Method for simultaneously determining various arsenic forms in food

Abstract

The invention discloses a method for simultaneously determining multiple arsenic-form compounds. Arsenic in food is oscillated, extracted and purified by a shaking table, then enters a high performance liquid chromatograph, and is subjected to gradient elution separation by mobile phases 0.5 mmol / L (NH4) 2CO3 (pH 8.5) and 50mmol / L (NH4) 2CO3 (pH 8.5), an inductively coupled plasma mass spectrometer is used to detect arsenic forms separated at different times, the retention time is used for qualitative determination, and a peak area external standard method is used for quantification. The method can be used for simultaneously determining nine arsenic forms in food, comprising arsenious acid [As (III)], arsenic acid [As (V)], monomethyl arsenic (MMA), dimethyl arsenic (DMA), arsenic betaine(AsB), arsenic choline (AsC), 4-aminobenzene arsenic acid (p-ASA), roxarsone (ROX) and 2-aminobenzene arsenic acid (o-ASA). The method is rapid, simple, accurate, sensitive and good in reproducibility, makes up the defect that standards and literatures can only measure 4-6 arsenic forms at the same time generally, has important practical significance in reasonably evaluating food quality and safety, and is suitable for popularization and application in detection institutions or laboratories.

Description

technical field [0001] The invention relates to a method for determining morphological compounds, in particular to a method for analyzing and determining various forms of arsenic in food, and belongs to the technical field of chemical detection and analysis. Background technique [0002] Arsenic is an element widely distributed in nature. Common arsenic compounds include: arsenous acid [As(III)], arsenic acid [As(V)], monomethylarsenic acid (MMA), dimethylarsenic acid (DMA ), arsenobetaine (AsB), arsenocholine (AsC), 4-aminophenylarsenic acid (p-ASA), roxarsarsenic acid (ROX), 2-aminophenylarsenic acid (o-ASA). The biological toxicity of arsenic is not only related to its content, but also determined by its existing form to a greater extent. The toxicity of different arsenic forms varies greatly. Inorganic arsenic is the most toxic, and organic arsenic is less toxic. The more arsenic is combined with organic groups, the less toxic it is. Usually, arsenobetaine and arsenocho...

AI Technical Summary

Problems solved by technology

In the national standard, liquid chromatography (HPLC)-atomic fluorescence (AFS) combined system is used to detect food. This method can only detect arsenous acid [As(III)], arsenic acid [As(V)], There are four forms of arsenic, methyl arsenic acid (MMA) and dimethyl arsenic acid (DMA), which cannot be detected for arsenobetaine (AsB), which is often the dominant component in food: In addition, the second method of the national standard uses liquid chromatography (HPLC) -Inductively coupled plasma mass spectrometry (ICP-MS) method can only detect arsenous acid [As(III)], arsenic acid [As(V)], monomethyl arsenic acid (MMA) and dimethyl arsenic acid arsenic acid (DMA) and arsenobetaine (AsB), and it cannot detect arsenic choline (AsC), which is a common arsenic compound in seafood, especially in seafood.

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