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Immune cell treated by magnetic field and application thereof

A technology of immune cells and cells, applied in the field of immune cells, can solve problems such as lack of in vivo data support

Active Publication Date: 2020-10-02
HEYE HEALTH TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the molecular mechanism of how static magnetic fields affect T cell behavior is unclear and lacks support from in vivo data

Method used

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  • Immune cell treated by magnetic field and application thereof
  • Immune cell treated by magnetic field and application thereof
  • Immune cell treated by magnetic field and application thereof

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Experimental program
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Effect test

Embodiment

[0166] 1. Experimental materials and methods

[0167] 1. Mice

[0168] C57BL / 6 wild-type mice were purchased from SLAC, and OT-I TCR transgenic mice and PyMT mice were from the Jackson Laboratory in the United States. All experimental mice were raised in the SPF animal room of the Institute of Biochemistry and Cell Biology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences. All animal experiments were approved by the Animal Management Committee of the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences.

[0169] 2. Cell lines

[0170] B16-F10-OVA and EL-4 cells were provided by Xu Chenqi, a researcher from the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. B16-F10 cells were cultured with DMED medium: 10% serum, l-glutamine (2mM), penicillin (100U / ml), streptin (10U / ml). EL-4 cells were cultured with RPMI1640 medium: 10% serum, l-glutamine (2mM), penicillin (100U / ml), str...

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Abstract

The invention relates to an immune cell treated by a magnetic field and application thereof. According to the invention, a medium static magnetic field with the intensity of 0.3T can promote secretionof CD8<+> T cell granzyme and cell factors of a mouse, increase the level of ATP and mitochondrial respiration and up-regulate expression of related genes Uqcrb and / or Ndefs 6 of a mitochondrial respiratory chain. In addition, magnetic receptor candidate genes Isca1 and Cry1 / Cry2 participate in regulation and control of expression of Uqcrb and / or Ndefs 6. An in-vivo experiment shows that the static magnetic field can inhibit growth and development of tumors by promoting secretion of the CD8<+>T cell granzyme and the cell factors. A killing ability test shows that static magnetic field treatment can enhance the killing ability of CD8<+> T cells, and the CD8<+> T cells treated by the magnetic field have a remarkable anti-tumor effect when being transfused back into a tumor grafted mouse.The invention not only discloses that the medium-intensity static magnetic field can enhance the killing ability of the CD8<+> T cells by promoting mitochondrial respiration, but also provides a novel method for enhancing the anti-tumor function of the CD8<+> T cells by a physical method.

Description

Technical field [0001] The invention relates to the field of immune cells, in particular to immune cells treated with a magnetic field and their uses. Background technique [0002] T cells are an important part of adaptive immunity. T cells can be divided into CD4 based on the expression of CD4 and CD8 molecules on the surface + T cells and CD8 + T cells. CD4 + T cells, also known as helper T cells, mainly regulate and activate the activity of other immune cells by releasing secreted factors such as IFNγ, IL-4, IL-17 and TNFα. CD8 + T cells, also known as killer T cells (CTLs), can directly kill infected, damaged and non-functional cells, and play a key role in anti-tumor immunity. CTLs killing tumor target cells require perforin-mediated release of granzyme, especially granzyme B (granzyme B) is the main granule component. In addition, CTLs also secrete IFNγ and TNFα to destroy tumor cells. [0003] The magnetic field, like the temperature and pressure in the environment, is a...

Claims

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Application Information

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IPC IPC(8): C12N13/00C12N5/0781C12N5/0783C12N5/0784C12N5/0786C12N5/0787A61K35/17A61K35/15A61P35/00A61P35/02
CPCC12N13/00C12N5/0635C12N5/0636C12N5/0639C12N5/0645C12N5/0642A61K35/17A61K35/15A61P35/00A61P35/02C12N2529/00C12N2501/515C12N2501/51A61K39/39A61K39/46449A61K39/4611A61K2239/57A61K2300/00
Inventor 朱小燕方志财方彦雯刘岩曹献霞刘海峰
Owner HEYE HEALTH TECH CO LTD
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