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Culture medium and method for propagating Alsophila costularis Baker by using same

A culture substrate, Cyphila sinensis technology, applied in the field of plant propagation, can solve the problems of difficult operation, low survival rate, high cost, etc., and achieve the effect of easy operation, high survival rate and more operation

Pending Publication Date: 2020-10-30
HARBIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of difficult operation, high cost, low survival rate and difficulty in training seedlings in the artificial propagation process of Cyasophila chinensis, the present invention provides a culture substrate and a method for propagating Cyassina chinensis using it

Method used

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  • Culture medium and method for propagating Alsophila costularis Baker by using same
  • Culture medium and method for propagating Alsophila costularis Baker by using same
  • Culture medium and method for propagating Alsophila costularis Baker by using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, using 1 / 2 MS culture substrate to propagate Cyphila sinensis

[0028] 1. Configuration of 1 / 2MS culture substrate

[0029] The 1 / 2MS culture medium is composed of coal soot and 1 / 2MS culture medium moistened humus, and the composition of 1 / 2MS culture medium is the same as that of MS culture medium. The content of iron salt and organic components is the same as that of MS culture solution, the pH is 5.5-6, and the quality of coal soot is 3% of the mass of humus soil wetted by 1 / 2 MS culture solution.

[0030] 2. Production of 1 / 2MS culture substrate

[0031] Sterilize a 35 cm long, 20 cm wide, and 3 cm high culture tray at 125°C for 20 minutes, spread a sponge with a thickness of 5 mm on the culture tray, and spread humus soil about 2 cm thick on the sponge, and press it flat , compacted, moistened with 1 / 2MS culture solution, the mass ratio of culture solution to humus soil is 1:10, then mixed with coal soot and mixed evenly, and finally put the culture...

Embodiment 2

[0038] Embodiment 2, using 1 / 8MS culture medium to propagate Cyphila sinensis

[0039] 1. Configuration of 1 / 8MS culture substrate

[0040] The 1 / 8MS culture substrate is composed of soot and 1 / 8MS culture fluid wetted humic pattern, the composition of 1 / 8MS culture fluid is the same as MS culture fluid, and the content of macroelements and trace elements are all in MS culture fluid The content of iron salt and organic components is the same as that of MS culture solution, the pH is 5.5-6, and the quality of coal soot is 3% of the mass of humus wetted by 1 / 8 MS culture solution.

[0041] 2. Production of 1 / 8MS culture substrate

[0042] Sterilize a 35 cm long, 20 cm wide, and 3 cm high culture tray at 125°C for 20 minutes, spread a sponge with a thickness of 5 mm on the culture tray, and spread humus soil about 2 cm thick on the sponge, and press it flat , compacted, moistened with 1 / 8MS culture solution, the mass ratio of culture solution to humus soil is 1:10, then mixed w...

Embodiment 3

[0049] Example 3, using the full amount of MS culture substrate to propagate Cyphila sinensis

[0050] 1. Configuration of full MS culture substrate

[0051] The full amount of MS culture medium is composed of soot and humus moistened by the full amount of MS culture solution. The composition of the full amount of MS culture solution is the same as that of MS culture solution, and the content of macroelements and trace elements is the same as that of MS culture solution. The content of iron salt and organic components is the same as that of MS culture solution, the pH is 5.5-6, and the quality of coal soot is 3% of the mass of humus soil wetted by the full amount of MS culture solution.

[0052] 2. Production of full MS culture substrate

[0053] Sterilize a 35 cm long, 20 cm wide, and 3 cm high culture tray at 125°C for 20 minutes, spread a sponge with a thickness of 5 mm on the culture tray, and spread humus soil about 2 cm thick on the sponge, and press it flat , compacte...

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Abstract

The invention discloses a culture medium and a method for propagating Alsophila costularis Baker by using the same, belonging to the technical field of plant propagation methods. The invention aims tosolve the problems of difficult operation, high cost, difficult seedling exercising, low survival rate and the like in the existing artificial propagation process of Alsophila costularis Baker. The culture medium is composed of coal ash and humus wetted by a culture solution; the culture medium is inoculated with disinfected spores of Alsophila costularis Baker and the spores of Alsophila costularis Baker are cultured, and finally mature sporophyte seedlings are obtained. By means of the method, the propagation process of the Alsophila costularis Baker is easier to operate, and the survival rate is higher. When the method is used for breeding the Alsophila costularis Baker, spores can germinate after 6-9 days averagely, gametophytes are formed after 28-37 days, juvenile sporophyte seedlings can be formed after 62-71 days, and the transplanting survival rate of the juvenile sporophyte seedlings can reach 98%.

Description

technical field [0001] The invention relates to a culture substrate and a method for propagating Cymphila sinensis by using it, and belongs to the technical field of plant propagation methods. Background technique [0002] Alsophila costularis Baker (Alsophila costularis Baker) is a relict plant with an ancient origin and has the title of "living fossil". It is of great value to the study of ancient ecological changes and plant system evolution, and at the same time predicts the trend of ecosystem changes. , Assessing the ecological pressure caused by economic activities and monitoring environmental pollution has an important indicative role. Alsophilaceae plants have a beautiful tree shape, and the crown of the tree is like a giant umbrella, which is of high horticultural ornamental value. Alsophilaceae plants can also be used medicinally, and their rhizomes have the functions of clearing heat, detoxifying, and dispelling rheumatism. Orchid cultivators in my country often...

Claims

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Application Information

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IPC IPC(8): A01G17/00A01G24/10A01G24/28A01G24/30
CPCA01G17/005A01G24/10A01G24/28A01G24/30
Inventor 刘保东邓里涛肖湘王梓承
Owner HARBIN NORMAL UNIVERSITY
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