEQ ID NO.2 as primers to be Detect genomic DNA as a template for PCR amplification, digest the amplified product with MseI, and then separate and detect the PCR product and digested product by agarose gel electrophoresis; the wild type can be cut into 99bp, and the sterile line cannot It is cut to 129bp; heterozygotes will have two bands, and the small 99bp band will be weaker than the large 129bp band that cannot be cut. The test can be carried out at any developmental stage of soybean, and provides a simple, accurate and efficient new method for the molecular identification of the nuclear male sterile line ms6.

Description

technical field [0001] The invention belongs to the technical field of genotype molecular markers, in particular to a molecular marker for identifying the ms6 genotype of a nuclear male sterile line and an identification method thereof. Background technique [0002] Soybean is an important food crop in my country and provides rich protein for human beings. However, due to the low per unit yield and small area of ​​soybean planting, the total output is low, and a large amount of soybeans is dependent on imports. Therefore, it is an urgent problem to be solved in the current breeding work to breed high-yield soybean varieties and increase the yield of soybean planting. The method of recurrent selection in variety breeding can carry out group improvement. Since soybean is a self-pollinated crop, the flower organs are small, and artificial castration and pollination are relatively difficult, so it is relatively difficult to construct recurrent populations using conventional va...

AI Technical Summary

Problems solved by technology

Although there is a certain linkage relationship between the ms6 sterility locus and the white flower traits, there will still be a certain degree of non-linkage caused by crossover. Therefore, the ms6 sterility locus can be identified simply by the color of the stem or flower at the seedling stage. Not accurate enough, for this reason, we propose a kind of molecular marker and its identification method for identifying the ms6 genotype of the nuclear male sterile line to solve the problems mentioned in the above-mentioned background technology

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