Detection reagent for biomarker used for authenticating and judging metastatic and non-metastatic pancreatic cancer, and application

A technology of biomarkers and detection reagents, applied in the field of differential diagnosis of cancer, can solve the problem of not discovering and verifying biomarkers of pancreatic cancer, etc.

Inactive Publication Date: 2020-10-02
上海思路迪医学检验所有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, these researches on blood extracellular vesicle miRNA as a biomarker in the identification of pancreatic cancer metastasis are few, and no biomarkers that can be used to improve the specificity of pancreatic cancer metastasis have been found and verified.

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  • Detection reagent for biomarker used for authenticating and judging metastatic and non-metastatic pancreatic cancer, and application
  • Detection reagent for biomarker used for authenticating and judging metastatic and non-metastatic pancreatic cancer, and application
  • Detection reagent for biomarker used for authenticating and judging metastatic and non-metastatic pancreatic cancer, and application

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Embodiment Construction

[0028] In order to understand the content of the present invention more clearly, it will be described in detail with reference to the drawings and embodiments.

[0029] All miRNAs involved in the present invention are mature miRNAs annotated in the miRBase database (http: / / www.mirbase.org / ).

[0030] The blood extracellular vesicle miRNA biomarker of the present invention establishes a differential diagnosis model according to its expression value to assist in distinguishing patients with metastatic and non-metastatic pancreatic cancer, thereby improving the accuracy of diagnosis of metastatic pancreatic cancer.

[0031] The screening process of blood extracellular vesicle miRNA biomarkers hsa-miR-335-5p and hsa-miR-340-5p specifically detected by the detection reagent of the present invention comprises the following steps:

[0032] (1) Study cohort and clinical information

[0033] In this study, a total of 69 patients were collected and selected into two research cohorts, a...

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Abstract

The invention relates to a detection reagent for a biomarker used for authenticating and judging metastatic and non-metastatic pancreatic cancer, in particular to the biomarker which comprises blood extracellular vesicle miRNA: has-miR-335-5p and has-miR-340-5P and is used for distinguishing the metastatic and non-metastatic pancreatic cancer. The invention puts forward for the first time and verifies that when the blood extracellular vesicle miRNA is used as the biomarker and the expression level of the blood extracellular vesicle miRNA is used for distinguishing the metastatic and non-metastatic pancreatic cancer, sensitivity is high, and the detection reagent has an important meaning for authenticating and diagnosing the pancreatic cancer.

Description

technical field [0001] The invention relates to the technical field of cancer differential diagnosis, in particular to a detection reagent and application of a biomarker used for the differential diagnosis of metastatic and non-metastatic pancreatic cancer. Background technique [0002] Pancreatic cancer is a group of highly malignant and aggressive diseases of the digestive system, of which more than 90% are pancreatic ductal adenocarcinomas. The early onset of pancreatic cancer is asymptomatic. About 80% of pancreatic cancer patients are already in the middle and late stage when they are first diagnosed, and about 60% of the patients have distant metastasis of pancreatic cancer cells to the liver, lung and surrounding peritoneum, etc., making it difficult to perform surgery After resection, the five-year survival rate of patients is only 2%-9%. At present, surgical resection is still an important and effective method for the treatment of pancreatic cancer, but only 20% of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 尹磊张大东许晓雅年宝宁刘士毅王晓楠张亚楠陈灏郭世伟金钢
Owner 上海思路迪医学检验所有限公司
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