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Osteoclast differentiation inhibitor containing urolithin

A technology of differentiation inhibition and osteoclasts, applied in cosmetic preparations, active ingredients of heterocyclic compounds, bone diseases, etc., can solve the problems of urolithin hematopoietic stem cell differentiation that are not yet known, and achieve high safety effects

Pending Publication Date: 2020-10-02
DAICEL CHEM IND LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, it is not known that urolithins have the effect of inhibiting the differentiation of hematopoietic stem cells into osteoclasts

Method used

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  • Osteoclast differentiation inhibitor containing urolithin
  • Osteoclast differentiation inhibitor containing urolithin
  • Osteoclast differentiation inhibitor containing urolithin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0136] The isolated mouse bone marrow-derived hematopoietic stem cells were divided into 4.0×10 4 Cells / well were seeded in 96-well plates. Simultaneously with inoculation, 30 ng / mL of M-CSF (macrophage colony-stimulating factor, R&D Systems), 50 ng / mL of RANKL (receptor activator of nuclear factor kappa B ligand, PeproTech Inc.) were added to all wells as Inducer of differentiation into osteoclasts. In addition, urolithin A (1, 10, 25 μM) was added simultaneously with the differentiation inducer. At 37°C, 5% CO 2 After standing and culturing for 8 days in an incubator with a high concentration, remove the culture medium of each well, let stand in 10N Mildform for 10 minutes, fix the cells, and then wash 3 times with pure water. Then, 50 μL of TRAP staining solution (SIGMA) was added to each well, and incubated at 37° C. for 1 hour in a light-shielded state. The TRAP staining solution in each well was discarded, and after washing with pure water, 50 μL of Mayer's hematoxyl...

Embodiment 2

[0145] It was the same as Example 1 except that urolithin A was changed to urolithin B, and the added urolithin B was 25 or 50 μM.

Embodiment 3

[0154] Macrophages differentiated from hematopoietic stem cells isolated from mouse bone marrow were 4.0×10 4 Cells / well were seeded in 96-well plates. Simultaneously with inoculation, 30 ng / mL of M-CSF (macrophage colony-stimulating factor, R&D Systems), 50 ng / mL of RANKL (receptor activator of nuclear factor kappa B ligand, PeproTech Inc.) were added to all wells as Inducer of differentiation into osteoclasts. In addition, urolithin A (10 μM) was added simultaneously with this differentiation inducer. At 37°C, 5% CO 2 After standing still and culturing for 3 days in an incubator at a concentration of 100 mg / kg, the culture medium of each well was removed, and the cells were fixed in 10N Mildform for 10 minutes, and then washed 3 times with pure water. Then, 50 μL of TRAP staining solution (SIGMA) was added to each well, and incubated at 37° C. for 1 hour in a light-shielded state. The TRAP staining solution in each well was discarded, and after washing with pure water, 5...

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Abstract

The present invention addresses the problem of providing an effective and highly safe osteoclast differentiation inhibitor and a food or beverage, a medicine, a supplement, and a cosmetic exhibiting an osteoclast differentiation-inhibiting effect. The problem is solved by an osteoclast differentiation inhibitor containing an urolithin.

Description

technical field [0001] The present invention relates to a urolithin-containing osteoclast differentiation inhibitor, and specifically to a urolithin-containing differentiation inhibitor that inhibits the differentiation of hematopoietic stem cells into osteoclasts. Background technique [0002] In bone tissue, bone metabolism in which old bone is destroyed by osteoclasts (bone resorption) and new bone is formed by osteoblasts (bone formation) is continuously performed, and bone strength and blood calcium concentration are adjusted by this bone metabolism. If the balance between bone resorption and bone formation is disrupted, normal bone metabolism cannot be performed, resulting in bone diseases such as osteoporosis and bone metastasis. In bone remodeling (remodeling), bone metabolism is either increased, decreased, or normal, and in a pathological state in which bone resorption relatively exceeds bone formation, bone mass decreases. For example, osteoporosis includes hyper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/37A23L33/10A61P19/08A61P19/10A61P43/00
CPCA61P19/10A23L33/10A61K31/37A61P19/08A61P43/00A23L33/30A23L2/52A61K8/498A61K9/0095
Inventor 古旗贤二中谷祥惠工藤真丈
Owner DAICEL CHEM IND LTD
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