Method for culturing carbapenem-resistant escherichia coli

Abstract

The invention relates to the field of escherichia coli culture, in particular to a method for culturing carbapenem-resistant escherichia coli. The method comprises a culture bottle body, a constant-temperature bottle seat, a shading assembly, a filtering assembly, a locking assembly and scale marks, the bottom of the culture bottle body is mounted on the constant-temperature bottle seat, the upperend of the constant-temperature bottle seat and the culture bottle body are fixedly mounted through the locking assembly, the shading assembly is mounted at one end, far from the constant-temperaturebottle seat, of the culture bottle body, and the filtering assembly is detachably mounted in the culture bottle body. The culture bottle body consists of a lower bottle body and an upper bottle body,solves the problems of adding a culture medium from a bottleneck and causing pollution to the bottleneck, and omits operation on the bottleneck during culture operation. The operation is convenient,the culture medium is prevented from making contact with the side wall of the lower bottle body, filtering treatment on the culture medium is achieved, constant-temperature control over the culture medium in the lower bottle body is achieved through the constant-temperature bottle seat, shading cloth is installed on the culture bottle to conduct shading treatment on the culture medium, and use ismore flexible.

Description

technical field [0001] The invention relates to the field of Escherichia coli cultivation, in particular to a method for cultivating carbapenem-resistant Escherichia coli. Background technique [0002] Carbapenem-resistant E. coli, a superbug that kills half of those it infects, is rampant in U.S. healthcare settings, and carbapenem-resistant Enterobacteriaceae are actually a group of enterobacteria that include There are more than 70 kinds of bacteria. This kind of bacteria is called "super bacteria" because it is resistant to many new antibiotics, and the mortality rate of infected people is very high. The in vitro culture of cells and tissues has become a life research and An essential part of practice, a wide variety of cell types are cultivated, from viruses to bacteria and fungi, from human cells to animal cells and plant cells, and culture flasks are used for the cultivation of carbapenem-resistant E. coli. [0003] The culture bottle for carbapenem-resistant Escheri...

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Problems solved by technology

[0003] The culture bottle for carbapenem-resistant Escherichia coli is set up as a whole. The medium is added from the mouth of the bottle to the inside of the bottle. During the addition process, the inner wall of the bottle mouth will be polluted, so it is necessary to close the bottle mouth during operation. To clean it, at the same time, it is necessary to install tampons on the mouth of the bottle. The operation is complicated, and the cultivation temperature is lower than 37°C, which affects the efficiency of cultivation. When observing the culture status, the shading of the culture bottle was destroyed, and the shading effect was not ideal

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