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Method for extracting and separating baicalin from mixed micelles

A technology for mixing micelles and baicalin, which is applied in chemical instruments and methods, drug combinations, preparation of sugar derivatives, etc., to achieve the effects of changing ionic strength, promoting physical and chemical properties, and expanding extraction range

Active Publication Date: 2020-10-16
汉方萃取生物科技(海南)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no research on the extraction and separation of baicalin using a surfactant aqueous system

Method used

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  • Method for extracting and separating baicalin from mixed micelles
  • Method for extracting and separating baicalin from mixed micelles
  • Method for extracting and separating baicalin from mixed micelles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] (1) raw material pretreatment: use the traditional Chinese medicine pulverizer to pulverize the dried Scutellaria baicalensis root, and pass through a 60-mesh sieve to obtain the dried Scutellaria baicalensis root powder;

[0062] (2) Preparation of extractant: Add 10g of nonionic surfactant Genapol X-080 into 100mL of deionized water, add 0.01g of Gemini surfactant 16-5-16 into 100mL of deionized water, stir evenly at room temperature, get the extractant;

[0063] (3) Scutellaria baicalensis root powder is mixed with extractant: Weigh 1g of dried scutellaria baicalensis root powder in step (1), 10ml of extractant in step (2), mix, stir at room temperature for 30min, and use a centrifuge at 8000r / min Centrifuge at a speed of 10 min to obtain the supernatant A 1 ;

[0064] (4) Pre-concentration of baicalin: get 5% (w / v) sodium chloride and add supernatant A 1 Medium (5g sodium chloride / 100mL supernatant A 1 ), extracted in a constant temperature water bath at 45°C fo...

Embodiment 2

[0068] (1) raw material pretreatment: same as step (1) in embodiment 1;

[0069] (2) Preparation of extractant: Add 12g of nonionic surfactant Genapol X-080 into 100mL of deionized water, add 0.01g of Gemini surfactant 16-5-16 into 100mL of deionized water, stir evenly at room temperature, get the extractant;

[0070] (3)-(5) are the same as (3)-(5) in Example 1 to obtain solid D 2 .

[0071] take solid D 2 Add methanol solution to configure a solution with a concentration of 10 μg / ml, and use high-performance liquid chromatography to detect, Figure 4 For the baicalin sample D isolated in this embodiment 2 Analytical HPLC chromatogram. Calculate D 2 The purity is 86.4%.

Embodiment 3

[0073] (1) raw material pretreatment: same as step (1) in embodiment 2;

[0074] (2) Preparation of extractant: Add 12g of nonionic surfactant Genapol X-080 into 100mL of deionized water, add 0.03g of Gemini surfactant 16-5-16 into 100mL of deionized water, stir evenly at room temperature, get the extractant;

[0075] (3)~(5) are the same as (3)~(5) in Example 2, obtain solid D 3 .

[0076] take solid D 3 Add methanol solution to configure a solution with a concentration of 10 μg / ml, and use high-performance liquid chromatography to detect, Figure 5 For the baicalin sample D isolated in this embodiment 3 Analytical HPLC chromatogram. Calculate D 3 The purity is 92.1%.

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Abstract

The invention discloses a method for extracting and separating baicalin from mixed micelles, and belongs to the technical field of extraction and separation of natural products. The method mainly comprises two steps of I, effectively extracting baicalin with the mixed micelles formed from a nonionic surface active agent Genapol X-080 and a Gemini surface active agent 16-5-16; and II, performing purification and reverse extraction on the baicalin in the micelles by a "like dissolving like" principle, and lowering the temperature for crystallizing to obtain baicalin solids. The mixed micelles have good transmission capacity for polyhydroxy containing baicalin. Compared with a conventional solvent extraction method, the organic solvent used by the method and the acid water content are lower,and a green chemistry purpose is met. The purity of the baicalin obtained by separation by the method is as high as 92.4%, and the baicalin has high practical application value.

Description

technical field [0001] The invention relates to a method for extracting and separating baicalin by using mixed micelles, and belongs to the technical field of extraction and separation of natural products. Background technique [0002] Baicalin (BC) is a flavonoid compound, the main active ingredient of Scutellaria baicalensis, which has a wide range of biological and pharmacological activities, such as antibacterial, anti-inflammatory, antioxidant, diuretic, sedative, antihypertensive, immunosuppressive, etc. In recent years, research on the anti-tumor effect of baicalin has been increasing. Studies have shown that baicalin can inhibit the proliferation of cells such as Huh-7, BEL-7402, and HLF, and induce the apoptosis of BEL-7402, SMMC-7721, and HepG-2 cells. At the same time, it can also assist other drugs to improve or even cure by affecting the tumor cell cycle, up-regulating pro-apoptotic genes, changing the permeability of mitochondrial membranes, organizing DNA synt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H17/07A61P7/10A61P9/12A61P11/00A61P25/20A61P31/04A61P35/00A61P37/06B01F17/00C09K23/00
CPCC07H1/08C07H17/07A61P35/00A61P11/00A61P31/04A61P7/10A61P25/20A61P9/12A61P37/06C09K23/018
Inventor 王海军李叶惠李晓宁王祥
Owner 汉方萃取生物科技(海南)股份有限公司
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