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Geminivirus resistant plants

A technology of geminiviruses and plants, applied in the field of plant breeding and molecular biology, can solve the problems of virus transmission and growth

Pending Publication Date: 2020-09-25
主基因有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The spread of the virus is expected to grow further due to the overall movement of plant material and infestation of whitefly vectors

Method used

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  • Geminivirus resistant plants
  • Geminivirus resistant plants
  • Geminivirus resistant plants

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Embodiment Construction

[0061] The present invention relates to plant cells, plant tissues or plants having reduced expression of a wild-type protein, wherein the wild-type protein has at least about 75% sequence identity to SEQ ID NO: 1 over its entire length. The inventors have discovered that such a plant cell, tissue or plant has increased resistance to Geminiviridae compared to an otherwise identical plant cell, plant tissue or plant expressing a wild-type protein. More particularly, the inventors have discovered that modifying the gene encoding this wild-type protein, resulting in a nucleic acid sequence encoding a modified protein as further detailed herein, provides resistance against both dimorphic and unicolitic members of the family Geminiviridae . Additionally, the inventors have found that expression of a modified protein as defined herein maintains the vigor of the plants. Thus, the present invention represents an important advance in crop protection.

[0062] Thus, in a first aspect,...

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Abstract

The current invention pertains to a dicot plant cell, plant tissue or plant having a reduced expression of a wild type protein having at least 75% sequence identity with SEQ ID NO. 1. Furthermore, theinvention pertains to a plant cell, plant tissue or plant comprising a modified protein and / or a truncated protein having at least about 75% sequence identity when calculated over the positions corresponding to positions 1-66 and positions 78-308 of SEQ ID NO: 1, and wherein the modified protein increases the resistance to Geminiviridae as compared to an otherwise identical plant cell, plant tissue or plant expressing the protein without the modification or without the truncation. The invention further pertains to nucleic acid constructs encoding the modified or truncated protein. In addition, the invention relates to methods for generating a plant cell, plant tissue or plant comprising the modified protein that increases the resistance to Geminiviridae. Furthermore, the invention pertains to a plant cell, plant tissue or plant obtainable by the method of the invention.

Description

technical field [0001] The present invention relates to the fields of plant breeding and molecular biology. The present invention relates to plants carrying mutations conferring resistance to Geminiviridae. Background technique [0002] Insect-borne viruses cause some of the most destructive and economically important diseases to crop plants. Geminiviruses, a class of single-stranded DNA viruses with unique icosahedral virus particles, are responsible for many of these diseases. [0003] Geminiviruses comprise a biologically and genetically diverse family of viruses (Geminiviridae) characterized by biquasi-icosahedral virus particles (virions) measuring ~18 x 30 nm and ~2.9-5.2 A small circular single-stranded DNA genome of kilobases (kb). Based on genome structure, phylogenetic relationships, insect vectors, and host ranges, seven genera have been identified: Begomovirus, Mastrevirus, Curtovirus, Tomato pseudovirus Topocuvirus, Iranian Becurtovirus, Turncurtovirus and E...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/82
CPCC07K14/415C12N15/8283A01H1/06C12N15/8205
Inventor M·W·普林斯L·J·G·范恩科沃尔特H·P·韦尔斯勒伊斯
Owner 主基因有限公司