Method for detecting morchella mycelium nucleus concentration through nucleus staining

A technology of hickory chick and cell nucleus, which is applied in the field of nuclei staining and detection of hickory chick mycelial nuclei concentration, and can solve the problem of no morel mycelial cell apoptosis

Inactive Publication Date: 2020-09-29
CHANGSHU INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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However, in the Chinese and foreign patents and non-patent literatures disclosed so far, there is no technical information related...

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  • Method for detecting morchella mycelium nucleus concentration through nucleus staining
  • Method for detecting morchella mycelium nucleus concentration through nucleus staining
  • Method for detecting morchella mycelium nucleus concentration through nucleus staining

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Embodiment 1

[0030] The method for nuclear staining provided by the invention to detect hickory chick hyphae nuclei enrichment comprises the following steps:

[0031] A) hickory chick mycelia culture, the hickory chick described in the present embodiment is Liumei hickory chick, earlier uses puncher to be covered with Liumei hickory chick mycelium and is positioned at the PDA medium i.e. potato in the dish The edge place of glucose agar medium is perforated, specifically: around the edge position of PDA medium, it is preferred to punch several holes with a diameter of 6mm in an equidistant interval state, then inoculate respectively to the center of the PDA medium plate, and then in the plate Insert four coverslips with the same distance from the center of the petri dish obliquely on the PDA medium inside, and incubate at a constant temperature of 18°C ​​for 100 hours, so that the mycelia of Morchella chinensis will be covered with the coverslips;

[0032] B) for the treatment of the mycel...

Embodiment 2

[0038] The method for nuclear staining provided by the invention to detect hickory chick hyphae nuclei enrichment comprises the following steps:

[0039] A) hickory chick mycelia culture, the hickory chick described in the present embodiment is Liumei hickory chick, earlier uses puncher to be covered with Liumei hickory chick mycelium and is positioned at the PDA medium i.e. potato in the dish Perforate at the edge place of glucose agar medium, specifically: around the edge position of PDA medium preferably punch several holes that are 5mm in diameter with equidistant intervals, then inoculate respectively to the center of PDA medium plate, then in described plate Insert four coverslips with the same distance from the center of the petri dish obliquely on the PDA medium inside, and culture at a constant temperature of 20°C for 80 hours, so that the hyphae of Morchella spp. are covered with the coverslips;

[0040] B) For the treatment of the mycelia of Morchella chinensis, fir...

Embodiment 3

[0045] The method for nuclear staining provided by the invention to detect hickory chick hyphae nuclei enrichment comprises the following steps:

[0046] A) hickory chick mycelia culture, the hickory chick described in the present embodiment is Liumei hickory chick, earlier uses puncher to be covered with Liumei hickory chick mycelium and is positioned at the PDA medium i.e. potato in the dish The edge place of glucose agar medium is perforated, specifically: around the edge position of PDA medium, it is preferred to punch several holes with a diameter of 4mm in an equidistant interval state, then inoculate to the PDA medium plate center respectively, then in the four On the PDA medium in each plate, insert four coverslips at an equal distance from the center of the plate, and culture at a constant temperature of 22° C. for 72 hours, so that the hyphae of Morchella spp. are covered with the coverslips;

[0047] B) For the treatment of the mycelia of Morchella chinensis, first ...

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Abstract

The invention relates to a method for detecting morchella mycelium nucleus concentration through nucleus staining. The method comprises the following steps: carrying out mycelium culture by using a sterile plate containing a PDA culture medium; taking out cover glass covered with morchella mycelium from a culture medium, and dividing the cover glass into a control group and an experimental group;soaking the cover glass of the control group and the experimental group in a solvent, washing the cover glass after soaking is finished, and dropwise adding a fixing solution to the cover glass afterwashing is finished; washing the morchella mycelia fixed by the fixing solution by using a buffer solution, putting the cover glass of the control group and the experimental group into a clean dyeingbox, and adding a dyeing fixing solution into the dyeing box; washing the dyed morchella mycelia by using a buffer solution, sucking the residual buffer solution on the cover glass by using filter paper, and dropwise adding an anti-fluorescence quenching slide sealing solution into the center of the cover glass, so that the morchella mycelia are in contact with the anti-fluorescence quenching slide sealing solution to prepare slide samples of the control group and the experimental group. Understanding of factors such as growth, development, propagation and senescence of morchella esculenta andgrowth vigor and yield in the cultivation process is facilitated, and prejudgment is facilitated.

Description

technical field [0001] The invention belongs to the technical field of edible fungus hyphae cell detection, and in particular relates to a method for detecting morel mycelium nucleus concentration by cell nucleus staining. Background technique [0002] The aforementioned morels ( Morchella spp. ) Also known as delicious morel, commonly known as goat sparrow, corn fungus and so on. Because the surface of the mushroom cap is uneven and shaped like morel, it is called morel, and morel is also included in the book "Compendium of Materia Medica" by Li Shizhen. Morchella is a relatively precious natural tonic, rich in protein, multivitamins and more than 20 kinds of amino acids, delicious and nutritious. Morel mushrooms and Cordyceps sinensis have similar functions, and they are natural tonics that do not contain any hormones or side effects. In particular, modern medical research shows that Morchella has the functions of lowering blood fat, regulating immune function, anti-agi...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N1/30
CPCG01N1/30G01N21/6428G01N21/6486G01N2021/6432G01N2021/6439
Inventor 宋驰肖湘月张宇晨俞晓明冀宏蔡小龙陈业虎吴亮亮
Owner CHANGSHU INSTITUTE OF TECHNOLOGY
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