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A kind of high-temperature microbial composite bacterial agent for ramie biological degumming pretreatment and preparation method thereof

A compound bacterial agent and biological degumming technology, which is applied in the field of biological degumming, can solve the problems of polluted environment, waste of water resources, etc., and achieve the effect of solving polluted environment, consuming less energy and water, and good degumming effect

Active Publication Date: 2022-04-08
FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of this, the present invention provides a high-temperature microbial composite bacterial agent for ramie biological degumming pretreatment that can be produced in a large scale and has a good decomposition effect on colloidal components, so as to solve the waste of water resources in the traditional chemical degumming process of the textile industry And the difficult problem of polluting the environment; the present invention also provides the preparation method of this high-temperature microbial composite bacterial agent, and preparation method is simple, and cost is lower, can realize ramie biological degumming effectively

Method used

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  • A kind of high-temperature microbial composite bacterial agent for ramie biological degumming pretreatment and preparation method thereof
  • A kind of high-temperature microbial composite bacterial agent for ramie biological degumming pretreatment and preparation method thereof
  • A kind of high-temperature microbial composite bacterial agent for ramie biological degumming pretreatment and preparation method thereof

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Embodiment 1

[0029] Embodiment 1 of the present invention provides a preparation method of a high-temperature microbial composite inoculant for ramie biological degumming pretreatment, comprising the following steps:

[0030] (1), prepare the MRS medium with pH value of 7.0: weigh 90 g of peptone, 50 g of yeast extract, 85 g of beef extract, 200 g of glucose, 45 g of sodium acetate, 25 g of diammonium citrate, 10 g of Tween 80, 5 g of magnesium sulfate, Mix 0.5g manganese sulfate, 20g dipotassium hydrogen phosphate and 10L distilled water, sterilize at 121°C for 20min;

[0031] (2), prepare LB medium with pH value of 7.0: weigh 100 g of peptone, 80 g of yeast extract, 75 g of sodium chloride and 10 L of distilled water, mix, and sterilize at 121 °C for 20 min;

[0032] (3), prepare the MSM medium with pH value of 7.0: weigh 35g of sodium chloride, CaCO 3 15g, peptone 180g, yeast powder 10g, dipotassium hydrogen phosphate 10g, MgSO 4 ·7H 2 O 3g, FeSO 4 ·7H 2 O 0.04g, manganese sulfate...

Embodiment 2

[0052] Embodiment 2 of the present invention provides a kind of preparation method of the high-temperature microbial compound bacterial agent for ramie degumming, comprising the following steps:

[0053] (1), prepare the MRS medium with pH value of 7.2: weigh 100g of peptone, 45g of yeast extract, 95g of beef extract, 160g of glucose, 55g of sodium acetate, 28g of diammonium citrate, 12g of Tween 80, 6g of magnesium sulfate, Mix 0.4g of manganese sulfate, 18g of dipotassium hydrogen phosphate and 10L of distilled water, and sterilize at 121°C for 20min;

[0054] (2), prepare LB medium with pH value of 7.2: weigh 120g of peptone, 60g of yeast extract, 65g of sodium chloride and 10L of distilled water, mix, and sterilize at 121°C for 20min;

[0055] (3), prepare the MSM medium whose pH value is 7.2: weigh 45g of sodium chloride, CaCO 3 18g, peptone 160g, yeast powder 8g, dipotassium hydrogen phosphate 8g, MgSO 4 ·7H 2 O 2g, FeSO 4 ·7H 2 O 0.03g, manganese sulfate 0.01g, Zn...

Embodiment 3

[0065] Embodiment 3 of the present invention provides a kind of preparation method of the high temperature microbial compound bacterial agent for ramie degumming, comprises the following steps:

[0066] (1), prepare the MRS medium with pH value of 7.0: weigh 110g of peptone, 55g of yeast extract, 100g of beef extract, 220g of glucose, 55g of sodium acetate, 26g of diammonium citrate, 18g of Tween 80, 6g of magnesium sulfate, Mix 0.6g of manganese sulfate, 22g of dipotassium hydrogen phosphate and 10L of distilled water, and sterilize at 121°C for 20min;

[0067] (2), prepare LB medium with pH value of 7.0: weigh 135g of peptone, 90g of yeast extract, 95g of sodium chloride and 10L of distilled water, mix, and sterilize at 121°C for 20min;

[0068] (3), prepare the MSM medium whose pH value is 7.0: weigh 25g of sodium chloride, CaCO 3 20g, peptone 190g, yeast powder 6g, dipotassium hydrogen phosphate 11g, MgSO 4 ·7H 2 O 4g, FeSO 4 ·7H 2 O 0.05g, manganese sulfate 0.01g, Z...

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Abstract

The invention relates to the technical field of biological degumming, in particular to a preparation method of a high-temperature microbial composite bacterial agent used for pretreatment of ramie biological degumming. The preparation method comprises the following steps: respectively culturing Pediococcus pentosaceae, Bacillus pumilus, Geobacillus and Clostridium thermocellum in the culture medium to obtain the bacterial liquid of Pediococcus pentosaceae, the bacterial liquid of Bacillus pumilus and the bacterial liquid of Geobacillus pumilus Bacterial solution, Clostridium thermocellum bacterial solution, these four kinds of bacterial solutions are mixed to obtain a composite bacterial agent seed solution, and the composite bacterial agent seed solution is inserted into a composite bacterial agent medium for mixed fermentation to obtain a microbial composite bacterium agent. The preparation method provided by the invention is simple and low in cost, and can effectively realize biological degumming of ramie.

Description

technical field [0001] The invention relates to the technical field of biological degumming, in particular to a high-temperature microbial compound inoculant used for ramie biological degumming pretreatment and a preparation method thereof. Background technique [0002] Ramie (scientific name: Boehmeria nivea (L.) Gaudich.) is a subshrub or shrub of the genus Ramie of the Urticaceae family), and its fibers have the characteristics of high strength and high modulus, and are widely used in industrial composite materials. China is the country with the largest ramie production, accounting for about 90% of the world's total production. The raw hemp of ramie contains 20-30% of colloid. In a broad sense, colloid refers to the non-cellulose components in the raw hemp, including hemicellulose, pectin, lignin, water-soluble matter, fat wax, ash, etc. The degumming process in actual production is mainly the process of destroying the hemicellulose and pectin components to separate the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20D01C1/04C12R1/145C12R1/07C12R1/01
CPCC12N1/20D01C1/04
Inventor 王轶郭鹏
Owner FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI
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