Composition for resisting aging

A composition and anti-aging technology, applied in the direction of drug combination, medical raw materials derived from mammals, anti-toxic agents, etc., can solve problems such as side effects

Active Publication Date: 2020-10-02
MERIDIGEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, although these natural substances have been researche

Method used

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  • Composition for resisting aging
  • Composition for resisting aging
  • Composition for resisting aging

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: The conditioned medium of umbilical cord mesenchymal stem cells restores the mitochondrial activity of damaged epithelial cells

[0026] 1. Materials and methods

[0027] 1.1 Preparation of umbilical cord mesenchymal stem cell conditioned medium

[0028] Seed HEK-293 cells (5x10 4 cells / well) for 24 hours. Inoculate umbilical cord mesenchymal stem cells (5x10 4 cells / well) for 24 hours. Remove the medium in the 24-well plate and add the medium containing 200 μM H 2 o 2 culture medium for 4 hours. Remove the medium in the transwell culture dish and add the basal medium. h 2 o 2 After 4 hours of treatment, the transwell culture plate was placed on a 24-well plate, and the umbilical cord mesenchymal stem cells and HEK-293 cells were co-cultured in a humidified incubator at 37°C for 44 hours. Afterwards, the medium in the transwell culture plate and the 24-well plate was collected, which was the umbilical cord mesenchymal stem cell conditioned medium, a...

Embodiment 2

[0033] Example 2: Conditioned Medium of Umbilical Cord Mesenchymal Stem Cells Reduces the Aging Degree of Damaged Epithelial Cells

[0034] 1. Materials and methods

[0035] 1.1 Preparation of umbilical cord mesenchymal stem cell conditioned medium

[0036] Prepared by the same method as in Example 1.

[0037] 1.2 Aging analysis of HEK-293 cells

[0038] Seed HEK-293 cells (4x10 4 cells / well) for 24 hours. Remove the medium in the 24-well plate and add the medium containing 200 μM H 2 o 2 culture medium for 4 hours. Thaw umbilical cord mesenchymal stem cell-conditioned medium at 4 °C. h 2 o 2 After 4 hours of treatment, add basal medium or umbilical cord mesenchymal stem cell conditioned medium as above, and co-culture HEK-293 cells in a humidified incubator at 37°C for 48 hours. After that, remove the medium and add the medium containing 100 nM bafilomycin A1, cultivate for 1 hour, remove the medium and add the medium containing 33 μM C 12 The culture medium of FDG...

Embodiment 3

[0041] Example 3: Conditioned Medium of Umbilical Cord Mesenchymal Stem Cells Reduces the Aging Degree of Damaged Skin Cells

[0042] 1. Materials and methods

[0043] 1.1 Preparation of umbilical cord mesenchymal stem cell conditioned medium

[0044] Prepared by the same method as in Example 1.

[0045] 1.2 Aging analysis of HaCaT cells

[0046] Seed HaCaT cells (2x10 5 cells / well) for 24 hours. Remove the medium and add 200 μM H 2 o 2 culture medium for 4 hours. Thaw umbilical cord mesenchymal stem cell-conditioned medium at 4 °C. h 2 o 2 After 4 hours of treatment, add basal medium or umbilical cord mesenchymal stem cell-conditioned medium as above, and co-culture with HaCaT cells in a humidified incubator at 37°C for 72 hours. Afterwards, remove the medium and add the medium containing 100 nM bafilomycin A1, cultivate for 1 hour, remove the medium and add the medium containing 33 μM 12 The culture medium of FDG was incubated for 2 hours. Then, remove the medium...

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Abstract

The invention discloses a composition for resisting aging. The composition comprises a conditioned medium, wherein the conditioned medium is obtained by co-culturing umbilical cord mesenchymal stem cells and cells damaged by oxidative pressure. The invention further discloses application of the conditioned medium. The conditioned medium is used for preparing the pharmaceutical composition for resisting aging, wherein the conditioned medium is obtained by co-culturing the umbilical cord mesenchymal stem cells and the cells damaged by oxidative pressure.

Description

technical field [0001] The present invention relates to a composition for antiaging. The present invention also relates to the use of a conditioned medium for preparing a pharmaceutical composition for anti-aging. Background technique [0002] Mitochondria are very important organelles in cells, and their functions are to perform respiration and synthesize ATP (adenosinetriphosphate), thereby regulating cellular energy metabolism[1,2]. When mitochondria are damaged, intracellular free radicals accumulate in large quantities, which in turn affects mitochondrial production of ATP and cellular energy metabolism, eventually leading to cell aging[3,4]. Therefore, many natural substances such as vitamin C, vitamin E, and β-carotene that have anti-oxidation or restore mitochondrial activity are widely used in food additives or skin care products. However, although these natural substances have researches to prove their efficacy, excessive use still has side effects. For example,...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61P39/00A61K8/99A61Q19/08A61K35/12
CPCA61K35/28A61K35/12A61P39/00A61K8/99A61Q19/08A61K2300/00
Inventor 宣昶有林卫理苏郁清施宏达杜茂宽吴汤博仲
Owner MERIDIGEN BIOTECH
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