Preparation method of periplaneta americana polypeptide

A technology of Periplaneta americana and its extract, which is applied in the field of preparation of Periplaneta americana polypeptides, can solve the problems of low purity of Periplaneta americana polypeptides, and achieve the effects of process optimization, high purity and simple operation

Inactive Publication Date: 2020-10-09
石小和
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of this, the main purpose of the present invention is to provide a method for preparing a Periplaneta americana poly

Method used

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  • Preparation method of periplaneta americana polypeptide

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preparation example Construction

[0016] A method for preparing a Periplaneta americana polypeptide, comprising the steps of:

[0017] S01. Provide Periplaneta americana, degrease the Periplaneta americana, then use 40%-90% ethanol aqueous solution as the extract to extract the components, collect the extract, concentrate to a density of 1.10-1.15g / mL, and obtain the total Extract;

[0018] S02, provide water, mix the total extract with the water, carry out water precipitation, then filter, and collect the filtrate; perform gradient elution on the macroporous resin column on the filtrate, and the mobile phase used in the gradient elution Including: water, 70%-80% alcohol aqueous solution and at least one mass percentage concentration of alcohol aqueous solution lower than 70%, then collect the eluate of the 70%-80% alcohol aqueous solution, and concentrate to the volume of the eluent Below 0.1 times of that, the concentrated solution is obtained;

[0019] S03. Mix the concentrated solution with an organic so...

Embodiment 1

[0056] This embodiment provides a method for preparing a Periplaneta americana polypeptide, comprising the following steps:

[0057] 1. Take the adults of Periplaneta americana 15 days after stopping feeding, scald them with boiling water for 1 minute, dry them, spread them evenly on stainless steel trays, place them in an electric blast drying oven at 45°C and dry them for 48 hours, and then place them in a high-speed universal pulverizer Pulverize in, pass through 10 mesh sieves again, obtain Periplaneta americana pulverize.

[0058] 2. Soak the crushed Periplaneta americana (10kg) in petroleum ether (300L) at least 10 times the volume of the crushed Periplaneta americana, extract at 50°C for 2 hours, then repeat the extraction once in the same way, then filter, and then Remove sherwood oil, obtain the Periplaneta americana through degreasing treatment, stand-by.

[0059] 3. Using 8 times volume, 6 times volume and 8 times volume of 75% ethanol aqueous solution of Periplane...

Embodiment 2

[0063] This embodiment provides a method for preparing a Periplaneta americana polypeptide, comprising the following steps:

[0064] 1. Take the adults of Periplaneta americana 15 days after stopping feeding, scald them with boiling water for 1 minute, dry them in the air, spread them evenly on stainless steel trays, place them in an electric blast drying oven at 55°C and dry them for 48 hours, and then place them in a high-speed universal pulverizer Pulverize in, pass through 20 mesh sieves again, obtain Periplaneta americana pulverize.

[0065] 2. Soak the crushed Periplaneta americana (20kg) in petroleum ether (300L) at least 10 times the volume of the crushed Periplaneta americana, extract at 50°C for 2 hours, then repeat the extraction twice in the same way, then filter, Remove sherwood oil afterwards, obtain the Periplaneta americana through defatting process, stand-by.

[0066] 3. Using 8 times volume, 6 times volume and 8 times volume of 75% ethanol aqueous solution o...

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Abstract

The invention belongs to the technical field of polypeptide preparation, and particularly relates to a periplaneta americana polypeptide preparation method, which comprises: providing periplaneta americana, carrying out degreasing treatment on the periplaneta americana, carrying out component extraction by using a 65-85% ethanol aqueous solution as an extraction solution, collecting the extractionsolution, and concentrating to obtain a total extract; uniformly mixing the total extract with water, filtering, and collecting filtrate; putting the filtrate into a macroporous resin column, performing gradient elution by adopting a mobile phase comprising water, 70-80% alcohol-water solution and at least one alcohol-water solution of which the mass percentage concentration is lower than 70%, then collecting eluent of the 70-80% alcohol-water solution, and concentrating until the volume of the eluent is less than 0.1 time that of the eluent, so as to obtain a concentrated solution; mixing the concentrated solution with an organic solvent, extracting, collecting an organic solvent extracting solution, crystallizing the organic solvent extracting solution, collecting crystals, and drying;wherein the organic solvent is a mixed solvent of ethyl acetate and petroleum ether, and the mixing volume ratio of ethyl acetate to petroleum ether is (1-5): 1.

Description

technical field [0001] The invention belongs to the technical field of polypeptide preparation, and in particular relates to a preparation method of Periplaneta americana polypeptide. Background technique [0002] Periplaneta americana is an insect belonging to the genus Periplaneta of the family Blattidae of the order Blattata, commonly known as cockroach. It has been recorded in detail in the monographs of herbal medicines of all dynasties in my country, such as "Compendium of Materia Medica" and "Newly Revised Materia Medica". There are also studies and accounts. Periplaneta americana, dried and used as medicine, has high medicinal value. It has more than 200 important ingredients to match it. As a traditional Chinese medicine, it is cold in nature, salty in taste, and slightly poisonous. It has the effects of strengthening bones and tendons, reducing swelling and relieving pain, and is mainly used for lumbago and leg pain, bruises, arthritis and other diseases. Modern m...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/36C07K1/30C07K1/34
CPCC07K14/43563
Inventor 石小和
Owner 石小和
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