Culture medium and method for propagating Alsophila costularis Baker by using same

A culture substrate, Cyphila sinensis technology, applied in the field of plant propagation, can solve the problems of difficult operation, low survival rate, high cost, etc., and achieve the effect of easy operation, high survival rate and more operation

Pending Publication Date: 2020-10-30
HARBIN NORMAL UNIVERSITY
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of difficult operation, high cost, low survival rate and difficulty in training seedlings in the artificial pro

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culture medium and method for propagating Alsophila costularis Baker by using same
  • Culture medium and method for propagating Alsophila costularis Baker by using same
  • Culture medium and method for propagating Alsophila costularis Baker by using same

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0027] Example 1. Propagation of Alsophila sinensis using 1 / 2MS culture medium

[0028] 1. Configuration of 1 / 2MS culture medium

[0029] 1 / 2MS culture medium consists of soot and humus soil moistened by 1 / 2MS culture solution. The composition of 1 / 2MS culture solution is the same as that of MS culture solution. Among them, the content of macro elements and trace elements are both MS culture solution The content of iron salt and organic components is the same as that of MS culture solution, the pH is 5.5-6, and the mass of soot is 3% of the mass of humus soil wetted by 1 / 2 MS culture solution.

[0030] 2. Production of 1 / 2MS culture medium

[0031] Sterilize a culture plate with a length of 35 cm, a width of 20 cm, and a height of 3 cm at 125°C for 20 minutes by autoclaving. Spread a sponge with a thickness of 5 mm on the culture plate, and then spread humus soil with a thickness of about 2 cm on the sponge and flatten it. 、Compact, wet with 1 / 2MS culture solution, the mass ratio of ...

Example Embodiment

[0038] Example 2: Propagation of Alsophila sinensis using 1 / 8MS culture medium

[0039] 1. Configuration of 1 / 8MS culture medium

[0040] The 1 / 8MS culture medium is composed of soot and the humic map wetted by 1 / 8MS culture solution. The composition of 1 / 8MS culture solution is the same as that of MS culture solution. Among them, the contents of macroelements and trace elements are all MS culture solution. The content of iron salt and organic components is the same as that of MS culture solution, the pH is 5.5-6, and the mass of soot is 3% of the mass of humus soil wetted by 1 / 8 MS culture solution.

[0041] 2. Production of 1 / 8MS culture medium

[0042] Sterilize a culture dish with a length of 35 cm, a width of 20 cm, and a height of 3 cm at 125°C for 20 min by autoclaving. Spread a sponge with a thickness of 5 mm on the culture dish, and then spread humus soil with a thickness of about 2 cm on the sponge and flatten it. , Compaction, wetting with 1 / 8MS culture solution, the mass ...

Example Embodiment

[0049] Example 3. Propagation of Alsophila sinensis using the full amount of MS culture medium

[0050] 1. The configuration of full MS culture medium

[0051] The MS culture medium is composed of soot and humus soil moistened by the MS culture solution. The composition of the MS culture solution is the same as that of the MS culture solution. The contents of macro and trace elements are the same as that of the MS culture solution. The content of iron salt and organic components is the same as the MS culture solution, the pH is 5.5-6, and the mass of soot is 3% of the mass of the humus soil wetted by the entire MS culture solution.

[0052] 2. Production of full MS culture medium

[0053] Sterilize a culture plate with a length of 35 cm, a width of 20 cm, and a height of 3 cm at 125°C for 20 minutes by autoclaving. Spread a sponge with a thickness of 5 mm on the culture plate, and then spread humus soil with a thickness of about 2 cm on the sponge and flatten it. , Compact, wet with ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Login to view more

Abstract

The invention discloses a culture medium and a method for propagating Alsophila costularis Baker by using the same, belonging to the technical field of plant propagation methods. The invention aims tosolve the problems of difficult operation, high cost, difficult seedling exercising, low survival rate and the like in the existing artificial propagation process of Alsophila costularis Baker. The culture medium is composed of coal ash and humus wetted by a culture solution; the culture medium is inoculated with disinfected spores of Alsophila costularis Baker and the spores of Alsophila costularis Baker are cultured, and finally mature sporophyte seedlings are obtained. By means of the method, the propagation process of the Alsophila costularis Baker is easier to operate, and the survival rate is higher. When the method is used for breeding the Alsophila costularis Baker, spores can germinate after 6-9 days averagely, gametophytes are formed after 28-37 days, juvenile sporophyte seedlings can be formed after 62-71 days, and the transplanting survival rate of the juvenile sporophyte seedlings can reach 98%.

Description

technical field [0001] The invention relates to a culture substrate and a method for propagating Cymphila sinensis by using it, and belongs to the technical field of plant propagation methods. Background technique [0002] Alsophila costularis Baker (Alsophila costularis Baker) is a relict plant with an ancient origin and has the title of "living fossil". It is of great value to the study of ancient ecological changes and plant system evolution, and at the same time predicts the trend of ecosystem changes. , Assessing the ecological pressure caused by economic activities and monitoring environmental pollution has an important indicative role. Alsophilaceae plants have a beautiful tree shape, and the crown of the tree is like a giant umbrella, which is of high horticultural ornamental value. Alsophilaceae plants can also be used medicinally, and their rhizomes have the functions of clearing heat, detoxifying, and dispelling rheumatism. Orchid cultivators in my country often...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01G17/00A01G24/10A01G24/28A01G24/30
CPCA01G17/005A01G24/10A01G24/28A01G24/30
Inventor 刘保东邓里涛肖湘王梓承
Owner HARBIN NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap